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Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction

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Abstract
In insects, the accessory gland proteins (Acps) secreted by male accessory glands (MAGs) account for the majority of seminal fluids proteins. Mixed with sperm, they are transferred to the female at mating and so impact reproduction. In this project, we identified 2,927 proteins in the MAG secretions of the oriental fruit fly Bactrocera dorsalis, an important agricultural pest worldwide, using LC-MS analysis, and all sequences containing open reading frames were analyzed using signalP. In total, 90Acps were identified. About one third (26) of these 90 Acps had a specific functional description, while the other two thirds (64) had no functional description including dozens of new classes of proteins. Hence, several of these novel Acps were abundant in the MAG secretions, and we confirmed their MAG-specific expression by qPCR. Finally and interestingly, one of these novel proteins was functionally predicted as juvenile hormone-binding protein, suggesting the impact of Acps with reproductive events in the female. Our results will aid in the development of an experimental method to identify Acps in insects, and in turn this information with new Acps in B. dorsalis will pave the way of further exploration their function in reproduction and potential development as new insecticide targets.
Keywords
GENOME-WIDE ANALYSIS, GENE-EXPRESSION, HONEYBEE APIS-MELLIFERA, TRIBOLIUM-CASTANEUM, ANOPHELES-GAMBIAE, HELICONIUS BUTTERFLIES, DENGUE VECTOR MOSQUITO, SEMINAL FLUID PROTEINS, DROSOPHILA-MELANOGASTER, CRICKET TELEOGRYLLUS-OCEANICUS

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MLA
Wei, Dong et al. “Proteome Analysis of Male Accessory Gland Secretions in Oriental Fruit Flies Reveals Juvenile Hormone-binding Protein, Suggesting Impact on Female Reproduction.” SCIENTIFIC REPORTS 5 (2015): n. pag. Print.
APA
Wei, D., Li, H.-M., Tian, C.-B., Smagghe, G., Jia, F.-X., Jiang, H.-B., Dou, W., et al. (2015). Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction. SCIENTIFIC REPORTS, 5.
Chicago author-date
Wei, Dong, Hui-Min Li, Chuan-Bei Tian, Guy Smagghe, Fu-Xian Jia, Hong-Bo Jiang, Wei Dou, and Jin-Jun Wang. 2015. “Proteome Analysis of Male Accessory Gland Secretions in Oriental Fruit Flies Reveals Juvenile Hormone-binding Protein, Suggesting Impact on Female Reproduction.” Scientific Reports 5.
Chicago author-date (all authors)
Wei, Dong, Hui-Min Li, Chuan-Bei Tian, Guy Smagghe, Fu-Xian Jia, Hong-Bo Jiang, Wei Dou, and Jin-Jun Wang. 2015. “Proteome Analysis of Male Accessory Gland Secretions in Oriental Fruit Flies Reveals Juvenile Hormone-binding Protein, Suggesting Impact on Female Reproduction.” Scientific Reports 5.
Vancouver
1.
Wei D, Li H-M, Tian C-B, Smagghe G, Jia F-X, Jiang H-B, et al. Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction. SCIENTIFIC REPORTS. 2015;5.
IEEE
[1]
D. Wei et al., “Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction,” SCIENTIFIC REPORTS, vol. 5, 2015.
@article{7150468,
  abstract     = {In insects, the accessory gland proteins (Acps) secreted by male accessory glands (MAGs) account for the majority of seminal fluids proteins. Mixed with sperm, they are transferred to the female at mating and so impact reproduction. In this project, we identified 2,927 proteins in the MAG secretions of the oriental fruit fly Bactrocera dorsalis, an important agricultural pest worldwide, using LC-MS analysis, and all sequences containing open reading frames were analyzed using signalP. In total, 90Acps were identified. About one third (26) of these 90 Acps had a specific functional description, while the other two thirds (64) had no functional description including dozens of new classes of proteins. Hence, several of these novel Acps were abundant in the MAG secretions, and we confirmed their MAG-specific expression by qPCR. Finally and interestingly, one of these novel proteins was functionally predicted as juvenile hormone-binding protein, suggesting the impact of Acps with reproductive events in the female. Our results will aid in the development of an experimental method to identify Acps in insects, and in turn this information with new Acps in B. dorsalis will pave the way of further exploration their function in reproduction and potential development as new insecticide targets.},
  articleno    = {16845},
  author       = {Wei, Dong and Li, Hui-Min and Tian, Chuan-Bei and Smagghe, Guy and Jia, Fu-Xian and Jiang, Hong-Bo and Dou, Wei and Wang, Jin-Jun},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {GENOME-WIDE ANALYSIS,GENE-EXPRESSION,HONEYBEE APIS-MELLIFERA,TRIBOLIUM-CASTANEUM,ANOPHELES-GAMBIAE,HELICONIUS BUTTERFLIES,DENGUE VECTOR MOSQUITO,SEMINAL FLUID PROTEINS,DROSOPHILA-MELANOGASTER,CRICKET TELEOGRYLLUS-OCEANICUS},
  language     = {eng},
  pages        = {12},
  title        = {Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction},
  url          = {http://dx.doi.org/10.1038/srep16845},
  volume       = {5},
  year         = {2015},
}

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