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Analytical validation of a high-sensitivity cardiac troponin T assay in horses

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Abstract
Although cardiac troponin T (cTnT) assays have been used to detect myocardial damage in horses, a cTnT assay has not been analytically validated, to our knowledge. The aims of this study were to estimate the precision of a high-sensitivity cTnT assay in horses and determine the effect of hemolysis on the measured cTnT concentration. Serum samples from horses were mixed in 3 different pools. Pool 1 consisted of samples from 3 healthy horses, pool 2 from 6 horses with heart failure or atypical myopathy, and pool 3 from 10 horses with atypical myopathy. The within- and between-run coefficients of variation were determined for each pool. Pools 2 and 3 were diluted to estimate linearity. To study the influence of sample hemolysis, serum was collected from 4 horses with a high cTnT concentration, in which hemolysis was mechanically induced. In addition, ethylenediamine tetra-acetic acid blood tubes were collected from 3 other horses, from which hemolysate was prepared and added to plasma at different concentrations. The within- and between-run coefficients of variation of all pools were <10%, and a good linearity was found. Three out of 4 hemolyzed serum samples had a decreased serum cTnT concentration. Plasma samples with a high hemolysis index showed a negative interference, resulting in a lower cTnT concentration. Results of the high-sensitivity cTnT assay were highly reproducible. Because samples from horses with musculoskeletal damage were included, further studies should test the possible cross-reactivity between troponin T of musculoskeletal and cardiac origin before the assay can be used in equine clinical practice.
Keywords
SKELETAL-MUSCLE, I CONCENTRATIONS, HEMOLYSIS, DISEASE, SPECIMENS, INJURY, SERUM, MYOCARDIAL DAMAGE, ATYPICAL MYOPATHY, equine, cardiomyopathy, Cardiac biomarkers, cardiac troponin I

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Chicago
Van Der Vekens, Nicky, Marie-Astrid van Dievoet, Hendrik De Puydt, Annelies Decloedt, Sofie Ven, Dominique De Clercq, Piet Deprez, and Gunther van Loon. 2015. “Analytical Validation of a High-sensitivity Cardiac Troponin T Assay in Horses.” Journal of Veterinary Diagnostic Investigation 27 (4): 504–509.
APA
Van Der Vekens, N., van Dievoet, M.-A., De Puydt, H., Decloedt, A., Ven, S., De Clercq, D., Deprez, P., et al. (2015). Analytical validation of a high-sensitivity cardiac troponin T assay in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 27(4), 504–509.
Vancouver
1.
Van Der Vekens N, van Dievoet M-A, De Puydt H, Decloedt A, Ven S, De Clercq D, et al. Analytical validation of a high-sensitivity cardiac troponin T assay in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2015;27(4):504–9.
MLA
Van Der Vekens, Nicky, Marie-Astrid van Dievoet, Hendrik De Puydt, et al. “Analytical Validation of a High-sensitivity Cardiac Troponin T Assay in Horses.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 27.4 (2015): 504–509. Print.
@article{7193206,
  abstract     = {Although cardiac troponin T (cTnT) assays have been used to detect myocardial damage in horses, a cTnT assay has not been analytically validated, to our knowledge. The aims of this study were to estimate the precision of a high-sensitivity cTnT assay in horses and determine the effect of hemolysis on the measured cTnT concentration. Serum samples from horses were mixed in 3 different pools. Pool 1 consisted of samples from 3 healthy horses, pool 2 from 6 horses with heart failure or atypical myopathy, and pool 3 from 10 horses with atypical myopathy. The within- and between-run coefficients of variation were determined for each pool. Pools 2 and 3 were diluted to estimate linearity. To study the influence of sample hemolysis, serum was collected from 4 horses with a high cTnT concentration, in which hemolysis was mechanically induced. In addition, ethylenediamine tetra-acetic acid blood tubes were collected from 3 other horses, from which hemolysate was prepared and added to plasma at different concentrations. The within- and between-run coefficients of variation of all pools were {\textlangle}10\%, and a good linearity was found. Three out of 4 hemolyzed serum samples had a decreased serum cTnT concentration. Plasma samples with a high hemolysis index showed a negative interference, resulting in a lower cTnT concentration. Results of the high-sensitivity cTnT assay were highly reproducible. Because samples from horses with musculoskeletal damage were included, further studies should test the possible cross-reactivity between troponin T of musculoskeletal and cardiac origin before the assay can be used in equine clinical practice.},
  author       = {Van Der Vekens, Nicky and van Dievoet, Marie-Astrid and De Puydt, Hendrik and Decloedt, Annelies and Ven, Sofie and De Clercq, Dominique and Deprez, Piet and van Loon, Gunther},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {SKELETAL-MUSCLE,I CONCENTRATIONS,HEMOLYSIS,DISEASE,SPECIMENS,INJURY,SERUM,MYOCARDIAL DAMAGE,ATYPICAL MYOPATHY,equine,cardiomyopathy,Cardiac biomarkers,cardiac troponin I},
  language     = {eng},
  number       = {4},
  pages        = {504--509},
  title        = {Analytical validation of a high-sensitivity cardiac troponin T assay in horses},
  url          = {http://dx.doi.org/10.1177/1040638715593601},
  volume       = {27},
  year         = {2015},
}

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