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Analytical validation of a high-sensitivity cardiac troponin T assay in horses

Nicky Van Der Vekens, Marie-Astrid van Dievoet, Hendrik De Puydt, Annelies Decloedt UGent, Sofie Ven UGent, Dominique De Clercq UGent, Piet Deprez UGent and Gunther van Loon UGent (2015) JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 27(4). p.504-509
abstract
Although cardiac troponin T (cTnT) assays have been used to detect myocardial damage in horses, a cTnT assay has not been analytically validated, to our knowledge. The aims of this study were to estimate the precision of a high-sensitivity cTnT assay in horses and determine the effect of hemolysis on the measured cTnT concentration. Serum samples from horses were mixed in 3 different pools. Pool 1 consisted of samples from 3 healthy horses, pool 2 from 6 horses with heart failure or atypical myopathy, and pool 3 from 10 horses with atypical myopathy. The within- and between-run coefficients of variation were determined for each pool. Pools 2 and 3 were diluted to estimate linearity. To study the influence of sample hemolysis, serum was collected from 4 horses with a high cTnT concentration, in which hemolysis was mechanically induced. In addition, ethylenediamine tetra-acetic acid blood tubes were collected from 3 other horses, from which hemolysate was prepared and added to plasma at different concentrations. The within- and between-run coefficients of variation of all pools were <10%, and a good linearity was found. Three out of 4 hemolyzed serum samples had a decreased serum cTnT concentration. Plasma samples with a high hemolysis index showed a negative interference, resulting in a lower cTnT concentration. Results of the high-sensitivity cTnT assay were highly reproducible. Because samples from horses with musculoskeletal damage were included, further studies should test the possible cross-reactivity between troponin T of musculoskeletal and cardiac origin before the assay can be used in equine clinical practice.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SKELETAL-MUSCLE, I CONCENTRATIONS, HEMOLYSIS, DISEASE, SPECIMENS, INJURY, SERUM, MYOCARDIAL DAMAGE, ATYPICAL MYOPATHY, equine, cardiomyopathy, Cardiac biomarkers, cardiac troponin I
journal title
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
J. Vet. Diagn. Invest.
volume
27
issue
4
pages
504 - 509
Web of Science type
Article
Web of Science id
000358890900012
JCR category
VETERINARY SCIENCES
JCR impact factor
1.196 (2015)
JCR rank
45/138 (2015)
JCR quartile
2 (2015)
ISSN
1040-6387
DOI
10.1177/1040638715593601
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7193206
handle
http://hdl.handle.net/1854/LU-7193206
date created
2016-04-25 12:23:42
date last changed
2016-12-19 15:47:30
@article{7193206,
  abstract     = {Although cardiac troponin T (cTnT) assays have been used to detect myocardial damage in horses, a cTnT assay has not been analytically validated, to our knowledge. The aims of this study were to estimate the precision of a high-sensitivity cTnT assay in horses and determine the effect of hemolysis on the measured cTnT concentration. Serum samples from horses were mixed in 3 different pools. Pool 1 consisted of samples from 3 healthy horses, pool 2 from 6 horses with heart failure or atypical myopathy, and pool 3 from 10 horses with atypical myopathy. The within- and between-run coefficients of variation were determined for each pool. Pools 2 and 3 were diluted to estimate linearity. To study the influence of sample hemolysis, serum was collected from 4 horses with a high cTnT concentration, in which hemolysis was mechanically induced. In addition, ethylenediamine tetra-acetic acid blood tubes were collected from 3 other horses, from which hemolysate was prepared and added to plasma at different concentrations. The within- and between-run coefficients of variation of all pools were {\textlangle}10\%, and a good linearity was found. Three out of 4 hemolyzed serum samples had a decreased serum cTnT concentration. Plasma samples with a high hemolysis index showed a negative interference, resulting in a lower cTnT concentration. Results of the high-sensitivity cTnT assay were highly reproducible. Because samples from horses with musculoskeletal damage were included, further studies should test the possible cross-reactivity between troponin T of musculoskeletal and cardiac origin before the assay can be used in equine clinical practice.},
  author       = {Van Der Vekens, Nicky and van Dievoet, Marie-Astrid and De Puydt, Hendrik and Decloedt, Annelies and Ven, Sofie and De Clercq, Dominique and Deprez, Piet and van Loon, Gunther},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {SKELETAL-MUSCLE,I CONCENTRATIONS,HEMOLYSIS,DISEASE,SPECIMENS,INJURY,SERUM,MYOCARDIAL DAMAGE,ATYPICAL MYOPATHY,equine,cardiomyopathy,Cardiac biomarkers,cardiac troponin I},
  language     = {eng},
  number       = {4},
  pages        = {504--509},
  title        = {Analytical validation of a high-sensitivity cardiac troponin T assay in horses},
  url          = {http://dx.doi.org/10.1177/1040638715593601},
  volume       = {27},
  year         = {2015},
}

Chicago
Van Der Vekens, Nicky, Marie-Astrid van Dievoet, Hendrik De Puydt, Annelies Decloedt, Sofie Ven, Dominique De Clercq, Piet Deprez, and Gunther van Loon. 2015. “Analytical Validation of a High-sensitivity Cardiac Troponin T Assay in Horses.” Journal of Veterinary Diagnostic Investigation 27 (4): 504–509.
APA
Van Der Vekens, N., van Dievoet, M.-A., De Puydt, H., Decloedt, A., Ven, S., De Clercq, D., Deprez, P., et al. (2015). Analytical validation of a high-sensitivity cardiac troponin T assay in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 27(4), 504–509.
Vancouver
1.
Van Der Vekens N, van Dievoet M-A, De Puydt H, Decloedt A, Ven S, De Clercq D, et al. Analytical validation of a high-sensitivity cardiac troponin T assay in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2015;27(4):504–9.
MLA
Van Der Vekens, Nicky, Marie-Astrid van Dievoet, Hendrik De Puydt, et al. “Analytical Validation of a High-sensitivity Cardiac Troponin T Assay in Horses.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 27.4 (2015): 504–509. Print.