Advanced search
1 file | 939.50 KB

A livestock-associated, multidrug-resistant, methicillin-resistant Staphylococcus aureus clonal complex 97 lineage spreading in dairy cattle and pigs in Italy

Author
Organization
Abstract
Pandemic methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 97 (CC97) lineages originated from livestock-to-human host jumps. In recent years, CC97 has become one of the major MRSA lineages detected in Italian farmed animals. The aim of this study was to characterize and analyze differences in MRSA and methicillin-susceptible S. aureus (MSSA) mainly of swine and bovine origins. Forty-seven CC97 isolates, 35 MRSA isolates, and 6 MSSA isolates from different Italian pig and cattle holdings; 5 pig MRSA isolates from Germany; and 1 human MSSA isolate from Spain were characterized by macrorestriction pulsed-field gel electrophoresis (PFGE) analysis, multilocus sequence typing (MLST), spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and antimicrobial resistance pattern analysis. Virulence and resistance genes were investigated by PCR and microarray analysis. Most of the isolates were of SCCmec type V (SCCmec V), except for two German MRSA isolates (SCCmec III). Five main clusters were identified by PFGE, with the German isolates (clusters I and II) showing 60.5% similarity with the Italian isolates, most of which (68.1%) grouped into cluster V. All CC97 isolates were Panton-Valentine leukocidin (PVL) negative, and a few (n = 7) tested positive for sak or scn. All MRSA isolates were multidrug resistant (MDR), and the main features were erm(B)-or erm(C)-mediated (n = 18) macrolide-lincosamide-streptogramin B resistance, vga(A)-mediated (n = 37) pleuromutilin resistance, fluoroquinolone resistance (n = 33), tet(K) in 32/37 tet(M)-positive isolates, and blaZ in almost all MRSA isolates. Few host-associated differences were detected among CC97 MRSA isolates: their extensive MDR nature in both pigs and dairy cattle may be a consequence of a spillback from pigs of a MRSA lineage that originated in cattle as MSSA and needs further investigation. Measures should be implemented at the farm level to prevent spillover to humans in intensive farming areas.
Keywords
PROTEIN, ANIMALS, MULTIPLEX PCR ASSAY, FIELD GEL-ELECTROPHORESIS, STRAINS, GENES, MECA, BAP

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 939.50 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Feltrin, Fabiola, Patricia Alba, Britta Kraushaar, Angela Ianzano, María Angeles Argudín, Paola Di Matteo, María Concepcíon Porrero, et al. 2016. “A Livestock-associated, Multidrug-resistant, Methicillin-resistant Staphylococcus Aureus Clonal Complex 97 Lineage Spreading in Dairy Cattle and Pigs in Italy.” Applied and Environmental Microbiology 82 (3): 816–821.
APA
Feltrin, F., Alba, P., Kraushaar, B., Ianzano, A., Argudín, M. A., Di Matteo, P., Porrero, M. C., et al. (2016). A livestock-associated, multidrug-resistant, methicillin-resistant Staphylococcus aureus clonal complex 97 lineage spreading in dairy cattle and pigs in Italy. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(3), 816–821.
Vancouver
1.
Feltrin F, Alba P, Kraushaar B, Ianzano A, Argudín MA, Di Matteo P, et al. A livestock-associated, multidrug-resistant, methicillin-resistant Staphylococcus aureus clonal complex 97 lineage spreading in dairy cattle and pigs in Italy. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2016;82(3):816–21.
MLA
Feltrin, Fabiola, Patricia Alba, Britta Kraushaar, et al. “A Livestock-associated, Multidrug-resistant, Methicillin-resistant Staphylococcus Aureus Clonal Complex 97 Lineage Spreading in Dairy Cattle and Pigs in Italy.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.3 (2016): 816–821. Print.
@article{7139244,
  abstract     = {Pandemic methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 97 (CC97) lineages originated from livestock-to-human host jumps. In recent years, CC97 has become one of the major MRSA lineages detected in Italian farmed animals. The aim of this study was to characterize and analyze differences in MRSA and methicillin-susceptible S. aureus (MSSA) mainly of swine and bovine origins. Forty-seven CC97 isolates, 35 MRSA isolates, and 6 MSSA isolates from different Italian pig and cattle holdings; 5 pig MRSA isolates from Germany; and 1 human MSSA isolate from Spain were characterized by macrorestriction pulsed-field gel electrophoresis (PFGE) analysis, multilocus sequence typing (MLST), spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and antimicrobial resistance pattern analysis. Virulence and resistance genes were investigated by PCR and microarray analysis. Most of the isolates were of SCCmec type V (SCCmec V), except for two German MRSA isolates (SCCmec III). Five main clusters were identified by PFGE, with the German isolates (clusters I and II) showing 60.5\% similarity with the Italian isolates, most of which (68.1\%) grouped into cluster V. All CC97 isolates were Panton-Valentine leukocidin (PVL) negative, and a few (n = 7) tested positive for sak or scn. All MRSA isolates were multidrug resistant (MDR), and the main features were erm(B)-or erm(C)-mediated (n = 18) macrolide-lincosamide-streptogramin B resistance, vga(A)-mediated (n = 37) pleuromutilin resistance, fluoroquinolone resistance (n = 33), tet(K) in 32/37 tet(M)-positive isolates, and blaZ in almost all MRSA isolates. Few host-associated differences were detected among CC97 MRSA isolates: their extensive MDR nature in both pigs and dairy cattle may be a consequence of a spillback from pigs of a MRSA lineage that originated in cattle as MSSA and needs further investigation. Measures should be implemented at the farm level to prevent spillover to humans in intensive farming areas.},
  author       = {Feltrin, Fabiola and Alba, Patricia and Kraushaar, Britta and Ianzano, Angela and Argud{\'i}n, Mar{\'i}a Angeles and Di Matteo, Paola and Porrero, Mar{\'i}a Concepc{\'i}on and Aarestrup, Frank M and Butaye, Patrick and Franco, Alessia and Battisti, Antonio},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PROTEIN,ANIMALS,MULTIPLEX PCR ASSAY,FIELD GEL-ELECTROPHORESIS,STRAINS,GENES,MECA,BAP},
  language     = {eng},
  number       = {3},
  pages        = {816--821},
  title        = {A livestock-associated, multidrug-resistant, methicillin-resistant Staphylococcus aureus clonal complex 97 lineage spreading in dairy cattle and pigs in Italy},
  url          = {http://dx.doi.org/10.1128/AEM.02854-15},
  volume       = {82},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: