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Overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize

Wannes Voorend, Hilde Nelissen UGent, Ruben Vanholme UGent, Alex De Vliegher, Frank Van Breusegem UGent, Wout Boerjan UGent, Isabel Roldàn-Ruiz UGent, Hilde Muylle and Dirk Inzé UGent (2016) PLANT BIOTECHNOLOGY JOURNAL. 14(3). p.997-1007
abstract
Increased biomass yield and quality are of great importance for the improvement of feedstock for the biorefinery. For the production of bioethanol, both stem biomass yield and the conversion efficiency of the polysaccharides in the cell wall to fermentable sugars are of relevance. Increasing the endogenous levels of gibberellic acid (GA) by ectopic expression of GA20-OXIDASE1 (GA20-OX1), the rate-limiting step in GA biosynthesis, is known to affect cell division and cell expansion, resulting in larger plants and organs in several plant species. In this study, we examined biomass yield and quality traits of maize plants overexpressing GA20-OX1 (GA20-OX1). GA20-OX1 plants accumulated more vegetative biomass than control plants in greenhouse experiments, but not consistently over two years of field trials. The stems of these plants were longer but also more slender. Investigation of GA20-OX1 biomass quality using biochemical analyses showed the presence of more cellulose, lignin and cell wall residue. Cell wall analysis as well as expression analysis of lignin biosynthetic genes in developing stems revealed that cellulose and lignin were deposited earlier in development. Pretreatment of GA20-OX1 biomass with NaOH resulted in a higher saccharification efficiency per unit of dry weight, in agreement with the higher cellulose content. On the other hand, the cellulose-to-glucose conversion was slower upon HCl or hot-water pretreatment, presumably due to the higher lignin content. This study showed that biomass yield and quality traits can be interconnected, which is important for the development of future breeding strategies to improve lignocellulosic feedstock for bioethanol production.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
bioenergy, gibberellic acid, lignocellulose, maize, plant growth, crop yield, CELL-WALL DIGESTIBILITY, ZEA-MAYS L., PRIMARY PHLOEM FIBERS, GIBBERELLIN METABOLISM, LIGNIN BIOSYNTHESIS, GREEN-REVOLUTION, BIOENERGY PRODUCTION, BIOFUEL PRODUCTION, SIGNALING GENES, REDUCED LIGNIN
journal title
PLANT BIOTECHNOLOGY JOURNAL
Plant Biotechnol. J.
volume
14
issue
3
pages
997 - 1007
Web of Science type
Article
Web of Science id
000371224700016
JCR category
PLANT SCIENCES
JCR impact factor
7.443 (2016)
JCR rank
7/211 (2016)
JCR quartile
1 (2016)
ISSN
1467-7644
DOI
10.1111/pbi.12458
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
7170054
handle
http://hdl.handle.net/1854/LU-7170054
date created
2016-03-31 10:22:20
date last changed
2017-03-02 09:56:22
@article{7170054,
  abstract     = {Increased biomass yield and quality are of great importance for the improvement of feedstock for the biorefinery. For the production of bioethanol, both stem biomass yield and the conversion efficiency of the polysaccharides in the cell wall to fermentable sugars are of relevance. Increasing the endogenous levels of gibberellic acid (GA) by ectopic expression of GA20-OXIDASE1 (GA20-OX1), the rate-limiting step in GA biosynthesis, is known to affect cell division and cell expansion, resulting in larger plants and organs in several plant species. In this study, we examined biomass yield and quality traits of maize plants overexpressing GA20-OX1 (GA20-OX1). GA20-OX1 plants accumulated more vegetative biomass than control plants in greenhouse experiments, but not consistently over two years of field trials. The stems of these plants were longer but also more slender. Investigation of GA20-OX1 biomass quality using biochemical analyses showed the presence of more cellulose, lignin and cell wall residue. Cell wall analysis as well as expression analysis of lignin biosynthetic genes in developing stems revealed that cellulose and lignin were deposited earlier in development. Pretreatment of GA20-OX1 biomass with NaOH resulted in a higher saccharification efficiency per unit of dry weight, in agreement with the higher cellulose content. On the other hand, the cellulose-to-glucose conversion was slower upon HCl or hot-water pretreatment, presumably due to the higher lignin content. This study showed that biomass yield and quality traits can be interconnected, which is important for the development of future breeding strategies to improve lignocellulosic feedstock for bioethanol production.},
  author       = {Voorend, Wannes and Nelissen, Hilde and Vanholme, Ruben and De Vliegher, Alex and Van Breusegem, Frank and Boerjan, Wout and Rold{\`a}n-Ruiz, Isabel and Muylle, Hilde and Inz{\'e}, Dirk},
  issn         = {1467-7644},
  journal      = {PLANT BIOTECHNOLOGY JOURNAL},
  keyword      = {bioenergy,gibberellic acid,lignocellulose,maize,plant growth,crop yield,CELL-WALL DIGESTIBILITY,ZEA-MAYS L.,PRIMARY PHLOEM FIBERS,GIBBERELLIN METABOLISM,LIGNIN BIOSYNTHESIS,GREEN-REVOLUTION,BIOENERGY PRODUCTION,BIOFUEL PRODUCTION,SIGNALING GENES,REDUCED LIGNIN},
  language     = {eng},
  number       = {3},
  pages        = {997--1007},
  title        = {Overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize},
  url          = {http://dx.doi.org/10.1111/pbi.12458},
  volume       = {14},
  year         = {2016},
}

Chicago
Voorend, Wannes, Hilde Nelissen, Ruben Vanholme, Alex De Vliegher, Frank Van Breusegem, Wout Boerjan, Isabel Roldàn-Ruiz, Hilde Muylle, and Dirk Inzé. 2016. “Overexpression of GA20-OXIDASE1 Impacts Plant Height, Biomass Allocation and Saccharification Efficiency in Maize.” Plant Biotechnology Journal 14 (3): 997–1007.
APA
Voorend, W., Nelissen, H., Vanholme, R., De Vliegher, A., Van Breusegem, F., Boerjan, W., Roldàn-Ruiz, I., et al. (2016). Overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize. PLANT BIOTECHNOLOGY JOURNAL, 14(3), 997–1007.
Vancouver
1.
Voorend W, Nelissen H, Vanholme R, De Vliegher A, Van Breusegem F, Boerjan W, et al. Overexpression of GA20-OXIDASE1 impacts plant height, biomass allocation and saccharification efficiency in maize. PLANT BIOTECHNOLOGY JOURNAL. 2016;14(3):997–1007.
MLA
Voorend, Wannes, Hilde Nelissen, Ruben Vanholme, et al. “Overexpression of GA20-OXIDASE1 Impacts Plant Height, Biomass Allocation and Saccharification Efficiency in Maize.” PLANT BIOTECHNOLOGY JOURNAL 14.3 (2016): 997–1007. Print.