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Determination of five folate monoglutamates in rodent diets

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Abstract
A method for the quantitative determination of folates in rodent diets is very important for correct interpretation of folate intake during feeding trials, given the possible discrepancy between the actual folate concentration in the diet and that mentioned on the product sheet. Liquid chromatography tandem-mass spectrometry is the method of choice to differentiate and quantify the individual folate species present. This discrepancy may be accounted for by, e.g., inaccurate folic acid supplementation and/or the presence of endogenous reduced and substituted folates. We developed a method, validated based on FDA guidelines, that allows the measurement of added and endogenous folates by quantitative determination of 5 folate monoglutamates with linear ranges from 8 mu g to 2 mg/kg feed. This information, combined with feed intake data, allows insight into the actual folate intake in animal feeding studies. The relevance of this method was illustrated by the analysis of several feed samples of varying composition, by the investigation of the effect of casein incorporation, and by evaluating the variability of the folate content between pellets and production batches.
Keywords
AMYLASE, ASSAY, CONJUGASE, STABILITY, HYDROLASE, DNA METHYLATION, FOODS, FOLIC-ACID, TANDEM MASS-SPECTROMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, mass spectrometry, liquid chromatography, rodent diet, folate

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Chicago
Kiekens, Filip, Jeroen Van Daele, Dieter Blancquaert, Dominique Van Der Straeten, Willy Lambert, and Christophe Stove. 2015. “Determination of Five Folate Monoglutamates in Rodent Diets.” Journal of Agricultural and Food Chemistry 63 (45): 10089–10095.
APA
Kiekens, F., Van Daele, J., Blancquaert, D., Van Der Straeten, D., Lambert, W., & Stove, C. (2015). Determination of five folate monoglutamates in rodent diets. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63(45), 10089–10095.
Vancouver
1.
Kiekens F, Van Daele J, Blancquaert D, Van Der Straeten D, Lambert W, Stove C. Determination of five folate monoglutamates in rodent diets. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2015;63(45):10089–95.
MLA
Kiekens, Filip, Jeroen Van Daele, Dieter Blancquaert, et al. “Determination of Five Folate Monoglutamates in Rodent Diets.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.45 (2015): 10089–10095. Print.
@article{7137319,
  abstract     = {A method for the quantitative determination of folates in rodent diets is very important for correct interpretation of folate intake during feeding trials, given the possible discrepancy between the actual folate concentration in the diet and that mentioned on the product sheet. Liquid chromatography tandem-mass spectrometry is the method of choice to differentiate and quantify the individual folate species present. This discrepancy may be accounted for by, e.g., inaccurate folic acid supplementation and/or the presence of endogenous reduced and substituted folates. We developed a method, validated based on FDA guidelines, that allows the measurement of added and endogenous folates by quantitative determination of 5 folate monoglutamates with linear ranges from 8 mu g to 2 mg/kg feed. This information, combined with feed intake data, allows insight into the actual folate intake in animal feeding studies. The relevance of this method was illustrated by the analysis of several feed samples of varying composition, by the investigation of the effect of casein incorporation, and by evaluating the variability of the folate content between pellets and production batches.},
  author       = {Kiekens, Filip and Van Daele, Jeroen and Blancquaert, Dieter and Van Der Straeten, Dominique and Lambert, Willy and Stove, Christophe},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  language     = {eng},
  number       = {45},
  pages        = {10089--10095},
  title        = {Determination of five folate monoglutamates in rodent diets},
  url          = {http://dx.doi.org/10.1021/acs.jafc.5b04075},
  volume       = {63},
  year         = {2015},
}

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