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A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours

(2015) JOURNAL OF GENERAL VIROLOGY. 96(9). p.2764-2768
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Abstract
Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.
Keywords
VACCINATION, HORSES, TRANSFORMATION, DNA, MHC CLASS-I, MOUSE, TYPE-1, RNA

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Citation

Please use this url to cite or link to this publication:

Chicago
Bogaert, Lies, Andrew W Woodham, Diane M Da Silva, Ann Martens, Evelyne Meyer, and W Martin Kast. 2015. “A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours.” Journal of General Virology 96 (9): 2764–2768.
APA
Bogaert, Lies, Woodham, A. W., Da Silva, D. M., Martens, A., Meyer, E., & Kast, W. M. (2015). A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours. JOURNAL OF GENERAL VIROLOGY, 96(9), 2764–2768.
Vancouver
1.
Bogaert L, Woodham AW, Da Silva DM, Martens A, Meyer E, Kast WM. A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours. JOURNAL OF GENERAL VIROLOGY. 2015;96(9):2764–8.
MLA
Bogaert, Lies et al. “A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours.” JOURNAL OF GENERAL VIROLOGY 96.9 (2015): 2764–2768. Print.
@article{7094684,
  abstract     = {Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.},
  author       = {Bogaert, Lies and Woodham, Andrew W and Da Silva, Diane M and Martens, Ann and Meyer, Evelyne and Kast, W Martin},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keywords     = {VACCINATION,HORSES,TRANSFORMATION,DNA,MHC CLASS-I,MOUSE,TYPE-1,RNA},
  language     = {eng},
  number       = {9},
  pages        = {2764--2768},
  title        = {A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours},
  url          = {http://dx.doi.org/10.1099/vir.0.000212},
  volume       = {96},
  year         = {2015},
}

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