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Protective effect of resveratrol and quercetin on in vitro-induced diabetic mouse corpus cavernosum

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Abstract
Background: Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Resveratrol and quercetin are red wine polyphenols with known beneficial cardiovascular properties, including an antioxidant capacity. This study evaluated whether resveratrol and/or quercetin could prevent in vitro-induced diabetic changes in neurogenic and vascular relaxant responses of mouse arteries and corpora cavernosa. Methods: Isometric tension of isolated aorta, mesenteric arteries and corpora cavernosa was measured using organ bath systems. Diabetic conditions were mimicked in vitro by co-incubating the tissues for 2 h with high glucose (HG, 30 mM) and MGO (120 mu M). Results: The presence of HG and MGO significantly blunted acetylcholine (Ach)-induced relaxations in corpora cavernosa and mesenteric arteries but not in aorta. Electrical field stimulated (EFS) responses of corpora cavernosa were also significantly inhibited by these diabetic conditions. In corpora cavernosa 2 h co-incubation with resveratrol (30 mu M) or quercetin (30 mu M) significantly attenuated HG and MGO-induced deficits in Ach- and EFS-responses. Conclusions: Our study demonstrates that in mouse arteries, HG and MGO rather affect endothelium derived hyperpolarizing factor-mediated than nitric oxide (NO)-mediated relaxations. In corpora cavernosa HG and MGO interfere with NO release. Resveratrol and quercetin protect mouse corpora cavernosa from diabetic-induced damage to NO-mediated relaxant responses. This might rely on their antioxidant capacity.
Keywords
ANTIOXIDANT, GLUCOSE, RAT AORTA, METHYLGLYOXAL, OXIDATIVE STRESS, MESENTERIC-ARTERIES, ERECTILE DYSFUNCTION, ENDOTHELIUM-DEPENDENT RELAXATION, ALPHA-LIPOIC ACID, Erectile dysfunction, Diabetes, Quercetin, Resveratrol, MICE

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MLA
Boydens, Charlotte, et al. “Protective Effect of Resveratrol and Quercetin on in Vitro-Induced Diabetic Mouse Corpus Cavernosum.” CARDIOVASCULAR DIABETOLOGY, vol. 15, 2016, doi:10.1186/s12933-016-0366-9.
APA
Boydens, C., Pauwels, B., Vanden Daele, L., & Van de Voorde, J. (2016). Protective effect of resveratrol and quercetin on in vitro-induced diabetic mouse corpus cavernosum. CARDIOVASCULAR DIABETOLOGY, 15. https://doi.org/10.1186/s12933-016-0366-9
Chicago author-date
Boydens, Charlotte, Bart Pauwels, Laura Vanden Daele, and Johan Van de Voorde. 2016. “Protective Effect of Resveratrol and Quercetin on in Vitro-Induced Diabetic Mouse Corpus Cavernosum.” CARDIOVASCULAR DIABETOLOGY 15. https://doi.org/10.1186/s12933-016-0366-9.
Chicago author-date (all authors)
Boydens, Charlotte, Bart Pauwels, Laura Vanden Daele, and Johan Van de Voorde. 2016. “Protective Effect of Resveratrol and Quercetin on in Vitro-Induced Diabetic Mouse Corpus Cavernosum.” CARDIOVASCULAR DIABETOLOGY 15. doi:10.1186/s12933-016-0366-9.
Vancouver
1.
Boydens C, Pauwels B, Vanden Daele L, Van de Voorde J. Protective effect of resveratrol and quercetin on in vitro-induced diabetic mouse corpus cavernosum. CARDIOVASCULAR DIABETOLOGY. 2016;15.
IEEE
[1]
C. Boydens, B. Pauwels, L. Vanden Daele, and J. Van de Voorde, “Protective effect of resveratrol and quercetin on in vitro-induced diabetic mouse corpus cavernosum,” CARDIOVASCULAR DIABETOLOGY, vol. 15, 2016.
@article{7185051,
  abstract     = {{Background: Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Resveratrol and quercetin are red wine polyphenols with known beneficial cardiovascular properties, including an antioxidant capacity. This study evaluated whether resveratrol and/or quercetin could prevent in vitro-induced diabetic changes in neurogenic and vascular relaxant responses of mouse arteries and corpora cavernosa. 
Methods: Isometric tension of isolated aorta, mesenteric arteries and corpora cavernosa was measured using organ bath systems. Diabetic conditions were mimicked in vitro by co-incubating the tissues for 2 h with high glucose (HG, 30 mM) and MGO (120 mu M). 
Results: The presence of HG and MGO significantly blunted acetylcholine (Ach)-induced relaxations in corpora cavernosa and mesenteric arteries but not in aorta. Electrical field stimulated (EFS) responses of corpora cavernosa were also significantly inhibited by these diabetic conditions. In corpora cavernosa 2 h co-incubation with resveratrol (30 mu M) or quercetin (30 mu M) significantly attenuated HG and MGO-induced deficits in Ach- and EFS-responses. 
Conclusions: Our study demonstrates that in mouse arteries, HG and MGO rather affect endothelium derived hyperpolarizing factor-mediated than nitric oxide (NO)-mediated relaxations. In corpora cavernosa HG and MGO interfere with NO release. Resveratrol and quercetin protect mouse corpora cavernosa from diabetic-induced damage to NO-mediated relaxant responses. This might rely on their antioxidant capacity.}},
  articleno    = {{46}},
  author       = {{Boydens, Charlotte and Pauwels, Bart and Vanden Daele, Laura and Van de Voorde, Johan}},
  issn         = {{1475-2840}},
  journal      = {{CARDIOVASCULAR DIABETOLOGY}},
  keywords     = {{ANTIOXIDANT,GLUCOSE,RAT AORTA,METHYLGLYOXAL,OXIDATIVE STRESS,MESENTERIC-ARTERIES,ERECTILE DYSFUNCTION,ENDOTHELIUM-DEPENDENT RELAXATION,ALPHA-LIPOIC ACID,Erectile dysfunction,Diabetes,Quercetin,Resveratrol,MICE}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Protective effect of resveratrol and quercetin on in vitro-induced diabetic mouse corpus cavernosum}},
  url          = {{http://doi.org/10.1186/s12933-016-0366-9}},
  volume       = {{15}},
  year         = {{2016}},
}

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