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Comparative performance of thin layer agar and Löwenstein-Jensen culture for diagnosis of tuberculosis

(2013) CLINICAL MICROBIOLOGY AND INFECTION. 19(11). p.E502-E508
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Abstract
Sputum smear microscopy for the diagnosis of tuberculosis (TB) is cheap and simple but its sensitivity is low. Culture on Lowenstein-Jensen (LJ) is more sensitive but it takes a long time to yield results. Thin-Layer Agar (TLA) culture was suggested as an equally sensitive and faster alternative. We evaluated the performance of TLA for diagnosing TB in Jogjakarta, Indonesia. People with suspected TB presenting from July 2010 to July 2011 to two chest clinics of the National TB Control Programme network of Jogjakarta were eligible for inclusion. A sputum sample was sent to the Gadjah Mada University microbiology laboratory for concentration, smearing, Ziehl-Neelsen staining and culture on LJ and TLA. Sensitivity of cultures was evaluated against a composite reference standard (any positive culture). Time to detection of Mycobacteria was recorded. Out of 1414 samples, 164 (12%) were smear positive, 99 (7%) were scanty and 1151 (81%) were negative. On TLA and LJ respectively, 168 (12%) and 149 (11%) samples were positive, 72 (5%) and 32 (2%) were contaminated (=0.64; 95% CI 0.59-0.69, p<0.01). Using the reference standard, 196 (14%) TB cases were identified. The sensitivity of TLA was 0.86 (95% CI 0.80-0.90), significantly higher (p0.03) than for LJ (0.76; 95% CI 0.69-0.81). The median time to detection in days was significantly shorter (p<0.01) for TLA (12; 95% CI 11-13) than for LJ (44; 95% CI 43-45). TLA is a rapid and sensitive method for the diagnosis of TB. Implementation studies to evaluate the cost-effectiveness and impact of its introduction into programmatic settings are urgently needed.
Keywords
rapid detection, Culture, sensitivity, Thin Layer Agar, tuberculosis, MYCOBACTERIUM-TUBERCULOSIS, RAPID DETECTION, MICROCOLONY DETECTION, MICROSCOPIC OBSERVATION, RIFAMPICIN RESISTANCE, MIDDLEBROOK 7H11, SPUTUM, ASSAY, METAANALYSIS, PREVALENCE

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Citation

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Chicago
Battaglioli, T, N Rintiswati, Anandi Martin, KR Palupi, G Bernaerts, B Dwihardiani, RA Ahmad, F Matthys, Y Mahendradhata, and Patrick Van Der Stuyft. 2013. “Comparative Performance of Thin Layer Agar and Löwenstein-Jensen Culture for Diagnosis of Tuberculosis.” Clinical Microbiology and Infection 19 (11): E502–E508.
APA
Battaglioli, T., Rintiswati, N., Martin, A., Palupi, K., Bernaerts, G., Dwihardiani, B., Ahmad, R., et al. (2013). Comparative performance of thin layer agar and Löwenstein-Jensen culture for diagnosis of tuberculosis. CLINICAL MICROBIOLOGY AND INFECTION, 19(11), E502–E508.
Vancouver
1.
Battaglioli T, Rintiswati N, Martin A, Palupi K, Bernaerts G, Dwihardiani B, et al. Comparative performance of thin layer agar and Löwenstein-Jensen culture for diagnosis of tuberculosis. CLINICAL MICROBIOLOGY AND INFECTION. 2013;19(11):E502–E508.
MLA
Battaglioli, T, N Rintiswati, Anandi Martin, et al. “Comparative Performance of Thin Layer Agar and Löwenstein-Jensen Culture for Diagnosis of Tuberculosis.” CLINICAL MICROBIOLOGY AND INFECTION 19.11 (2013): E502–E508. Print.
@article{7187345,
  abstract     = {Sputum smear microscopy for the diagnosis of tuberculosis (TB) is cheap and simple but its sensitivity is low. Culture on Lowenstein-Jensen (LJ) is more sensitive but it takes a long time to yield results. Thin-Layer Agar (TLA) culture was suggested as an equally sensitive and faster alternative. We evaluated the performance of TLA for diagnosing TB in Jogjakarta, Indonesia. People with suspected TB presenting from July 2010 to July 2011 to two chest clinics of the National TB Control Programme network of Jogjakarta were eligible for inclusion. A sputum sample was sent to the Gadjah Mada University microbiology laboratory for concentration, smearing, Ziehl-Neelsen staining and culture on LJ and TLA. Sensitivity of cultures was evaluated against a composite reference standard (any positive culture). Time to detection of Mycobacteria was recorded. Out of 1414 samples, 164 (12\%) were smear positive, 99 (7\%) were scanty and 1151 (81\%) were negative. On TLA and LJ respectively, 168 (12\%) and 149 (11\%) samples were positive, 72 (5\%) and 32 (2\%) were contaminated (=0.64; 95\% CI 0.59-0.69, p{\textlangle}0.01). Using the reference standard, 196 (14\%) TB cases were identified. The sensitivity of TLA was 0.86 (95\% CI 0.80-0.90), significantly higher (p0.03) than for LJ (0.76; 95\% CI 0.69-0.81). The median time to detection in days was significantly shorter (p{\textlangle}0.01) for TLA (12; 95\% CI 11-13) than for LJ (44; 95\% CI 43-45). TLA is a rapid and sensitive method for the diagnosis of TB. Implementation studies to evaluate the cost-effectiveness and impact of its introduction into programmatic settings are urgently needed.},
  author       = {Battaglioli, T and Rintiswati, N and Martin, Anandi and Palupi, KR and Bernaerts, G and Dwihardiani, B and Ahmad, RA and Matthys, F and Mahendradhata, Y and Van Der Stuyft, Patrick},
  issn         = {1198-743X},
  journal      = {CLINICAL MICROBIOLOGY AND INFECTION},
  language     = {eng},
  number       = {11},
  pages        = {E502--E508},
  title        = {Comparative performance of thin layer agar and L{\"o}wenstein-Jensen culture for diagnosis of tuberculosis},
  url          = {http://dx.doi.org/10.1111/1469-0691.12265},
  volume       = {19},
  year         = {2013},
}

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