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Electron tomography, three-dimensional Fourier analysis and colour prediction of a three-dimensional amorphous biophotonic nanostructure

(2009) JOURNAL OF THE ROYAL SOCIETY INTERFACE. 6(suppl. 2). p.S213-S220
Author
Organization
Abstract
Organismal colour can be created by selective absorption of light by pigments or light scattering by photonic nanostructures. Photonic nanostructures may vary in refractive index over one, two or three dimensions and may be periodic over large spatial scales or amorphous with short-range order. Theoretical optical analysis of three-dimensional amorphous nanostructures has been challenging because these structures are difficult to describe accurately from conventional two-dimensional electron microscopy alone. Intermediate voltage electron microscopy ( IVEM) with tomographic reconstruction adds three-dimensional data by using a high-power electron beam to penetrate and image sections of material sufficiently thick to contain a significant portion of the structure. Here, we use IVEM tomography to characterize a non-iridescent, three-dimensional biophotonic nanostructure: the spongy medullary layer from eastern bluebird Sialia sialis feather barbs. Tomography and three-dimensional Fourier analysis reveal that it is an amorphous, interconnected bicontinuous matrix that is appropriately ordered at local spatial scales in all three dimensions to coherently scatter light. The predicted reflectance spectra from the three-dimensional Fourier analysis are more precise than those predicted by previous two-dimensional Fourier analysis of transmission electron microscopy sections. These results highlight the usefulness, and obstacles, of tomography in the description and analysis of three-dimensional photonic structures.
Keywords
biophotonics, structural colour, tomography, Fourier analysis, feathers, COHERENT-LIGHT SCATTERING, DERMAL COLLAGEN ARRAYS, STRUCTURAL COLORATION, FEATHER BARBS, COMPUTER VISUALIZATION, CONVERGENT EVOLUTION, BUTTERFLIES, SYSTEM, SKIN, EYE

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Chicago
Shawkey, Matthew, Vinodkumar Saranathan, Hildur Palsdottir, John Crum, Mark H Ellisman, Manfred Auer, and Richard O Prum. 2009. “Electron Tomography, Three-dimensional Fourier Analysis and Colour Prediction of a Three-dimensional Amorphous Biophotonic Nanostructure.” Journal of the Royal Society Interface 6 (suppl. 2): S213–S220.
APA
Shawkey, M., Saranathan, V., Palsdottir, H., Crum, J., Ellisman, M. H., Auer, M., & Prum, R. O. (2009). Electron tomography, three-dimensional Fourier analysis and colour prediction of a three-dimensional amorphous biophotonic nanostructure. JOURNAL OF THE ROYAL SOCIETY INTERFACE, 6(suppl. 2), S213–S220. Presented at the Conference on Iridescence: More than meets the eye.
Vancouver
1.
Shawkey M, Saranathan V, Palsdottir H, Crum J, Ellisman MH, Auer M, et al. Electron tomography, three-dimensional Fourier analysis and colour prediction of a three-dimensional amorphous biophotonic nanostructure. JOURNAL OF THE ROYAL SOCIETY INTERFACE. 2009;6(suppl. 2):S213–S220.
MLA
Shawkey, Matthew, Vinodkumar Saranathan, Hildur Palsdottir, et al. “Electron Tomography, Three-dimensional Fourier Analysis and Colour Prediction of a Three-dimensional Amorphous Biophotonic Nanostructure.” JOURNAL OF THE ROYAL SOCIETY INTERFACE 6.suppl. 2 (2009): S213–S220. Print.
@article{7176738,
  abstract     = {Organismal colour can be created by selective absorption of light by pigments or light scattering by photonic nanostructures. Photonic nanostructures may vary in refractive index over one, two or three dimensions and may be periodic over large spatial scales or amorphous with short-range order. Theoretical optical analysis of three-dimensional amorphous nanostructures has been challenging because these structures are difficult to describe accurately from conventional two-dimensional electron microscopy alone. Intermediate voltage electron microscopy ( IVEM) with tomographic reconstruction adds three-dimensional data by using a high-power electron beam to penetrate and image sections of material sufficiently thick to contain a significant portion of the structure. Here, we use IVEM tomography to characterize a non-iridescent, three-dimensional biophotonic nanostructure: the spongy medullary layer from eastern bluebird Sialia sialis feather barbs. Tomography and three-dimensional Fourier analysis reveal that it is an amorphous, interconnected bicontinuous matrix that is appropriately ordered at local spatial scales in all three dimensions to coherently scatter light. The predicted reflectance spectra from the three-dimensional Fourier analysis are more precise than those predicted by previous two-dimensional Fourier analysis of transmission electron microscopy sections. These results highlight the usefulness, and obstacles, of tomography in the description and analysis of three-dimensional photonic structures.},
  author       = {Shawkey, Matthew and Saranathan, Vinodkumar and Palsdottir, Hildur and Crum, John and Ellisman, Mark H and Auer, Manfred and Prum, Richard O},
  issn         = {1742-5689},
  journal      = {JOURNAL OF THE ROYAL SOCIETY INTERFACE},
  language     = {eng},
  location     = {Tempe, AZ, USA},
  number       = {suppl. 2},
  pages        = {S213--S220},
  title        = {Electron tomography, three-dimensional Fourier analysis and colour prediction of a three-dimensional amorphous biophotonic nanostructure},
  url          = {http://dx.doi.org/10.1098/rsif.2008.0374.focus},
  volume       = {6},
  year         = {2009},
}

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