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Membrane protein expression and analysis in yeast

Katrien Claes (UGent) , Mouna Guerfal (UGent) and Nico Callewaert (UGent)
(2015) METHODS IN ENZYMOLOGY. 556. p.123-140
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Abstract
This protocol describes the expression and analysis of membrane proteins produced in yeast, as illustrated with Yarrowia lipolytica and Pichia pastoris. Step by step, we explain how to generate a yeast strain expressing the membrane protein of interest, how to prepare a membrane protein sample from yeast, and how to analyze the expression levels using SDS-PAGE and Western blotting. In the final section, we describe how to perform a radioligand binding assay, which quantifies the amount of the protein folded in a ligand-binding competent state.

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MLA
Claes, Katrien, et al. “Membrane Protein Expression and Analysis in Yeast.” METHODS IN ENZYMOLOGY, edited by Arun K Shukla, vol. 556, 2015, pp. 123–40, doi:10.1016/bs.mie.2014.12.010.
APA
Claes, K., Guerfal, M., & Callewaert, N. (2015). Membrane protein expression and analysis in yeast. METHODS IN ENZYMOLOGY, 556, 123–140. https://doi.org/10.1016/bs.mie.2014.12.010
Chicago author-date
Claes, Katrien, Mouna Guerfal, and Nico Callewaert. 2015. “Membrane Protein Expression and Analysis in Yeast.” Edited by Arun K Shukla. METHODS IN ENZYMOLOGY 556: 123–40. https://doi.org/10.1016/bs.mie.2014.12.010.
Chicago author-date (all authors)
Claes, Katrien, Mouna Guerfal, and Nico Callewaert. 2015. “Membrane Protein Expression and Analysis in Yeast.” Ed by. Arun K Shukla. METHODS IN ENZYMOLOGY 556: 123–140. doi:10.1016/bs.mie.2014.12.010.
Vancouver
1.
Claes K, Guerfal M, Callewaert N. Membrane protein expression and analysis in yeast. Shukla AK, editor. METHODS IN ENZYMOLOGY. 2015;556:123–40.
IEEE
[1]
K. Claes, M. Guerfal, and N. Callewaert, “Membrane protein expression and analysis in yeast,” METHODS IN ENZYMOLOGY, vol. 556, pp. 123–140, 2015.
@article{7175081,
  abstract     = {{This protocol describes the expression and analysis of membrane proteins produced in yeast, as illustrated with Yarrowia lipolytica and Pichia pastoris. Step by step, we explain how to generate a yeast strain expressing the membrane protein of interest, how to prepare a membrane protein sample from yeast, and how to analyze the expression levels using SDS-PAGE and Western blotting. In the final section, we describe how to perform a radioligand binding assay, which quantifies the amount of the protein folded in a ligand-binding competent state.}},
  author       = {{Claes, Katrien and Guerfal, Mouna and Callewaert, Nico}},
  editor       = {{Shukla, Arun K}},
  isbn         = {{9780128015216}},
  issn         = {{0076-6879}},
  journal      = {{METHODS IN ENZYMOLOGY}},
  language     = {{eng}},
  pages        = {{123--140}},
  title        = {{Membrane protein expression and analysis in yeast}},
  url          = {{http://dx.doi.org/10.1016/bs.mie.2014.12.010}},
  volume       = {{556}},
  year         = {{2015}},
}

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