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Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories

Author
Organization
Abstract
The nitrate reductase assay (NRA) was used as an alternative method for detection of resistance to the first-line antituberculous drugs isoniazid, rifampicin, ethambutol and streptomycin. A total of 320 strains of Mycobacterium tuberculosis were studied and the results compared with the proportion method (PM) on Lowenstein-Jensen medium. The mean time to obtain results was 10 days and the overall agreement between the NRA and PM was 98(.)8%. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis strains in low-resource countries.
Keywords
RAPID DETECTION, RIFAMPICIN RESISTANCE, RESAZURIN

Citation

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Chicago
Lemus, D, E Montoro, M Echemendia, Anandi Martin, F Portaels, and Juan Carlos Palomino. 2006. “Nitrate Reductase Assay for Detection of Drug Resistance in Mycobacterium Tuberculosis: Simple and Inexpensive Method for Low-resource Laboratories.” Journal of Medical Microbiology 55 (7): 861–863.
APA
Lemus, D, Montoro, E., Echemendia, M., Martin, A., Portaels, F., & Palomino, J. C. (2006). Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories. JOURNAL OF MEDICAL MICROBIOLOGY, 55(7), 861–863. Presented at the 36th World congress on Lung Health.
Vancouver
1.
Lemus D, Montoro E, Echemendia M, Martin A, Portaels F, Palomino JC. Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories. JOURNAL OF MEDICAL MICROBIOLOGY. 2006;55(7):861–3.
MLA
Lemus, D, E Montoro, M Echemendia, et al. “Nitrate Reductase Assay for Detection of Drug Resistance in Mycobacterium Tuberculosis: Simple and Inexpensive Method for Low-resource Laboratories.” JOURNAL OF MEDICAL MICROBIOLOGY 55.7 (2006): 861–863. Print.
@article{7187011,
  abstract     = {The nitrate reductase assay (NRA) was used as an alternative method for detection of resistance to the first-line antituberculous drugs isoniazid, rifampicin, ethambutol and streptomycin. A total of 320 strains of Mycobacterium tuberculosis were studied and the results compared with the proportion method (PM) on Lowenstein-Jensen medium. The mean time to obtain results was 10 days and the overall agreement between the NRA and PM was 98(.)8\%. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis strains in low-resource countries.},
  author       = {Lemus, D and Montoro, E and Echemendia, M and Martin, Anandi and Portaels, F and Palomino, Juan Carlos},
  issn         = {0022-2615},
  journal      = {JOURNAL OF MEDICAL MICROBIOLOGY},
  language     = {eng},
  location     = {Paris, France},
  number       = {7},
  pages        = {861--863},
  title        = {Nitrate reductase assay for detection of drug resistance in Mycobacterium tuberculosis: simple and inexpensive method for low-resource laboratories},
  url          = {http://dx.doi.org/10.1099/jmm.0.46540-0},
  volume       = {55},
  year         = {2006},
}

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