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Biotech rice : current developments and future detection challenges in food and feed chain

Marie-Alice Fraiture, Nancy Roosens, Isabel Taverniers, Marc De Loose UGent, Dieter Deforce UGent and Philippe Herman (2016) TRENDS IN FOOD SCIENCE & TECHNOLOGY. 52. p.66-79
abstract
Background: To improve agricultural practices and the food/feed security, plant breeding techniques were developed, including transgenesis commonly using Agrobacterium tumefaciens or biolistic technologies. To guarantee the traceability of GMO in food/feed chain and the consumer's freedom of choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and expensive regarding the number of analysis to perform. Moreover, the dispersion of publicly available information about developed GMO prevents to accurately estimate the efficiency of the standard detection system applied to unauthorized GMO. Scope and approach: To illustrate this problem, the case of rice, one of the leading staple crops, was investigated. An overview of worldwide developed biotech rice generated by transgenesis was thus conducted, based on 1067 peer-reviewed publications, and analysed regarding inter alia their expressed genes of interest and the corresponding traits, their transformation processes and the elements composing their transgenic cassettes. From this work, the power and weakness of the standard detection system, notably used by the European enforcement laboratories, are evaluated. To strengthen this system, especially with unauthorized GMO, additional strategies are suggested. Moreover, given the growing interest for biotech rice produced by new plant breeding techniques, related challenges for their detection are discussed. Key findings and conclusions: According to all collected information, suitable detection strategies, combining qPCR to additional technologies (e.g., DNA walking and NGS), are proposed to cover most of inventoried biotech rice. The present approach, including the data centralization to subsequently suggest appropriated detection strategies, can be extended to biotech events from different species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
Transgenic, Rice, Detection, Biotech crop, GENETICALLY-MODIFIED RICE, ORYZA-SATIVA L., FUNGUS MAGNAPORTHE-GRISEA, REAL-TIME PCR, MEDIATED ISOTHERMAL AMPLIFICATION, SHOW ENHANCED RESISTANCE, HERBICIDE-TOLERANT RICE, INCREASES GRAIN-YIELD, TRANSGENIC RICE, BLAST FUNGUS
journal title
TRENDS IN FOOD SCIENCE & TECHNOLOGY
Trends Food Sci. Technol.
volume
52
pages
66 - 79
Web of Science type
Review
Web of Science id
000377316500006
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
5.191 (2016)
JCR rank
4/129 (2016)
JCR quartile
1 (2016)
ISSN
0924-2244
DOI
10.1016/j.tifs.2016.03.011
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
7105457
handle
http://hdl.handle.net/1854/LU-7105457
date created
2016-02-29 10:30:35
date last changed
2017-06-12 10:48:05
@article{7105457,
  abstract     = {Background: To improve agricultural practices and the food/feed security, plant breeding techniques were developed, including transgenesis commonly using Agrobacterium tumefaciens or biolistic technologies. To guarantee the traceability of GMO in food/feed chain and the consumer's freedom of choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and expensive regarding the number of analysis to perform. Moreover, the dispersion of publicly available information about developed GMO prevents to accurately estimate the efficiency of the standard detection system applied to unauthorized GMO. 
Scope and approach: To illustrate this problem, the case of rice, one of the leading staple crops, was investigated. An overview of worldwide developed biotech rice generated by transgenesis was thus conducted, based on 1067 peer-reviewed publications, and analysed regarding inter alia their expressed genes of interest and the corresponding traits, their transformation processes and the elements composing their transgenic cassettes. From this work, the power and weakness of the standard detection system, notably used by the European enforcement laboratories, are evaluated. To strengthen this system, especially with unauthorized GMO, additional strategies are suggested. Moreover, given the growing interest for biotech rice produced by new plant breeding techniques, related challenges for their detection are discussed. 
Key findings and conclusions: According to all collected information, suitable detection strategies, combining qPCR to additional technologies (e.g., DNA walking and NGS), are proposed to cover most of inventoried biotech rice. The present approach, including the data centralization to subsequently suggest appropriated detection strategies, can be extended to biotech events from different species.},
  author       = {Fraiture, Marie-Alice and Roosens, Nancy and Taverniers, Isabel and De Loose, Marc and Deforce, Dieter and Herman, Philippe},
  issn         = {0924-2244},
  journal      = {TRENDS IN FOOD SCIENCE \& TECHNOLOGY},
  keyword      = {Transgenic,Rice,Detection,Biotech crop,GENETICALLY-MODIFIED RICE,ORYZA-SATIVA L.,FUNGUS MAGNAPORTHE-GRISEA,REAL-TIME PCR,MEDIATED ISOTHERMAL AMPLIFICATION,SHOW ENHANCED RESISTANCE,HERBICIDE-TOLERANT RICE,INCREASES GRAIN-YIELD,TRANSGENIC RICE,BLAST FUNGUS},
  language     = {eng},
  pages        = {66--79},
  title        = {Biotech rice : current developments and future detection challenges in food and feed chain},
  url          = {http://dx.doi.org/10.1016/j.tifs.2016.03.011},
  volume       = {52},
  year         = {2016},
}

Chicago
Fraiture, Marie-Alice, Nancy Roosens, Isabel Taverniers, Marc De Loose, Dieter Deforce, and Philippe Herman. 2016. “Biotech Rice : Current Developments and Future Detection Challenges in Food and Feed Chain.” Trends in Food Science & Technology 52: 66–79.
APA
Fraiture, M.-A., Roosens, N., Taverniers, I., De Loose, M., Deforce, D., & Herman, P. (2016). Biotech rice : current developments and future detection challenges in food and feed chain. TRENDS IN FOOD SCIENCE & TECHNOLOGY, 52, 66–79.
Vancouver
1.
Fraiture M-A, Roosens N, Taverniers I, De Loose M, Deforce D, Herman P. Biotech rice : current developments and future detection challenges in food and feed chain. TRENDS IN FOOD SCIENCE & TECHNOLOGY. 2016;52:66–79.
MLA
Fraiture, Marie-Alice, Nancy Roosens, Isabel Taverniers, et al. “Biotech Rice : Current Developments and Future Detection Challenges in Food and Feed Chain.” TRENDS IN FOOD SCIENCE & TECHNOLOGY 52 (2016): 66–79. Print.