Advanced search
1 file | 1.56 MB

Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies

Author
Organization
Abstract
Helicobacter (H.) suis causes gastric pathologies in both pigs and humans. Very little is known on the metabolism of this bacterium and its impact on the host. In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) in the medium during H. suis culture. Besides Gln, H. suis can also convert glutathione (GSH) to glutamate, and both reactions are catalyzed by the H. suis gamma-glutamyltranspeptidase (GGT). Both for H. pylori and H. suis, it has been hypothesized that the degradation of Gln and GSH may lead to a deficiency for the host, possibly initiating or promoting several pathologies. Therefore the in vivo effect of oral supplementation with Gln and GSH was assessed. Oral supplementation with Gln was shown to temper H. suis induced gastritis and epithelial (hyper) proliferation in Mongolian gerbils. Astonishingly, supplementation of the feed with GSH, another GGT substrate, resulted in inflammation and epithelial proliferation levels returning to baseline levels of uninfected controls. This indicates that Gln and GSH supplementation may help reducing tissue damage caused by Helicobacter infection in both humans and pigs, highlighting their potential as a supportive therapy during and after Helicobacter eradication therapy.
Keywords
MALT LYMPHOMA, INDUCED APOPTOSIS, EPITHELIAL-CELLS, MUCOSA, SUIS INFECTION, PYLORI INFECTION, GAMMA-GLUTAMYL-TRANSPEPTIDASE, INTESTINAL FUNCTION, HEILMANNII, SENSU-STRICTO

Downloads

  • de bruyne.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.56 MB

Citation

Please use this url to cite or link to this publication:

Chicago
De Bruyne, Ellen, Richard Ducatelle, Dennis Foss, Margaret Sanchez, Myrthe Joosten, Guangzhi Zhang, Annemieke Smet, Frank Pasmans, Freddy Haesebrouck, and Bram Flahou. 2016. “Oral Glutathione Supplementation Drastically Reduces Helicobacter-induced Gastric Pathologies.” Scientific Reports 6.
APA
De Bruyne, Ellen, Ducatelle, R., Foss, D., Sanchez, M., Joosten, M., Zhang, G., Smet, A., et al. (2016). Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies. SCIENTIFIC REPORTS, 6.
Vancouver
1.
De Bruyne E, Ducatelle R, Foss D, Sanchez M, Joosten M, Zhang G, et al. Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies. SCIENTIFIC REPORTS. 2016;6.
MLA
De Bruyne, Ellen et al. “Oral Glutathione Supplementation Drastically Reduces Helicobacter-induced Gastric Pathologies.” SCIENTIFIC REPORTS 6 (2016): n. pag. Print.
@article{7137973,
  abstract     = {Helicobacter (H.) suis causes gastric pathologies in both pigs and humans. Very little is known on the metabolism of this bacterium and its impact on the host. In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) in the medium during H. suis culture. Besides Gln, H. suis can also convert glutathione (GSH) to glutamate, and both reactions are catalyzed by the H. suis gamma-glutamyltranspeptidase (GGT). Both for H. pylori and H. suis, it has been hypothesized that the degradation of Gln and GSH may lead to a deficiency for the host, possibly initiating or promoting several pathologies. Therefore the in vivo effect of oral supplementation with Gln and GSH was assessed. Oral supplementation with Gln was shown to temper H. suis induced gastritis and epithelial (hyper) proliferation in Mongolian gerbils. Astonishingly, supplementation of the feed with GSH, another GGT substrate, resulted in inflammation and epithelial proliferation levels returning to baseline levels of uninfected controls. This indicates that Gln and GSH supplementation may help reducing tissue damage caused by Helicobacter infection in both humans and pigs, highlighting their potential as a supportive therapy during and after Helicobacter eradication therapy.},
  articleno    = {20169},
  author       = {De Bruyne, Ellen and Ducatelle, Richard and Foss, Dennis and Sanchez, Margaret and Joosten, Myrthe and Zhang, Guangzhi and Smet, Annemieke and Pasmans, Frank and Haesebrouck, Freddy and Flahou, Bram},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {MALT LYMPHOMA,INDUCED APOPTOSIS,EPITHELIAL-CELLS,MUCOSA,SUIS INFECTION,PYLORI INFECTION,GAMMA-GLUTAMYL-TRANSPEPTIDASE,INTESTINAL FUNCTION,HEILMANNII,SENSU-STRICTO},
  language     = {eng},
  pages        = {13},
  title        = {Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies},
  url          = {http://dx.doi.org/10.1038/srep20169},
  volume       = {6},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: