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Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions

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Abstract
In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-beta-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.
Keywords
Metabolomics, BACTERIAL GENE-EXPRESSION, PHB, Cupriavidus metallidurans, Bacteria, Raman spectroscopy, Microgravity, LIFE-SUPPORT, IDENTIFICATION, MELISSA, METABOLISM, BIOREACTOR, SPACE-FLIGHT, SIMULATED MICROGRAVITY, ARTIFICIAL ECOSYSTEM, MODELED MICROGRAVITY

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MLA
De Gelder, Joke, et al. “Raman Spectroscopic Analysis of Cupriavidus Metallidurans LMG 1195 (CH34) Cultured in Low-Shear Microgravity Conditions.” MICROGRAVITY SCIENCE AND TECHNOLOGY, vol. 21, no. 3, 2009, pp. 217–23.
APA
De Gelder, J., Vandenabeele, P., De Boever, P., Mergeay, M., Moens, L., & De Vos, P. (2009). Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions. MICROGRAVITY SCIENCE AND TECHNOLOGY, 21(3), 217–223.
Chicago author-date
De Gelder, Joke, Peter Vandenabeele, Patrick De Boever, Max Mergeay, Luc Moens, and Paul De Vos. 2009. “Raman Spectroscopic Analysis of Cupriavidus Metallidurans LMG 1195 (CH34) Cultured in Low-Shear Microgravity Conditions.” MICROGRAVITY SCIENCE AND TECHNOLOGY 21 (3): 217–23.
Chicago author-date (all authors)
De Gelder, Joke, Peter Vandenabeele, Patrick De Boever, Max Mergeay, Luc Moens, and Paul De Vos. 2009. “Raman Spectroscopic Analysis of Cupriavidus Metallidurans LMG 1195 (CH34) Cultured in Low-Shear Microgravity Conditions.” MICROGRAVITY SCIENCE AND TECHNOLOGY 21 (3): 217–223.
Vancouver
1.
De Gelder J, Vandenabeele P, De Boever P, Mergeay M, Moens L, De Vos P. Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions. MICROGRAVITY SCIENCE AND TECHNOLOGY. 2009;21(3):217–23.
IEEE
[1]
J. De Gelder, P. Vandenabeele, P. De Boever, M. Mergeay, L. Moens, and P. De Vos, “Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions,” MICROGRAVITY SCIENCE AND TECHNOLOGY, vol. 21, no. 3, pp. 217–223, 2009.
@article{714729,
  abstract     = {{In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a rotating wall vessel to simulate microgravity (SMG) and in a control setup. The differences in Raman spectra recorded from both setups after 24 h of culturing were small. The most prominent features in a difference spectrum, calculated between the mean spectra from the microgravity and the control setup separately, could be assigned to the presence of poly-beta-hydroxybutyrate (PHB). SMG seems to yield a higher PHB production after 24 h of culturing. Additional processing of the spectra suggested that SMG induced also other changes in the carbon-metabolism. After 48 h, similar results were found for the carbon metabolism, while PHB concentrations were reduced in SMG compared to the control. However, these differences could also be caused by interfering effects that may occur in the bioreactors after a prolonged incubation time.}},
  author       = {{De Gelder, Joke and Vandenabeele, Peter and De Boever, Patrick and Mergeay, Max and Moens, Luc and De Vos, Paul}},
  issn         = {{0938-0108}},
  journal      = {{MICROGRAVITY SCIENCE AND TECHNOLOGY}},
  keywords     = {{Metabolomics,BACTERIAL GENE-EXPRESSION,PHB,Cupriavidus metallidurans,Bacteria,Raman spectroscopy,Microgravity,LIFE-SUPPORT,IDENTIFICATION,MELISSA,METABOLISM,BIOREACTOR,SPACE-FLIGHT,SIMULATED MICROGRAVITY,ARTIFICIAL ECOSYSTEM,MODELED MICROGRAVITY}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{217--223}},
  title        = {{Raman spectroscopic analysis of Cupriavidus metallidurans LMG 1195 (CH34) cultured in low-shear microgravity conditions}},
  url          = {{http://dx.doi.org/10.1007/s12217-008-9037-0}},
  volume       = {{21}},
  year         = {{2009}},
}

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