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The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale

Sarah De Laethauwer UGent, Dirk Reheul UGent, Jan De Riek and Geert Haesaert UGent (2009) EUPHYTICA. 168(3). p.379-384
abstract
In spite of the availability of laboratory and field tests there is still a major problem to select pre-harvest sprouting (PHS) tolerant triticale varieties in a reliable, field-independent way. One approach to minimize the influence of environmental conditions and physio-morphological traits on PHS detection is using molecular genetic tools. The 'viviparous' Vp1 gene has been repeatedly described to play an important role in dormancy in wheat. A quantitative RT-PCR assay based on the expression of the Vp1 gene has been developed. Specific primers were designed for detecting Vp1 in both wheat and triticale. The expression levels of Vp1 were normalized using reference genes and relatively quantified with the comparative C-t-method. However, the first results indicate that the achieved Vp1 expression levels at 50 days post anthesis are not useful to select for PHS tolerance, both in wheat and triticale. This negative outcome so far is possibly due to the existence of several splicing events or to the late assaying moment in the kernel development, when Vp1 expression is found to be low.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
Triticale, Wheat, Vp1 gene, HOMOLOG, MAIZE, TRANSCRIPTS, EXPRESSION, CULTIVARS, VIVIPAROUS-1, DORMANCY, WHEAT, EMBRYO DEVELOPMENT, THUMB DWARFING GENE, Pre-harvest sprouting, Real time RT-PCR
journal title
EUPHYTICA
Euphytica
volume
168
issue
3
pages
379 - 384
conference name
11th International Symposium on Pre-Harvest Sprouting in Cereals
conference location
Mendoza, Argentina
conference start
2007-11-05
conference end
2007-11-08
Web of Science type
Proceedings Paper
Web of Science id
000267592000009
ISSN
1573-5060
DOI
10.1007/s10681-009-9930-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
713652
handle
http://hdl.handle.net/1854/LU-713652
date created
2009-07-03 13:25:29
date last changed
2009-11-13 12:24:23
@article{713652,
  abstract     = {In spite of the availability of laboratory and field tests there is still a major problem to select pre-harvest sprouting (PHS) tolerant triticale varieties in a reliable, field-independent way. One approach to minimize the influence of environmental conditions and physio-morphological traits on PHS detection is using molecular genetic tools. The 'viviparous' Vp1 gene has been repeatedly described to play an important role in dormancy in wheat. A quantitative RT-PCR assay based on the expression of the Vp1 gene has been developed. Specific primers were designed for detecting Vp1 in both wheat and triticale. The expression levels of Vp1 were normalized using reference genes and relatively quantified with the comparative C-t-method. However, the first results indicate that the achieved Vp1 expression levels at 50 days post anthesis are not useful to select for PHS tolerance, both in wheat and triticale. This negative outcome so far is possibly due to the existence of several splicing events or to the late assaying moment in the kernel development, when Vp1 expression is found to be low.},
  author       = {De Laethauwer, Sarah and Reheul, Dirk and De Riek, Jan and Haesaert, Geert},
  issn         = {1573-5060},
  journal      = {EUPHYTICA},
  keyword      = {Triticale,Wheat,Vp1 gene,HOMOLOG,MAIZE,TRANSCRIPTS,EXPRESSION,CULTIVARS,VIVIPAROUS-1,DORMANCY,WHEAT,EMBRYO DEVELOPMENT,THUMB DWARFING GENE,Pre-harvest sprouting,Real time RT-PCR},
  language     = {eng},
  location     = {Mendoza, Argentina},
  number       = {3},
  pages        = {379--384},
  title        = {The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale},
  url          = {http://dx.doi.org/10.1007/s10681-009-9930-7},
  volume       = {168},
  year         = {2009},
}

Chicago
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, and Geert Haesaert. 2009. “The Use of Vp1 in Real Time RT-PCR to Select for Pre-harvest Sprouting Tolerance in Triticale.” Euphytica 168 (3): 379–384.
APA
De Laethauwer, S., Reheul, D., De Riek, J., & Haesaert, G. (2009). The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale. EUPHYTICA, 168(3), 379–384. Presented at the 11th International Symposium on Pre-Harvest Sprouting in Cereals.
Vancouver
1.
De Laethauwer S, Reheul D, De Riek J, Haesaert G. The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale. EUPHYTICA. 2009;168(3):379–84.
MLA
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, et al. “The Use of Vp1 in Real Time RT-PCR to Select for Pre-harvest Sprouting Tolerance in Triticale.” EUPHYTICA 168.3 (2009): 379–384. Print.