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A combined binary interaction and phenotypic map of C. elegans cell polarity proteins

(2016) NATURE CELL BIOLOGY. 18(3). p.337-346
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Abstract
The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode Caenorhabditis elegans. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the C. elegans embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.
Keywords
NETWORK, EARLY EMBRYOGENESIS, YEAST, CAENORHABDITIS-ELEGANS, DROSOPHILA, 2-HYBRID, GENE, HOMOLOG, REQUIRES, EMBRYOS

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Chicago
Koorman, Thijs, Diana Klompstra, Monique van der Voet, Irma Lemmens, João J Ramalho, Susan Nieuwenhuize, Sander van den Heuvel, Jan Tavernier, Jeremy Nance, and Mike Boxem. 2016. “A Combined Binary Interaction and Phenotypic Map of C. Elegans Cell Polarity Proteins.” Nature Cell Biology 18 (3): 337–346.
APA
Koorman, T., Klompstra, D., van der Voet, M., Lemmens, I., Ramalho, J. J., Nieuwenhuize, S., van den Heuvel, S., et al. (2016). A combined binary interaction and phenotypic map of C. elegans cell polarity proteins. NATURE CELL BIOLOGY, 18(3), 337–346.
Vancouver
1.
Koorman T, Klompstra D, van der Voet M, Lemmens I, Ramalho JJ, Nieuwenhuize S, et al. A combined binary interaction and phenotypic map of C. elegans cell polarity proteins. NATURE CELL BIOLOGY. 2016;18(3):337–46.
MLA
Koorman, Thijs et al. “A Combined Binary Interaction and Phenotypic Map of C. Elegans Cell Polarity Proteins.” NATURE CELL BIOLOGY 18.3 (2016): 337–346. Print.
@article{7195271,
  abstract     = {The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode Caenorhabditis elegans. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the C. elegans embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.},
  author       = {Koorman, Thijs and Klompstra, Diana and van der Voet, Monique and Lemmens, Irma and Ramalho, João J and Nieuwenhuize, Susan and van den Heuvel, Sander and Tavernier, Jan and Nance, Jeremy and Boxem, Mike},
  issn         = {1465-7392},
  journal      = {NATURE CELL BIOLOGY},
  keywords     = {NETWORK,EARLY EMBRYOGENESIS,YEAST,CAENORHABDITIS-ELEGANS,DROSOPHILA,2-HYBRID,GENE,HOMOLOG,REQUIRES,EMBRYOS},
  language     = {eng},
  number       = {3},
  pages        = {337--346},
  title        = {A combined binary interaction and phenotypic map of C. elegans cell polarity proteins},
  url          = {http://dx.doi.org/10.1038/ncb3300},
  volume       = {18},
  year         = {2016},
}

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