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Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation

Wen-kun Huang, Hongli Ji (UGent) , Godelieve Gheysen (UGent) and Tina Kyndt (UGent)
(2016) MOLECULAR PLANT PATHOLOGY. 17(4). p.614-624
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Abstract
Thiamine (vitamin B1, VB1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion. In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated. Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide (H2O2) accumulation and lignin deposition in plant roots, and this correlated with enhanced transcription of OsPAL1 and OsC4H, two genes involved in the phenylpropanoid pathway. The number of nematodes in rice roots was slightly but significantly reduced, and the development of the nematodes was delayed, whereas no direct toxic effects of VB1 on nematode viability and infectivity were observed. The combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia-lyase (PAL), significantly hampered the VB1-priming capacity. These findings indicate that thiamine-induced priming in rice involves H2O2 and phenylpropanoid-mediated lignin production, which hampers nematode infection. Further cellular and molecular studies on the mechanism of thiamine-induced defence will be useful for the development of novel nematode control strategies.
Keywords
callose, hydrogen peroxide, induced defence, lignin, Meloidogyne graminicola, priming, thiamine, ACID-INDUCED RESISTANCE, LIGNIN BIOSYNTHESIS, DISEASE RESISTANCE, GENE-EXPRESSION, NADPH OXIDASE, DOWNY MILDEW, ARABIDOPSIS, DEFENSE, CALLOSE, ACCUMULATION

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Citation

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MLA
Huang, Wen-kun, et al. “Thiamine-Induced Priming against Root-Knot Nematode Infection in Rice Involves Lignification and Hydrogen Peroxide Generation.” MOLECULAR PLANT PATHOLOGY, vol. 17, no. 4, 2016, pp. 614–24, doi:10.1111/mpp.12316.
APA
Huang, W., Ji, H., Gheysen, G., & Kyndt, T. (2016). Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation. MOLECULAR PLANT PATHOLOGY, 17(4), 614–624. https://doi.org/10.1111/mpp.12316
Chicago author-date
Huang, Wen-kun, Hongli Ji, Godelieve Gheysen, and Tina Kyndt. 2016. “Thiamine-Induced Priming against Root-Knot Nematode Infection in Rice Involves Lignification and Hydrogen Peroxide Generation.” MOLECULAR PLANT PATHOLOGY 17 (4): 614–24. https://doi.org/10.1111/mpp.12316.
Chicago author-date (all authors)
Huang, Wen-kun, Hongli Ji, Godelieve Gheysen, and Tina Kyndt. 2016. “Thiamine-Induced Priming against Root-Knot Nematode Infection in Rice Involves Lignification and Hydrogen Peroxide Generation.” MOLECULAR PLANT PATHOLOGY 17 (4): 614–624. doi:10.1111/mpp.12316.
Vancouver
1.
Huang W, Ji H, Gheysen G, Kyndt T. Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation. MOLECULAR PLANT PATHOLOGY. 2016;17(4):614–24.
IEEE
[1]
W. Huang, H. Ji, G. Gheysen, and T. Kyndt, “Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation,” MOLECULAR PLANT PATHOLOGY, vol. 17, no. 4, pp. 614–624, 2016.
@article{7144921,
  abstract     = {{Thiamine (vitamin B1, VB1) can act as a plant defence trigger, or priming agent, leading to a rapid counterattack on pathogen invasion. In this study, the priming effect of thiamine on rice (Oryza sativa cv. Nipponbare) and its activity against root-knot nematode (Meloidogyne graminicola) infection were evaluated. Thiamine treatment and subsequent nematode inoculation activated hydrogen peroxide (H2O2) accumulation and lignin deposition in plant roots, and this correlated with enhanced transcription of OsPAL1 and OsC4H, two genes involved in the phenylpropanoid pathway. The number of nematodes in rice roots was slightly but significantly reduced, and the development of the nematodes was delayed, whereas no direct toxic effects of VB1 on nematode viability and infectivity were observed. The combined application of thiamine with l-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia-lyase (PAL), significantly hampered the VB1-priming capacity. These findings indicate that thiamine-induced priming in rice involves H2O2 and phenylpropanoid-mediated lignin production, which hampers nematode infection. Further cellular and molecular studies on the mechanism of thiamine-induced defence will be useful for the development of novel nematode control strategies.}},
  author       = {{Huang, Wen-kun and Ji, Hongli and Gheysen, Godelieve and Kyndt, Tina}},
  issn         = {{1464-6722}},
  journal      = {{MOLECULAR PLANT PATHOLOGY}},
  keywords     = {{callose,hydrogen peroxide,induced defence,lignin,Meloidogyne graminicola,priming,thiamine,ACID-INDUCED RESISTANCE,LIGNIN BIOSYNTHESIS,DISEASE RESISTANCE,GENE-EXPRESSION,NADPH OXIDASE,DOWNY MILDEW,ARABIDOPSIS,DEFENSE,CALLOSE,ACCUMULATION}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{614--624}},
  title        = {{Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation}},
  url          = {{http://doi.org/10.1111/mpp.12316}},
  volume       = {{17}},
  year         = {{2016}},
}
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