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Microbial oil-degradation under mild hydrostatic pressure (10 MPa) : which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?

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  • 41MO8912U
Abstract
Oil spills represent an overwhelming carbon input to the marine environment that immediately impacts the sea surface ecosystem. Microbial communities degrading the oil fraction that eventually sinks to the seafloor must also deal with hydrostatic pressure, which linearly increases with depth. Piezosensitive hydrocarbonoclastic bacteria are ideal candidates to elucidate impaired pathways following oil spills at low depth. In the present paper, we tested two strains of the ubiquitous Alcanivorax genus, namely A. jadensis KS_339 and A. dieselolei KS_293, which is known to rapidly grow after oil spills. Strains were subjected to atmospheric and mild pressure (0.1, 5 and 10 MPa, corresponding to a depth of 0, 500 and 1000 m, respectively) providing n-dodecane as sole carbon source. Pressures equal to 5 and 10 MPa significantly lowered growth yields of both strains. However, in strain KS_293 grown at 10 MPa CO2 production per cell was not affected, cell integrity was preserved and PO43- uptake increased. Analysis of its transcriptome revealed that 95% of its genes were downregulated. Increased transcription involved protein synthesis, energy generation and respiration pathways. Interplay between these factors may play a key role in shaping the structure of microbial communities developed after oil spills at low depth and limit their bioremediation potential.
Keywords
DEEP-WATER-HORIZON, DEGRADING BACTERIA, PROTEIN-SYNTHESIS, ESCHERICHIA-COLI, FLOW-CYTOMETRY, SEA BACTERIA, SP. NOV., IN-VIVO, GROWTH, INHIBITION

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MLA
Scoma, Alberto et al. “Microbial Oil-degradation Under Mild Hydrostatic Pressure (10 MPa) : Which Pathways Are Impacted in Piezosensitive Hydrocarbonoclastic Bacteria?” SCIENTIFIC REPORTS 6 (2016): n. pag. Print.
APA
Scoma, A., Barbato, M., Hernandez Sanabria, E., Mapelli, F., Daffonchio, D., Borin, S., & Boon, N. (2016). Microbial oil-degradation under mild hydrostatic pressure (10 MPa) : which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria? SCIENTIFIC REPORTS, 6.
Chicago author-date
Scoma, Alberto, Marta Barbato, Emma Hernandez Sanabria, Francesca Mapelli, Daniele Daffonchio, Sara Borin, and Nico Boon. 2016. “Microbial Oil-degradation Under Mild Hydrostatic Pressure (10 MPa) : Which Pathways Are Impacted in Piezosensitive Hydrocarbonoclastic Bacteria?” Scientific Reports 6.
Chicago author-date (all authors)
Scoma, Alberto, Marta Barbato, Emma Hernandez Sanabria, Francesca Mapelli, Daniele Daffonchio, Sara Borin, and Nico Boon. 2016. “Microbial Oil-degradation Under Mild Hydrostatic Pressure (10 MPa) : Which Pathways Are Impacted in Piezosensitive Hydrocarbonoclastic Bacteria?” Scientific Reports 6.
Vancouver
1.
Scoma A, Barbato M, Hernandez Sanabria E, Mapelli F, Daffonchio D, Borin S, et al. Microbial oil-degradation under mild hydrostatic pressure (10 MPa) : which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria? SCIENTIFIC REPORTS. 2016;6.
IEEE
[1]
A. Scoma et al., “Microbial oil-degradation under mild hydrostatic pressure (10 MPa) : which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{7198985,
  abstract     = {Oil spills represent an overwhelming carbon input to the marine environment that immediately impacts the sea surface ecosystem. Microbial communities degrading the oil fraction that eventually sinks to the seafloor must also deal with hydrostatic pressure, which linearly increases with depth. Piezosensitive hydrocarbonoclastic bacteria are ideal candidates to elucidate impaired pathways following oil spills at low depth. In the present paper, we tested two strains of the ubiquitous Alcanivorax genus, namely A. jadensis KS_339 and A. dieselolei KS_293, which is known to rapidly grow after oil spills. Strains were subjected to atmospheric and mild pressure (0.1, 5 and 10 MPa, corresponding to a depth of 0, 500 and 1000 m, respectively) providing n-dodecane as sole carbon source. Pressures equal to 5 and 10 MPa significantly lowered growth yields of both strains. However, in strain KS_293 grown at 10 MPa CO2 production per cell was not affected, cell integrity was preserved and PO43- uptake increased. Analysis of its transcriptome revealed that 95% of its genes were downregulated. Increased transcription involved protein synthesis, energy generation and respiration pathways. Interplay between these factors may play a key role in shaping the structure of microbial communities developed after oil spills at low depth and limit their bioremediation potential.},
  articleno    = {23526},
  author       = {Scoma, Alberto and Barbato, Marta and Hernandez Sanabria, Emma and Mapelli, Francesca and Daffonchio, Daniele and Borin, Sara and Boon, Nico},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {DEEP-WATER-HORIZON,DEGRADING BACTERIA,PROTEIN-SYNTHESIS,ESCHERICHIA-COLI,FLOW-CYTOMETRY,SEA BACTERIA,SP. NOV.,IN-VIVO,GROWTH,INHIBITION},
  language     = {eng},
  pages        = {14},
  title        = {Microbial oil-degradation under mild hydrostatic pressure (10 MPa) : which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?},
  url          = {http://dx.doi.org/10.1038/srep23526},
  volume       = {6},
  year         = {2016},
}

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