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Beta-catenin and plakoglobin expression during zebrafish tooth development and replacement

Barbara Verstraeten (UGent) , Jolanda van Hengel (UGent) and Ann Huysseune (UGent)
(2016) PLOS ONE. 11(3).
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Abstract
We analyzed the protein distribution of two cadherin-associated molecules, plakoglobin and β-catenin, during the different stages of tooth development and tooth replacement in zebrafish. Plakoglobin was detected at the plasma membrane already at the onset of tooth development in the epithelial cells of the tooth. This pattern remained unaltered during further tooth development. The mesenchymal cells only showed plakoglobin from cytodifferentiation onwards. Plakoglobin 1a morpholino-injected embryos showed normal tooth development with proper initiation and differentiation. Although plakoglobin is clearly present during normal odontogenesis, the loss of plakoglobin 1a does not influence tooth development. β-catenin was found at the cell borders of all cells of the successional lamina but also in the nuclei of surrounding mesenchymal cells. Only membranous, not nuclear, β-catenin, was found during morphogenesis stage. However, during cytodifferentiation stage, both nuclear and membrane-bound β-catenin was detected in the layers of the enamel organ as well as in the differentiating odontoblasts. Nuclear β-catenin is an indication of an activated Wnt pathway, therefore suggesting a possible role for Wnt signalling during zebrafish tooth development and replacement.
Keywords
DANIO-RERIO TELEOSTEI, CELL-CELL ADHESION, WNT SIGNALING PATHWAY, E-CADHERIN, ADHERENS JUNCTIONS, TISSUE MORPHOGENESIS, EMBRYONIC HEART, 1ST TOOTH, MOUSE, LOCALIZATION

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Chicago
Verstraeten, Barbara, Jolanda van Hengel, and Ann Huysseune. 2016. “Beta-catenin and Plakoglobin Expression During Zebrafish Tooth Development and Replacement.” Plos One 11 (3).
APA
Verstraeten, B., van Hengel, J., & Huysseune, A. (2016). Beta-catenin and plakoglobin expression during zebrafish tooth development and replacement. PLOS ONE, 11(3).
Vancouver
1.
Verstraeten B, van Hengel J, Huysseune A. Beta-catenin and plakoglobin expression during zebrafish tooth development and replacement. PLOS ONE. 2016;11(3).
MLA
Verstraeten, Barbara, Jolanda van Hengel, and Ann Huysseune. “Beta-catenin and Plakoglobin Expression During Zebrafish Tooth Development and Replacement.” PLOS ONE 11.3 (2016): n. pag. Print.
@article{7140393,
  abstract     = {We analyzed the protein distribution of two cadherin-associated molecules, plakoglobin and \ensuremath{\beta}-catenin, during the different stages of tooth development and tooth replacement in zebrafish. Plakoglobin was detected at the plasma membrane already at the onset of tooth development in the epithelial cells of the tooth. This pattern remained unaltered during further tooth development. The mesenchymal cells only showed plakoglobin from cytodifferentiation onwards. Plakoglobin 1a morpholino-injected embryos showed normal tooth development with proper initiation and differentiation. Although plakoglobin is clearly present during normal odontogenesis, the loss of plakoglobin 1a does not influence tooth development. \ensuremath{\beta}-catenin was found at the cell borders of all cells of the successional lamina but also in the nuclei of surrounding mesenchymal cells. Only membranous, not nuclear, \ensuremath{\beta}-catenin, was found during morphogenesis stage. However, during cytodifferentiation stage, both nuclear and membrane-bound \ensuremath{\beta}-catenin was detected in the layers of the enamel organ as well as in the differentiating odontoblasts. Nuclear \ensuremath{\beta}-catenin is an indication of an activated Wnt pathway, therefore suggesting a possible role for Wnt signalling during zebrafish tooth development and replacement.},
  articleno    = {e0148114},
  author       = {Verstraeten, Barbara and van Hengel, Jolanda and Huysseune, Ann},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {DANIO-RERIO TELEOSTEI,CELL-CELL ADHESION,WNT SIGNALING PATHWAY,E-CADHERIN,ADHERENS JUNCTIONS,TISSUE MORPHOGENESIS,EMBRYONIC HEART,1ST TOOTH,MOUSE,LOCALIZATION},
  language     = {eng},
  number       = {3},
  pages        = {14},
  title        = {Beta-catenin and plakoglobin expression during zebrafish tooth development and replacement},
  url          = {http://dx.doi.org/10.1371/journal.pone.0148114},
  volume       = {11},
  year         = {2016},
}

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