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Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza

Yu Qiu (UGent) , Karl De hert (UGent) and Kristien Van Reeth (UGent)
Author
Organization
Project
RT 09/6227
Project
FP7-GA 258084
Abstract
Pigs are natural hosts for the same influenza virus subtypes as humans and are a valuable model for cross-protection studies with influenza. In this study, we have used the pig model to examine the extent of virological protection between a) the 2009 pandemic H1N1 (pH1N1) virus and three different European H1 swine influenza virus (SIV) lineages, and b) these H1 viruses and a European H3N2 SIV. Pigs were inoculated intranasally with representative strains of each virus lineage with 6- and 17-week intervals between H1 inoculations and between H1 and H3 inoculations, respectively. Virus titers in nasal swabs and/or tissues of the respiratory tract were determined after each inoculation. There was substantial though differing cross-protection between pH1N1 and other H1 viruses, which was directly correlated with the relatedness in the viral hemagglutinin (HA) and neuraminidase (NA) proteins. Cross-protection against H3N2 was almost complete in pigs with immunity against H1N2, but was weak in H1N1/pH1N1-immune pigs. In conclusion, infection with a live, wild type influenza virus may offer substantial cross-lineage protection against viruses of the same HA and/or NA subtype. True heterosubtypic protection, in contrast, appears to be minimal in natural influenza virus hosts. We discuss our findings in the light of the zoonotic and pandemic risks of SIVs.
Keywords
A VIRUS, GENETIC REASSORTMENT, H3N2 VIRUS, NEURAMINIDASE, MICE, HUMANS, TRANSMISSION, ANTIBODIES, ORIGIN, RESPONSES

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Citation

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Chicago
Qiu, Yu, De hertKarl, and Kristien Van Reeth. 2015. “Cross-protection Against European Swine Influenza Viruses in the Context of Infection Immunity Against the 2009 Pandemic H1N1 Virus : Studies in the Pig Model of Influenza.” Veterinary Research 46.
APA
Qiu, Y., De hertKarl, & Van Reeth, K. (2015). Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza. VETERINARY RESEARCH, 46.
Vancouver
1.
Qiu Y, De hertKarl, Van Reeth K. Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza. VETERINARY RESEARCH. 2015;46.
MLA
Qiu, Yu, De hertKarl, and Kristien Van Reeth. “Cross-protection Against European Swine Influenza Viruses in the Context of Infection Immunity Against the 2009 Pandemic H1N1 Virus : Studies in the Pig Model of Influenza.” VETERINARY RESEARCH 46 (2015): n. pag. Print.
@article{7147264,
  abstract     = {Pigs are natural hosts for the same influenza virus subtypes as humans and are a valuable model for cross-protection studies with influenza. In this study, we have used the pig model to examine the extent of virological protection between a) the 2009 pandemic H1N1 (pH1N1) virus and three different European H1 swine influenza virus (SIV) lineages, and b) these H1 viruses and a European H3N2 SIV. Pigs were inoculated intranasally with representative strains of each virus lineage with 6- and 17-week intervals between H1 inoculations and between H1 and H3 inoculations, respectively. Virus titers in nasal swabs and/or tissues of the respiratory tract were determined after each inoculation. There was substantial though differing cross-protection between pH1N1 and other H1 viruses, which was directly correlated with the relatedness in the viral hemagglutinin (HA) and neuraminidase (NA) proteins. Cross-protection against H3N2 was almost complete in pigs with immunity against H1N2, but was weak in H1N1/pH1N1-immune pigs. In conclusion, infection with a live, wild type influenza virus may offer substantial cross-lineage protection against viruses of the same HA and/or NA subtype. True heterosubtypic protection, in contrast, appears to be minimal in natural influenza virus hosts. We discuss our findings in the light of the zoonotic and pandemic risks of SIVs.},
  articleno    = {105},
  author       = {Qiu, Yu and De hert, Karl and Van Reeth, Kristien},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {A VIRUS,GENETIC REASSORTMENT,H3N2 VIRUS,NEURAMINIDASE,MICE,HUMANS,TRANSMISSION,ANTIBODIES,ORIGIN,RESPONSES},
  language     = {eng},
  pages        = {10},
  title        = {Cross-protection against European swine influenza viruses in the context of infection immunity against the 2009 pandemic H1N1 virus : studies in the pig model of influenza},
  url          = {http://dx.doi.org/10.1186/s13567-015-0236-6},
  volume       = {46},
  year         = {2015},
}

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