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Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice

(2009) JOURNAL OF CLINICAL INVESTIGATION. 119(6). p.1626-1637
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Abstract
Liver X receptors (LXRs) are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. However, exactly how LXRs modulate inflammation during infection remains unknown. To explore this, we used a mouse model of Mycobacterium tuberculosis infection. Upon intratracheal infection with M. tuberculosis, LXRs and LXR target genes were induced in CD11c(+) lung and alveolar cells. Furthermore, mice deficient in both LXR isoforms, LXR alpha and LXR beta (Lxra(-/-)Lxrb(-/-) mice), were more susceptible to infection, developing higher bacterial burdens and an increase in the size and number of granulomatous lesions. Interestingly, mice solely deficient in LXR alpha, but not those lacking only LXR beta, mirrored the susceptibility of the Lxra(-/-)Lxrb(-/-) animals. Lxra(-/-)Lxrb(-/-) mice failed to mount an effective early neutrophilic airway response to infection and showed dysregulation of both pro- and and inflammatory factors in CD11c(+) lung cells. T cell responses were strongly affected in Lxra(-/-)Lxrb(-/-) mice, showing near-complete abrogation of the infection-induced Th1 function - and even more so Th17 fimction - in the lungs. Treatment of WT mice with the LXR agonists TO901317 and GW3965 resulted in a 10-fold decrease of the pulmonary bacterial burden and a comparable increase of Th1/Th17 function in the lungs. The dependence of LXR signaling on the neutrophil IL-17 axis represents what we believe to be a novel function for these nuclear receptors in resistance to M. tuberculosis infection and may provide a new target for therapeutics.

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Chicago
Korf, Hannelie, Seppe Vander Beken, Marta Romano, Knut Steffensen, Benoit Stijlemans, Jan-Ake Gustafsson, Johan Grooten, and Kris Huygen. 2009. “Liver X Receptors Contribute to the Protective Immune Response Against Mycobacterium Tuberculosis in Mice.” Journal of Clinical Investigation 119 (6): 1626–1637.
APA
Korf, H., Vander Beken, S., Romano, M., Steffensen, K., Stijlemans, B., Gustafsson, J.-A., Grooten, J., et al. (2009). Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice. JOURNAL OF CLINICAL INVESTIGATION, 119(6), 1626–1637.
Vancouver
1.
Korf H, Vander Beken S, Romano M, Steffensen K, Stijlemans B, Gustafsson J-A, et al. Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice. JOURNAL OF CLINICAL INVESTIGATION. 2009;119(6):1626–37.
MLA
Korf, Hannelie, Seppe Vander Beken, Marta Romano, et al. “Liver X Receptors Contribute to the Protective Immune Response Against Mycobacterium Tuberculosis in Mice.” JOURNAL OF CLINICAL INVESTIGATION 119.6 (2009): 1626–1637. Print.
@article{710550,
  abstract     = {Liver X receptors (LXRs) are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. However, exactly how LXRs modulate inflammation during infection remains unknown. To explore this, we used a mouse model of Mycobacterium tuberculosis infection. Upon intratracheal infection with M. tuberculosis, LXRs and LXR target genes were induced in CD11c(+) lung and alveolar cells. Furthermore, mice deficient in both LXR isoforms, LXR alpha and LXR beta (Lxra(-/-)Lxrb(-/-) mice), were more susceptible to infection, developing higher bacterial burdens and an increase in the size and number of granulomatous lesions. Interestingly, mice solely deficient in LXR alpha, but not those lacking only LXR beta, mirrored the susceptibility of the Lxra(-/-)Lxrb(-/-) animals. Lxra(-/-)Lxrb(-/-) mice failed to mount an effective early neutrophilic airway response to infection and showed dysregulation of both pro- and and inflammatory factors in CD11c(+) lung cells. T cell responses were strongly affected in Lxra(-/-)Lxrb(-/-) mice, showing near-complete abrogation of the infection-induced Th1 function - and even more so Th17 fimction - in the lungs. Treatment of WT mice with the LXR agonists TO901317 and GW3965 resulted in a 10-fold decrease of the pulmonary bacterial burden and a comparable increase of Th1/Th17 function in the lungs. The dependence of LXR signaling on the neutrophil IL-17 axis represents what we believe to be a novel function for these nuclear receptors in resistance to M. tuberculosis infection and may provide a new target for therapeutics.},
  author       = {Korf, Hannelie and Vander Beken, Seppe and Romano, Marta and Steffensen, Knut and Stijlemans, Benoit and Gustafsson, Jan-Ake and Grooten, Johan and Huygen, Kris},
  issn         = {0021-9738},
  journal      = {JOURNAL OF CLINICAL INVESTIGATION},
  language     = {eng},
  number       = {6},
  pages        = {1626--1637},
  title        = {Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice},
  url          = {http://dx.doi.org/10.1172/JCI35288},
  volume       = {119},
  year         = {2009},
}

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