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Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation

(2016) GENES & DEVELOPMENT. 30(4). p.471-483
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Abstract
To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with a preserved proliferation capacity. The root pericycle represents a unique tissue with conditional meristematic activity, and its tight control determines initiation of lateral organs. Here we show that the meristematic activity of the pericycle is constrained by the interaction with the adjacent endodermis. Release of these restraints by elimination of endodermal cells by single-cell ablation triggers the pericycle to re-enter the cell cycle. We found that endodermis removal substitutes for the phytohormone auxin-dependent initiation of the pericycle meristematic activity. However, auxin is indispensable to steer the cell division plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally distinct role for auxin during lateral root initiation. In the endodermis, auxin releases constraints arising from cell-to-cell interactions that compromise the pericycle meristematic activity, whereas, in the pericycle, auxin defines the orientation of the cell division plane to initiate lateral roots.
Keywords
BIOSYNTHESIS, GENE, TRANSPORT, PERICYCLE, PLANT-CELLS, PROTEINS, EPIDERMAL-CELLS, MECHANICAL-STRESS, DEVELOPMENTAL WINDOW, ARABIDOPSIS-THALIANA, auxin, meristem proliferation activity, mechanical forces, lateral root organogenesis

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Chicago
Marhavy, Peter, Juan Carlos Montesinos, Anas Abuzeineh, Daniël Van Damme, Joop EM Vermeer, Jérôme Duclercq, Hana Rakusova, et al. 2016. “Targeted Cell Elimination Reveals an Auxin-guided Biphasic Mode of Lateral Root Initiation.” Genes & Development 30 (4): 471–483.
APA
Marhavy, P., Montesinos, J. C., Abuzeineh, A., Van Damme, D., Vermeer, J. E., Duclercq, J., Rakusova, H., et al. (2016). Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation. GENES & DEVELOPMENT, 30(4), 471–483.
Vancouver
1.
Marhavy P, Montesinos JC, Abuzeineh A, Van Damme D, Vermeer JE, Duclercq J, et al. Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation. GENES & DEVELOPMENT. 2016;30(4):471–83.
MLA
Marhavy, Peter, Juan Carlos Montesinos, Anas Abuzeineh, et al. “Targeted Cell Elimination Reveals an Auxin-guided Biphasic Mode of Lateral Root Initiation.” GENES & DEVELOPMENT 30.4 (2016): 471–483. Print.
@article{7169856,
  abstract     = {To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with a preserved proliferation capacity. The root pericycle represents a unique tissue with conditional meristematic activity, and its tight control determines initiation of lateral organs. Here we show that the meristematic activity of the pericycle is constrained by the interaction with the adjacent endodermis. Release of these restraints by elimination of endodermal cells by single-cell ablation triggers the pericycle to re-enter the cell cycle. We found that endodermis removal substitutes for the phytohormone auxin-dependent initiation of the pericycle meristematic activity. However, auxin is indispensable to steer the cell division plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally distinct role for auxin during lateral root initiation. In the endodermis, auxin releases constraints arising from cell-to-cell interactions that compromise the pericycle meristematic activity, whereas, in the pericycle, auxin defines the orientation of the cell division plane to initiate lateral roots.},
  author       = {Marhavy, Peter and Montesinos, Juan Carlos and Abuzeineh, Anas and Van Damme, Dani{\"e}l and Vermeer, Joop EM and Duclercq, J{\'e}r{\^o}me and Rakusova, Hana and Novakova, Petra and Friml, Jiri and Geldner, Niko and Benkova, Eva},
  issn         = {0890-9369},
  journal      = {GENES \& DEVELOPMENT},
  language     = {eng},
  number       = {4},
  pages        = {471--483},
  title        = {Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation},
  url          = {http://dx.doi.org/10.1101/gad.276964.115},
  volume       = {30},
  year         = {2016},
}

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