Ghent University Academic Bibliography

Advanced

Genome sequence of the recombinant protein production host Pichia pastoris

Kristof De Schutter UGent, Yao-Cheng Lin UGent, Petra Tiels UGent, Annelies Van Hecke UGent, S Glinka, J Weber-Lehmann, Pierre Rouzé UGent, Yves Van de Peer UGent and Nico Callewaert UGent (2009) NATURE BIOTECHNOLOGY. 27(6). p.561-U104
abstract
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
journal title
NATURE BIOTECHNOLOGY
Nat. Biotechnol.
volume
27
issue
6
pages
561 - U104
Web of Science type
Article
Web of Science id
000266980500026
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
29.495 (2009)
JCR rank
1/150 (2009)
JCR quartile
1 (2009)
ISSN
1087-0156
DOI
10.1038/nbt.1544
language
English
UGent publication?
yes
classification
A1
id
719136
handle
http://hdl.handle.net/1854/LU-719136
date created
2009-07-28 08:45:38
date last changed
2012-06-26 14:31:45
@article{719136,
  abstract     = {The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.},
  author       = {De Schutter, Kristof and Lin, Yao-Cheng and Tiels, Petra and Van Hecke, Annelies and Glinka, S and Weber-Lehmann, J and Rouz{\'e}, Pierre and Van de Peer, Yves and Callewaert, Nico},
  issn         = {1087-0156},
  journal      = {NATURE BIOTECHNOLOGY},
  language     = {eng},
  number       = {6},
  pages        = {561--U104},
  title        = {Genome sequence of the recombinant protein production host Pichia pastoris},
  url          = {http://dx.doi.org/10.1038/nbt.1544},
  volume       = {27},
  year         = {2009},
}

Chicago
De Schutter, Kristof, Yao-Cheng Lin, Petra Tiels, Annelies Van Hecke, S Glinka, J Weber-Lehmann, Pierre Rouzé, Yves Van de Peer, and Nico Callewaert. 2009. “Genome Sequence of the Recombinant Protein Production Host Pichia Pastoris.” Nature Biotechnology 27 (6): 561–U104.
APA
De Schutter, Kristof, Lin, Y.-C., Tiels, P., Van Hecke, A., Glinka, S., Weber-Lehmann, J., Rouzé, P., et al. (2009). Genome sequence of the recombinant protein production host Pichia pastoris. NATURE BIOTECHNOLOGY, 27(6), 561–U104.
Vancouver
1.
De Schutter K, Lin Y-C, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, et al. Genome sequence of the recombinant protein production host Pichia pastoris. NATURE BIOTECHNOLOGY. 2009;27(6):561–U104.
MLA
De Schutter, Kristof, Yao-Cheng Lin, Petra Tiels, et al. “Genome Sequence of the Recombinant Protein Production Host Pichia Pastoris.” NATURE BIOTECHNOLOGY 27.6 (2009): 561–U104. Print.