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The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale

Sarah De Laethauwer (UGent) , Dirk Reheul (UGent) , Jan De Riek and Geert Haesaert (UGent)
(2009) EUPHYTICA. 168(3). p.379-384
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Abstract
In spite of the availability of laboratory and field tests there is still a major problem to select pre-harvest sprouting (PHS) tolerant triticale varieties in a reliable, field-independent way. One approach to minimize the influence of environmental conditions and physio-morphological traits on PHS detection is using molecular genetic tools. The 'viviparous' Vp1 gene has been repeatedly described to play an important role in dormancy in wheat. A quantitative RT-PCR assay based on the expression of the Vp1 gene has been developed. Specific primers were designed for detecting Vp1 in both wheat and triticale. The expression levels of Vp1 were normalized using reference genes and relatively quantified with the comparative C-t-method. However, the first results indicate that the achieved Vp1 expression levels at 50 days post anthesis are not useful to select for PHS tolerance, both in wheat and triticale. This negative outcome so far is possibly due to the existence of several splicing events or to the late assaying moment in the kernel development, when Vp1 expression is found to be low.
Keywords
Triticale, Wheat, Vp1 gene, HOMOLOG, MAIZE, TRANSCRIPTS, EXPRESSION, CULTIVARS, VIVIPAROUS-1, DORMANCY, WHEAT, EMBRYO DEVELOPMENT, THUMB DWARFING GENE, Pre-harvest sprouting, Real time RT-PCR

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Chicago
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, and Geert Haesaert. 2009. “The Use of Vp1 in Real Time RT-PCR to Select for Pre-harvest Sprouting Tolerance in Triticale.” Euphytica 168 (3): 379–384.
APA
De Laethauwer, S., Reheul, D., De Riek, J., & Haesaert, G. (2009). The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale. EUPHYTICA, 168(3), 379–384. Presented at the 11th International Symposium on Pre-Harvest Sprouting in Cereals.
Vancouver
1.
De Laethauwer S, Reheul D, De Riek J, Haesaert G. The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale. EUPHYTICA. 2009;168(3):379–84.
MLA
De Laethauwer, Sarah, Dirk Reheul, Jan De Riek, et al. “The Use of Vp1 in Real Time RT-PCR to Select for Pre-harvest Sprouting Tolerance in Triticale.” EUPHYTICA 168.3 (2009): 379–384. Print.
@article{713652,
  abstract     = {In spite of the availability of laboratory and field tests there is still a major problem to select pre-harvest sprouting (PHS) tolerant triticale varieties in a reliable, field-independent way. One approach to minimize the influence of environmental conditions and physio-morphological traits on PHS detection is using molecular genetic tools. The 'viviparous' Vp1 gene has been repeatedly described to play an important role in dormancy in wheat. A quantitative RT-PCR assay based on the expression of the Vp1 gene has been developed. Specific primers were designed for detecting Vp1 in both wheat and triticale. The expression levels of Vp1 were normalized using reference genes and relatively quantified with the comparative C-t-method. However, the first results indicate that the achieved Vp1 expression levels at 50 days post anthesis are not useful to select for PHS tolerance, both in wheat and triticale. This negative outcome so far is possibly due to the existence of several splicing events or to the late assaying moment in the kernel development, when Vp1 expression is found to be low.},
  author       = {De Laethauwer, Sarah and Reheul, Dirk and De Riek, Jan and Haesaert, Geert},
  issn         = {1573-5060},
  journal      = {EUPHYTICA},
  keyword      = {Triticale,Wheat,Vp1 gene,HOMOLOG,MAIZE,TRANSCRIPTS,EXPRESSION,CULTIVARS,VIVIPAROUS-1,DORMANCY,WHEAT,EMBRYO DEVELOPMENT,THUMB DWARFING GENE,Pre-harvest sprouting,Real time RT-PCR},
  language     = {eng},
  location     = {Mendoza, Argentina},
  number       = {3},
  pages        = {379--384},
  title        = {The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale},
  url          = {http://dx.doi.org/10.1007/s10681-009-9930-7},
  volume       = {168},
  year         = {2009},
}

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