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Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ

Veerle Fievez UGent, Bruno Vlaeminck UGent, Tom Jenkins, Francis Enjalbert and Michel Doreau (2007) EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 109(8). p.740-756
abstract
Reference techniques to study rumen biohydrogenation (BH) rely on the comparison of intake and duodenal or (ab)omasal flows of polyunsaturated fatty acids (PUFA), whereas the net BH of PUFA to their saturated end-products gives a quantitative measure of accumulating BH intermediates. The current review paper aims at evaluating alternative in vivo, in vitro and in sacco techniques to simulate reference in vivo results of unprotected PUFA sources, as well as strategies for overcoming or manipulating BH. In vivo rumen sampling approaches show potential but require further investigation, whereas in sacco results are inappropriate. In vitro 24-h batch incubations and continuous cultures approach in vivo BH of unprotected C-18 PUFA sources and are useful to assess the pH value at which dissociation of calcium salts of fatty acids occurs, but overestimate the degree of rumen inertness of formaldehyde-treated oil(seeds) and marine products. Batch or continuous cultures provide an accurate estimate (0.6) of the proportion of hydrogenated C-18 PUFA that are converted into their saturated end-product, except for incubations with high levels of fermentable substrate (> 1.0 g/100 mL) in combination with high amounts of C-18 PUFA(> 0.5 mg/mL) or in incubations with EPA and DHA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
in sacco, in vitro, Rumen biohydrogenation, lipolysis, in vivo, DOCOSAHEXAENOIC ACID, DUODENAL FLOW, CONTINUOUS-CULTURE, DAIRY-COWS, MIXED RUMINAL MICROORGANISMS, CONJUGATED LINOLEIC-ACID, UNSATURATED FATTY-ACIDS, MILK-FAT, FISH-OIL, HIGH-CONCENTRATE
journal title
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Eur. J. Lipid Sci. Technol.
volume
109
issue
8
pages
740 - 756
Web of Science type
Article
Web of Science id
000249057900002
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
0.911 (2007)
JCR rank
52/102 (2007)
JCR quartile
3 (2007)
ISSN
1438-7697
DOI
10.1002/ejlt.200700033
language
English
UGent publication?
yes
classification
A1
id
718736
handle
http://hdl.handle.net/1854/LU-718736
date created
2009-07-23 09:09:21
date last changed
2009-09-23 15:41:46
@article{718736,
  abstract     = {Reference techniques to study rumen biohydrogenation (BH) rely on the comparison of intake and duodenal or (ab)omasal flows of polyunsaturated fatty acids (PUFA), whereas the net BH of PUFA to their saturated end-products gives a quantitative measure of accumulating BH intermediates. The current review paper aims at evaluating alternative in vivo, in vitro and in sacco techniques to simulate reference in vivo results of unprotected PUFA sources, as well as strategies for overcoming or manipulating BH. In vivo rumen sampling approaches show potential but require further investigation, whereas in sacco results are inappropriate. In vitro 24-h batch incubations and continuous cultures approach in vivo BH of unprotected C-18 PUFA sources and are useful to assess the pH value at which dissociation of calcium salts of fatty acids occurs, but overestimate the degree of rumen inertness of formaldehyde-treated oil(seeds) and marine products. Batch or continuous cultures provide an accurate estimate (0.6) of the proportion of hydrogenated C-18 PUFA that are converted into their saturated end-product, except for incubations with high levels of fermentable substrate ({\textrangle} 1.0 g/100 mL) in combination with high amounts of C-18 PUFA({\textrangle} 0.5 mg/mL) or in incubations with EPA and DHA.},
  author       = {Fievez, Veerle and Vlaeminck, Bruno and Jenkins, Tom and Enjalbert, Francis and Doreau, Michel},
  issn         = {1438-7697},
  journal      = {EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY},
  keyword      = {in sacco,in vitro,Rumen biohydrogenation,lipolysis,in vivo,DOCOSAHEXAENOIC ACID,DUODENAL FLOW,CONTINUOUS-CULTURE,DAIRY-COWS,MIXED RUMINAL MICROORGANISMS,CONJUGATED LINOLEIC-ACID,UNSATURATED FATTY-ACIDS,MILK-FAT,FISH-OIL,HIGH-CONCENTRATE},
  language     = {eng},
  number       = {8},
  pages        = {740--756},
  title        = {Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ},
  url          = {http://dx.doi.org/10.1002/ejlt.200700033},
  volume       = {109},
  year         = {2007},
}

Chicago
Fievez, Veerle, Bruno Vlaeminck, Tom Jenkins, Francis Enjalbert, and Michel Doreau. 2007. “Assessing Rumen Biohydrogenation and Its Manipulation in Vivo, in Vitro and in Situ.” European Journal of Lipid Science and Technology 109 (8): 740–756.
APA
Fievez, V., Vlaeminck, B., Jenkins, T., Enjalbert, F., & Doreau, M. (2007). Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 109(8), 740–756.
Vancouver
1.
Fievez V, Vlaeminck B, Jenkins T, Enjalbert F, Doreau M. Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 2007;109(8):740–56.
MLA
Fievez, Veerle, Bruno Vlaeminck, Tom Jenkins, et al. “Assessing Rumen Biohydrogenation and Its Manipulation in Vivo, in Vitro and in Situ.” EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 109.8 (2007): 740–756. Print.