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Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions

Joke De Gelder, Peter Vandenabeele UGent, Patrick De Boever, Max Mergeay, Luc Moens UGent and Paul De Vos UGent (2009) MICROGRAVITY SCIENCE AND TECHNOLOGY. 21(3). p.217-223
abstract
In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-beta-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PHB, Metabolomics, BACTERIAL GENE-EXPRESSION, Cupriavidus metallidurans, Bacteria, Raman spectroscopy, Microgravity, LIFE-SUPPORT, IDENTIFICATION, MELISSA, METABOLISM, BIOREACTOR, SPACE-FLIGHT, SIMULATED MICROGRAVITY, ARTIFICIAL ECOSYSTEM, MODELED MICROGRAVITY
journal title
MICROGRAVITY SCIENCE AND TECHNOLOGY
Microgravity Sci. Technol.
volume
21
issue
3
pages
217 - 223
Web of Science type
Article
Web of Science id
000266830600001
JCR category
ENGINEERING, AEROSPACE
JCR impact factor
0.632 (2009)
JCR rank
10/27 (2009)
JCR quartile
2 (2009)
ISSN
0938-0108
DOI
10.1007/s12217-008-9037-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
714729
handle
http://hdl.handle.net/1854/LU-714729
date created
2009-07-07 11:30:40
date last changed
2012-11-09 14:12:39
@article{714729,
  abstract     = {In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-beta-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.},
  author       = {De Gelder, Joke and Vandenabeele, Peter and De Boever, Patrick and Mergeay, Max and Moens, Luc and De Vos, Paul},
  issn         = {0938-0108},
  journal      = {MICROGRAVITY SCIENCE AND TECHNOLOGY},
  keyword      = {PHB,Metabolomics,BACTERIAL GENE-EXPRESSION,Cupriavidus metallidurans,Bacteria,Raman spectroscopy,Microgravity,LIFE-SUPPORT,IDENTIFICATION,MELISSA,METABOLISM,BIOREACTOR,SPACE-FLIGHT,SIMULATED MICROGRAVITY,ARTIFICIAL ECOSYSTEM,MODELED MICROGRAVITY},
  language     = {eng},
  number       = {3},
  pages        = {217--223},
  title        = {Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions},
  url          = {http://dx.doi.org/10.1007/s12217-008-9037-0},
  volume       = {21},
  year         = {2009},
}

Chicago
De Gelder, Joke, Peter Vandenabeele, Patrick De Boever, Max Mergeay, Luc Moens, and Paul De Vos. 2009. “Raman Spectroscopic Analysis of Cupriavidus Metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions.” Microgravity Science and Technology 21 (3): 217–223.
APA
De Gelder, J., Vandenabeele, P., De Boever, P., Mergeay, M., Moens, L., & De Vos, P. (2009). Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions. MICROGRAVITY SCIENCE AND TECHNOLOGY, 21(3), 217–223.
Vancouver
1.
De Gelder J, Vandenabeele P, De Boever P, Mergeay M, Moens L, De Vos P. Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions. MICROGRAVITY SCIENCE AND TECHNOLOGY. 2009;21(3):217–23.
MLA
De Gelder, Joke, Peter Vandenabeele, Patrick De Boever, et al. “Raman Spectroscopic Analysis of Cupriavidus Metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions.” MICROGRAVITY SCIENCE AND TECHNOLOGY 21.3 (2009): 217–223. Print.