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Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

(2016) PLANT BIOTECHNOLOGY JOURNAL. 14(1). p.85-96
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Abstract
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert beta-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. beta-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.
Keywords
TAXOL BIOSYNTHESIS, SECONDARY METABOLISM, FUNCTIONAL GENOMICS, BIOTECHNOLOGICAL PRODUCTION, METHYL JASMONATE, SUSPENSION-CULTURES, BACCATIN-III PRODUCTION, TRANSIENT-EXPRESSION SYSTEM, cell cultures, Taxus baccata, Taxol, beta-phenylalanine, methyl jasmonate, CoA ligase, cDNA-AFLP, GENE FAMILY, PACLITAXEL

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MLA
Ramírez-Estrada, Karla et al. “Transcript Profiling of Jasmonate-elicited Taxus Cells Reveals a β-phenylalanine-CoA Ligase.” PLANT BIOTECHNOLOGY JOURNAL 14.1 (2016): 85–96. Print.
APA
Ramírez-Estrada, K., Altabella, T., Onrubia, M., Moyano, E., Notredame, C., Osuna, L., Vanden Bossche, R., et al. (2016). Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase. PLANT BIOTECHNOLOGY JOURNAL, 14(1), 85–96.
Chicago author-date
Ramírez-Estrada, Karla, Teresa Altabella, Miriam Onrubia, Elisabeth Moyano, Cedric Notredame, Lidia Osuna, Robin Vanden Bossche, Alain Goossens, Rosa M Cusido, and Javier Palazon. 2016. “Transcript Profiling of Jasmonate-elicited Taxus Cells Reveals a β-phenylalanine-CoA Ligase.” Plant Biotechnology Journal 14 (1): 85–96.
Chicago author-date (all authors)
Ramírez-Estrada, Karla, Teresa Altabella, Miriam Onrubia, Elisabeth Moyano, Cedric Notredame, Lidia Osuna, Robin Vanden Bossche, Alain Goossens, Rosa M Cusido, and Javier Palazon. 2016. “Transcript Profiling of Jasmonate-elicited Taxus Cells Reveals a β-phenylalanine-CoA Ligase.” Plant Biotechnology Journal 14 (1): 85–96.
Vancouver
1.
Ramírez-Estrada K, Altabella T, Onrubia M, Moyano E, Notredame C, Osuna L, et al. Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase. PLANT BIOTECHNOLOGY JOURNAL. 2016;14(1):85–96.
IEEE
[1]
K. Ramírez-Estrada et al., “Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase,” PLANT BIOTECHNOLOGY JOURNAL, vol. 14, no. 1, pp. 85–96, 2016.
@article{7169989,
  abstract     = {Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert beta-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. beta-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.},
  author       = {Ramírez-Estrada, Karla and Altabella, Teresa and Onrubia, Miriam and Moyano, Elisabeth and Notredame, Cedric and Osuna, Lidia and Vanden Bossche, Robin and Goossens, Alain and Cusido, Rosa M and Palazon, Javier},
  issn         = {1467-7644},
  journal      = {PLANT BIOTECHNOLOGY JOURNAL},
  keywords     = {TAXOL BIOSYNTHESIS,SECONDARY METABOLISM,FUNCTIONAL GENOMICS,BIOTECHNOLOGICAL PRODUCTION,METHYL JASMONATE,SUSPENSION-CULTURES,BACCATIN-III PRODUCTION,TRANSIENT-EXPRESSION SYSTEM,cell cultures,Taxus baccata,Taxol,beta-phenylalanine,methyl jasmonate,CoA ligase,cDNA-AFLP,GENE FAMILY,PACLITAXEL},
  language     = {eng},
  number       = {1},
  pages        = {85--96},
  title        = {Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase},
  url          = {http://dx.doi.org/10.1111/pbi.12359},
  volume       = {14},
  year         = {2016},
}

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