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Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase

Karla Ramírez-Estrada, Teresa Altabella, Miriam Onrubia, Elisabeth Moyano, Cedric Notredame, Lidia Osuna, Robin Vanden Bossche UGent, Alain Goossens UGent, Rosa M Cusido and Javier Palazon (2016) PLANT BIOTECHNOLOGY JOURNAL. 14(1). p.85-96
abstract
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert beta-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. beta-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.
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author
organization
alternative title
Transcript profiling of jasmonate-elicited Taxus cells reveals a beta-phenylalanine-CoA ligase
year
type
journalArticle (original)
publication status
published
subject
keyword
TAXOL BIOSYNTHESIS, SECONDARY METABOLISM, FUNCTIONAL GENOMICS, BIOTECHNOLOGICAL PRODUCTION, METHYL JASMONATE, SUSPENSION-CULTURES, BACCATIN-III PRODUCTION, TRANSIENT-EXPRESSION SYSTEM, cell cultures, Taxus baccata, Taxol, beta-phenylalanine, methyl jasmonate, CoA ligase, cDNA-AFLP, GENE FAMILY, PACLITAXEL
journal title
PLANT BIOTECHNOLOGY JOURNAL
Plant Biotechnol. J.
volume
14
issue
1
pages
85 - 96
Web of Science type
Article
Web of Science id
000369285700010
ISSN
1467-7644
DOI
10.1111/pbi.12359
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7169989
handle
http://hdl.handle.net/1854/LU-7169989
date created
2016-03-31 09:50:55
date last changed
2016-12-19 15:47:33
@article{7169989,
  abstract     = {Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert beta-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. beta-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.},
  author       = {Ram{\'i}rez-Estrada, Karla and Altabella, Teresa and Onrubia, Miriam and Moyano, Elisabeth and Notredame, Cedric and Osuna, Lidia and Vanden Bossche, Robin and Goossens, Alain and Cusido, Rosa M and Palazon, Javier},
  issn         = {1467-7644},
  journal      = {PLANT BIOTECHNOLOGY JOURNAL},
  keyword      = {TAXOL BIOSYNTHESIS,SECONDARY METABOLISM,FUNCTIONAL GENOMICS,BIOTECHNOLOGICAL PRODUCTION,METHYL JASMONATE,SUSPENSION-CULTURES,BACCATIN-III PRODUCTION,TRANSIENT-EXPRESSION SYSTEM,cell cultures,Taxus baccata,Taxol,beta-phenylalanine,methyl jasmonate,CoA ligase,cDNA-AFLP,GENE FAMILY,PACLITAXEL},
  language     = {eng},
  number       = {1},
  pages        = {85--96},
  title        = {Transcript profiling of jasmonate-elicited Taxus cells reveals a \ensuremath{\beta}-phenylalanine-CoA ligase},
  url          = {http://dx.doi.org/10.1111/pbi.12359},
  volume       = {14},
  year         = {2016},
}

Chicago
Ramírez-Estrada, Karla, Teresa Altabella, Miriam Onrubia, Elisabeth Moyano, Cedric Notredame, Lidia Osuna, Robin Vanden Bossche, Alain Goossens, Rosa M Cusido, and Javier Palazon. 2016. “Transcript Profiling of Jasmonate-elicited Taxus Cells Reveals a β-phenylalanine-CoA Ligase.” Plant Biotechnology Journal 14 (1): 85–96.
APA
Ramírez-Estrada, K., Altabella, T., Onrubia, M., Moyano, E., Notredame, C., Osuna, L., Vanden Bossche, R., et al. (2016). Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase. PLANT BIOTECHNOLOGY JOURNAL, 14(1), 85–96.
Vancouver
1.
Ramírez-Estrada K, Altabella T, Onrubia M, Moyano E, Notredame C, Osuna L, et al. Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase. PLANT BIOTECHNOLOGY JOURNAL. 2016;14(1):85–96.
MLA
Ramírez-Estrada, Karla, Teresa Altabella, Miriam Onrubia, et al. “Transcript Profiling of Jasmonate-elicited Taxus Cells Reveals a β-phenylalanine-CoA Ligase.” PLANT BIOTECHNOLOGY JOURNAL 14.1 (2016): 85–96. Print.