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PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root

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Abstract
In plants, the generation of new cell types and tissues depends on coordinated and oriented formative cell divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part of a mechanism controlling formative cell divisions in the Arabidopsis root. Despite its important role in plant development, very little is known about the molecular mechanism with which ACR4 is affiliated and its network of interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely regulators of formative cell divisions and that could pave the way to unraveling the molecular basis behind ACR4-mediated signaling. We identified PROTEIN PHOSPHATASE 2A-3 (PP2A-3), a catalytic subunit of PP2A holoenzymes, as a previously unidentified regulator of formative cell divisions and as one of the first described substrates of ACR4. Our in vitro data argue for the existence of a tight posttranslational regulation in the associated biochemical network through reciprocal regulation between ACR4 and PP2A-3 at the phosphorylation level.
Keywords
PPP FAMILY, GENE, STRESS-RESPONSE, SERINE/THREONINE PHOSPHATASES, RECEPTOR-LIKE KINASE, TANDEM AFFINITY PURIFICATION, PROTEIN PHOSPHATASE 2A, phosphatase, kinase, phosphorylation, columella, stem cells, DIFFERENTIATION, THALIANA, PLANTS

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Chicago
Yue, Kun, Priyanka Sandal, Elisabeth L Williams, Evan Murphy, Elisabeth Stes, Natalia Nikonorova, Priya Ramakrishna, et al. 2016. “PP2A-3 Interacts with ACR4 and Regulates Formative Cell Division in the Arabidopsis Root.” Proceedings of the National Academy of Sciences of the United States of America 113 (5): 1447–1452.
APA
Yue, K., Sandal, P., Williams, E. L., Murphy, E., Stes, E., Nikonorova, N., Ramakrishna, P., et al. (2016). PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(5), 1447–1452.
Vancouver
1.
Yue K, Sandal P, Williams EL, Murphy E, Stes E, Nikonorova N, et al. PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2016;113(5):1447–52.
MLA
Yue, Kun, Priyanka Sandal, Elisabeth L Williams, et al. “PP2A-3 Interacts with ACR4 and Regulates Formative Cell Division in the Arabidopsis Root.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.5 (2016): 1447–1452. Print.
@article{7170090,
  abstract     = {In plants, the generation of new cell types and tissues depends on coordinated and oriented formative cell divisions. The plasma membrane-localized receptor kinase ARABIDOPSIS CRINKLY 4 (ACR4) is part of a mechanism controlling formative cell divisions in the Arabidopsis root. Despite its important role in plant development, very little is known about the molecular mechanism with which ACR4 is affiliated and its network of interactions. Here, we used various complementary proteomic approaches to identify ACR4-interacting protein candidates that are likely regulators of formative cell divisions and that could pave the way to unraveling the molecular basis behind ACR4-mediated signaling. We identified PROTEIN PHOSPHATASE 2A-3 (PP2A-3), a catalytic subunit of PP2A holoenzymes, as a previously unidentified regulator of formative cell divisions and as one of the first described substrates of ACR4. Our in vitro data argue for the existence of a tight posttranslational regulation in the associated biochemical network through reciprocal regulation between ACR4 and PP2A-3 at the phosphorylation level.},
  author       = {Yue, Kun and Sandal, Priyanka and Williams, Elisabeth L and Murphy, Evan and Stes, Elisabeth and Nikonorova, Natalia and Ramakrishna, Priya and Czyzewicz, Nathan and Montero Morales, Laura and Kumpf, Robert and Lin, Zhefeng and Van De Cotte, Brigitte and Iqbal, Mudassar and Van Bel, Michiel and Van De Slijke, Eveline and Meyer, Matthew R and Gadeyne, Astrid and Zipfel, Cyril and De Jaeger, Geert and Van Montagu, Marc and Van Damme, Dani{\"e}l and Gevaert, Kris and Rao, A Gururaj and Beeckman, Tom and De Smet, Ive},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {PPP FAMILY,GENE,STRESS-RESPONSE,SERINE/THREONINE PHOSPHATASES,RECEPTOR-LIKE KINASE,TANDEM AFFINITY PURIFICATION,PROTEIN PHOSPHATASE 2A,phosphatase,kinase,phosphorylation,columella,stem cells,DIFFERENTIATION,THALIANA,PLANTS},
  language     = {eng},
  number       = {5},
  pages        = {1447--1452},
  title        = {PP2A-3 interacts with ACR4 and regulates formative cell division in the Arabidopsis root},
  url          = {http://dx.doi.org/10.1073/pnas.1525122113},
  volume       = {113},
  year         = {2016},
}

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