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Ascending aortic aneurysm in Angiotensin II–infused mice: formation, progression, and the role of focal dissections

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Abstract
Objective To understand the anatomy and physiology of ascending aortic aneurysms in angiotensin II-infused ApoE(-/-) mice. Approach and Results We combined an extensive in vivo imaging protocol (high-frequency ultrasound and contrast-enhanced microcomputed tomography at baseline and after 3, 10, 18, and 28 days of angiotensin II infusion) with synchrotron-based ultrahigh resolution ex vivo imaging (phase contrast X-ray tomographic microscopy) in n=47 angiotensin II-infused mice and 6 controls. Aortic regurgitation increased significantly over time, as did the luminal volume of the ascending aorta. In the samples that were scanned ex vivo, we observed one or several focal dissections, with the largest located in the outer convex aspect of the ascending aorta. The volume of the dissections moderately correlated to the volume of the aneurysm as measured in vivo (r(2)=0.46). After 3 days of angiotensin II infusion, we found an interlaminar hematoma in 7/12 animals, which could be linked to an intimal tear. There was also a significant increase in single laminar ruptures, which may have facilitated a progressive enlargement of the focal dissections over time. At later time points, the hematoma was resorbed and the medial and adventitial thickness increased. Fatal transmural dissection occurred in 8/47 mice at an early stage of the disease, before adventita remodeling. Conclusions We visualized and quantified the dissections that lead to ascending aortic aneurysms in angiotensin II-infused mice and provided unique insight into the temporal evolution of these lesions.
Keywords
DILATATION, VALVE DISEASE, CONVEXITY, PATTERNS, MEDIA, CELLS, MODEL, aortic aneurysm, aortic dissection, ascending aorta, cardiovascular imaging, mouse models of human disease

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Chicago
Trachet, Bram, Alessandra Piersigilli, Rodrigo Fraga-Silva, Lydia Aslanidou, Jessica Sordet-Dessimoz, Alberto Astolfo, Marco Stampanoni, Patrick Segers, and Nikolaos Stergiopulos. 2016. “Ascending Aortic Aneurysm in Angiotensin II–infused Mice: Formation, Progression, and the Role of Focal Dissections.” Arteriosclerosis Thrombosis and Vascular Biology 36 (4): 673–681.
APA
Trachet, B., Piersigilli, A., Fraga-Silva, R., Aslanidou, L., Sordet-Dessimoz, J., Astolfo, A., Stampanoni, M., et al. (2016). Ascending aortic aneurysm in Angiotensin II–infused mice: formation, progression, and the role of focal dissections. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 36(4), 673–681.
Vancouver
1.
Trachet B, Piersigilli A, Fraga-Silva R, Aslanidou L, Sordet-Dessimoz J, Astolfo A, et al. Ascending aortic aneurysm in Angiotensin II–infused mice: formation, progression, and the role of focal dissections. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY. 2016;36(4):673–81.
MLA
Trachet, Bram, Alessandra Piersigilli, Rodrigo Fraga-Silva, et al. “Ascending Aortic Aneurysm in Angiotensin II–infused Mice: Formation, Progression, and the Role of Focal Dissections.” ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 36.4 (2016): 673–681. Print.
@article{7161196,
  abstract     = {Objective To understand the anatomy and physiology of ascending aortic aneurysms in angiotensin II-infused ApoE(-/-) mice. 

Approach and Results We combined an extensive in vivo imaging protocol (high-frequency ultrasound and contrast-enhanced microcomputed tomography at baseline and after 3, 10, 18, and 28 days of angiotensin II infusion) with synchrotron-based ultrahigh resolution ex vivo imaging (phase contrast X-ray tomographic microscopy) in n=47 angiotensin II-infused mice and 6 controls. Aortic regurgitation increased significantly over time, as did the luminal volume of the ascending aorta. In the samples that were scanned ex vivo, we observed one or several focal dissections, with the largest located in the outer convex aspect of the ascending aorta. The volume of the dissections moderately correlated to the volume of the aneurysm as measured in vivo (r(2)=0.46). After 3 days of angiotensin II infusion, we found an interlaminar hematoma in 7/12 animals, which could be linked to an intimal tear. There was also a significant increase in single laminar ruptures, which may have facilitated a progressive enlargement of the focal dissections over time. At later time points, the hematoma was resorbed and the medial and adventitial thickness increased. Fatal transmural dissection occurred in 8/47 mice at an early stage of the disease, before adventita remodeling. 

Conclusions We visualized and quantified the dissections that lead to ascending aortic aneurysms in angiotensin II-infused mice and provided unique insight into the temporal evolution of these lesions.},
  author       = {Trachet, Bram and Piersigilli, Alessandra and Fraga-Silva, Rodrigo and Aslanidou, Lydia and Sordet-Dessimoz, Jessica and Astolfo, Alberto and Stampanoni, Marco and Segers, Patrick and Stergiopulos, Nikolaos},
  issn         = {1079-5642},
  journal      = {ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY},
  keyword      = {DILATATION,VALVE DISEASE,CONVEXITY,PATTERNS,MEDIA,CELLS,MODEL,aortic aneurysm,aortic dissection,ascending aorta,cardiovascular imaging,mouse models of human disease},
  language     = {eng},
  number       = {4},
  pages        = {673--681},
  title        = {Ascending aortic aneurysm in Angiotensin II--infused mice: formation, progression, and the role of focal dissections},
  url          = {http://dx.doi.org/10.1161/ATVBAHA.116.307211},
  volume       = {36},
  year         = {2016},
}

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