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Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/human b2 microglobulin Transgenic Rats (B27-rats)

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Abstract
Background: Although the association of the MHC class I allele HLA-B27 with Spondyloarthropathy (SpA) has been known for almost 35 years different hypotheses on its relation to disease mechanism still exist in parallel. Several lines of rats transgenic for HLA-B27 and human β2-microglobulin develop an inflammatory disease that strikingly resembles human SpA. It is hypothesized that disease in HLA-B27-transgenic rats arises as a consequence of interaction between antigen-presenting cells expressing high levels of HLA-B27 and peripheral T lymphocytes, and may result from a rupture of tolerance towards gut bacteria. Methods: We used 2D PAGE and iTRAQ to compare the protein expression profile of HLA-B27 dendritic cells (DCs) to that of healthy HLA-B7 expressing and nontransgenic (NTG) rat DCs. MHC II surface expression and apoptotic sensitivity were quantified using flow cytometry. Results: Three protein sets from the proteome analysis were indicative for aberrant cellular processes. First, all proteins involved in protein processing and MHC I assembly were upregulated in B27 DCs, illustrating the higher pressure on the ER due to misfolding of the HLA-B27 heavy chain. Second, all proteins directly influencing actin-dynamics were downregulated. We showed earlier that this not only influences motility, but also plays an important role in deficient immunological synapse formation. Third, the key thiol protease Cathepsin S involved in MHC II synthesis was downregulated, which led us to quantify RT1-B and RT1-D surface expression. Downregulation concerned both CD4+ and CD4- OX62+ HLA-B27 DC subpopulations and maturation enlarged differences in both population bias and expression intensity. Deficient actin dynamics could also contribute to this lower MHC II surface expression. Study of sensitivity to MHC class II-mediated apoptosis by antibody stimulation showed that compared to NTG, both B7 and B27 CD4+ DC were more prone to apoptosis but did not mutually differ. In contrast, overnight culturing resulted in a higher cell death in B27 than in control CD4- DC, even without antibody stimulation. Interestingly, decreased actin dynamics could also be involved in DC apoptosis. Conclusions: We have demonstrated that DCs are a very vulnerable cell type in HLA-B27 rats. Deficient cytoskeletal dynamics could immobilize matured DC in the tissue or induce aberrant migration patterns upon activation. On top of that abnormal intracellular trafficking and membrane organization together with a reduced expression of MHC class II molecules makes them aberrant in T-cell communication by deficient immunological synapse formation. Especially the reduced motility and viability of the tolerigenic CD4- DC could play an important role in initiating a systemic auto-immune response.

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MLA
Dhaenens, Maarten, et al. “Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/Human B2 Microglobulin Transgenic Rats (B27-Rats).” CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, vol. 26, no. 4, 2008, pp. 730–31.
APA
Dhaenens, M., Fert, I., Glatigny, S., Haerinck, S., Hacquard-Bouder, C., André, C., … Breban, M. (2008). Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/human b2 microglobulin Transgenic Rats (B27-rats). CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 26(4), 730–731. Italy.
Chicago author-date
Dhaenens, Maarten, Ingrid Fert, Simon Glatigny, Saskia Haerinck, Celine Hacquard-Bouder, Claudine André, Dirk Elewaut, Dieter Deforce, and Maxime Breban. 2008. “Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/Human B2 Microglobulin Transgenic Rats (B27-Rats).” In CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 26:730–31. Italy.
Chicago author-date (all authors)
Dhaenens, Maarten, Ingrid Fert, Simon Glatigny, Saskia Haerinck, Celine Hacquard-Bouder, Claudine André, Dirk Elewaut, Dieter Deforce, and Maxime Breban. 2008. “Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/Human B2 Microglobulin Transgenic Rats (B27-Rats).” In CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 26:730–731. Italy.
Vancouver
1.
Dhaenens M, Fert I, Glatigny S, Haerinck S, Hacquard-Bouder C, André C, et al. Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/human b2 microglobulin Transgenic Rats (B27-rats). In: CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. Italy; 2008. p. 730–1.
IEEE
[1]
M. Dhaenens et al., “Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/human b2 microglobulin Transgenic Rats (B27-rats),” in CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, Boston, 2008, vol. 26, no. 4, pp. 730–731.
@inproceedings{711121,
  abstract     = {{Background: Although the association of the MHC class I allele HLA-B27 with Spondyloarthropathy (SpA) has been known for almost 35 years different hypotheses on its relation to disease mechanism still exist in parallel. Several lines of rats transgenic for HLA-B27 and human β2-microglobulin develop an inflammatory disease that strikingly resembles human SpA. It is hypothesized that disease in HLA-B27-transgenic rats arises as a consequence of interaction between antigen-presenting cells expressing high levels of HLA-B27 and peripheral T lymphocytes, and may result from a rupture of tolerance towards gut bacteria.
Methods: We used 2D PAGE and iTRAQ to compare the protein expression profile of HLA-B27 dendritic cells (DCs) to that of healthy HLA-B7 expressing and nontransgenic (NTG) rat DCs. MHC II surface expression and apoptotic sensitivity were quantified using flow cytometry. 
Results: Three protein sets from the proteome analysis were indicative for aberrant cellular processes. First, all proteins involved in protein processing and MHC I assembly were upregulated in B27 DCs, illustrating the higher pressure on the ER due to misfolding of the HLA-B27 heavy chain. Second, all proteins directly influencing actin-dynamics were downregulated. We showed earlier that this not only influences motility, but also plays an important role in deficient immunological synapse formation.
Third, the key thiol protease Cathepsin S involved in MHC II synthesis was downregulated, which led us to quantify RT1-B and RT1-D surface expression. Downregulation concerned both CD4+ and CD4- OX62+ HLA-B27 DC subpopulations and maturation enlarged differences in both population bias and expression intensity. Deficient actin dynamics could also contribute to this lower MHC II surface expression. Study of sensitivity to MHC class II-mediated apoptosis by antibody stimulation showed that compared to NTG, both B7 and B27 CD4+ DC were more prone to apoptosis but did not mutually differ. In contrast, overnight culturing resulted in a higher cell death in B27 than in control CD4- DC, even without antibody stimulation. Interestingly, decreased actin dynamics could also be involved in DC apoptosis.
Conclusions: We have demonstrated that DCs are a very vulnerable cell type in HLA-B27 rats. Deficient cytoskeletal dynamics could immobilize matured DC in the tissue or induce aberrant migration patterns upon activation. On top of that abnormal intracellular trafficking and membrane organization together with a reduced expression of MHC class II molecules makes them aberrant in T-cell communication by deficient immunological synapse formation. Especially the reduced motility and viability of the tolerigenic CD4- DC could play an important role in initiating a systemic auto-immune response.}},
  author       = {{Dhaenens, Maarten and Fert, Ingrid and Glatigny, Simon and Haerinck, Saskia and Hacquard-Bouder, Celine and André, Claudine and Elewaut, Dirk and Deforce, Dieter and Breban, Maxime}},
  booktitle    = {{CLINICAL AND EXPERIMENTAL RHEUMATOLOGY}},
  issn         = {{0392-856X}},
  language     = {{eng}},
  location     = {{Boston}},
  number       = {{4}},
  pages        = {{730--731}},
  title        = {{Evidence for Altered Cytoskeleton Mobilization Pathway in Splenic Dendritic Cells (DC) from HLA-B27/human b2 microglobulin Transgenic Rats (B27-rats)}},
  volume       = {{26}},
  year         = {{2008}},
}