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Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle

Sofia N Karanikola, Jürgen Krücken, Sabrina Ramünke, Theo de Waal, Johan Höglund, Johannes Charlier UGent, Corinna Weber, Elisabeth Müller, Slawomir J Kowalczyk, Jaroslaw Kaba, et al. (2015) PARASITES & VECTORS. 8.
abstract
Background: A major constraint for the effective control and management of helminth parasites is the lack of rapid, high-throughput, routine diagnostic tests to assess the health status of individual animals and herds and to identify the parasite species responsible for these helminthoses. The capability of a multiplex platform for the simultaneous detection of three pasture associated parasite species was evaluated and compared to existing ELISAs. Methods: The recombinant antigens 14.2 kDa ES protein for Cooperia oncophora, major sperm protein for Dictyocaulus viviparus and Cathepsin L1 for Fasciola hepatica were recombinantly expressed either in Escherichia coli or Pichia pastoris. Antigens were covalently coupled onto magnetic beads. Optimal concentrations for coupling were determined following the examination of serum samples collected from experimentally mono-infected animals, before and after their infection with the target species. Absence of cross-reactivity was further determined with sera from calves mono-infected with Haemonchus contortus, Ostertagia ostertagi and Trichostrongylus colubriformis. Examination of negative serum samples was characterised by low median fluorescence intensity (MFI). Results: Establishment of the optimal serum dilution of 1:200 was achieved for all three bead sets. Receiver Operating Characteristic analyses were performed to obtain cut-off MFI values for each parasite separately. Sensitivity and specificity at the chosen cut-off values were close to, or 100 % for all bead sets. Examination of serum samples collected on different days post infection from different animals showed a high reproducibility of the assays. Serum samples were additionally examined with two already established ELISAs, an in-house ELISA using the recombinant MSP as an antigen and a DRG ELISA using Cathepsin L1 for liver fluke. The results between the assays were compared and kappa tests revealed an overall good agreement. Conclusions: A versatile bead-based assay using fluorescence detection (xMAP (R) technology) was developed to simultaneously detect antibodies against C. oncophora, D. viviparus and F. hepatica in cattle serum samples. This platform provides rapid, high-throughput results and is highly sensitive and specific in comparison to existing serological as well as coproscopical diagnostic techniques.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Cattle, Serum, Multiplex immunoassays, Luminex, Liver fluke, Lungworm, Parasitic gastroenteritis, Diagnosis, LINKED-IMMUNOSORBENT-ASSAY, FLOW-CYTOMETRIC ASSAY, ADULT DAIRY-COWS, SIMULTANEOUS QUANTITATION, MICROSPHERE IMMUNOASSAY, INFLAMMATORY CYTOKINES, XMAP(TM) TECHNOLOGY, LUNGWORM INFECTION, MOLECULAR METHOD, CATHEPSIN L1
journal title
PARASITES & VECTORS
Parasites Vectors
volume
8
article number
335
pages
11 pages
Web of Science type
Article
Web of Science id
000357469800001
JCR category
PARASITOLOGY
JCR impact factor
3.234 (2015)
JCR rank
8/36 (2015)
JCR quartile
1 (2015)
ISSN
1756-3305
DOI
10.1186/s13071-015-0924-0
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
7093789
handle
http://hdl.handle.net/1854/LU-7093789
date created
2016-02-21 21:52:07
date last changed
2017-04-05 09:15:58
@article{7093789,
  abstract     = {Background: A major constraint for the effective control and management of helminth parasites is the lack of rapid, high-throughput, routine diagnostic tests to assess the health status of individual animals and herds and to identify the parasite species responsible for these helminthoses. The capability of a multiplex platform for the simultaneous detection of three pasture associated parasite species was evaluated and compared to existing ELISAs. 
Methods: The recombinant antigens 14.2 kDa ES protein for Cooperia oncophora, major sperm protein for Dictyocaulus viviparus and Cathepsin L1 for Fasciola hepatica were recombinantly expressed either in Escherichia coli or Pichia pastoris. Antigens were covalently coupled onto magnetic beads. Optimal concentrations for coupling were determined following the examination of serum samples collected from experimentally mono-infected animals, before and after their infection with the target species. Absence of cross-reactivity was further determined with sera from calves mono-infected with Haemonchus contortus, Ostertagia ostertagi and Trichostrongylus colubriformis. Examination of negative serum samples was characterised by low median fluorescence intensity (MFI). 
Results: Establishment of the optimal serum dilution of 1:200 was achieved for all three bead sets. Receiver Operating Characteristic analyses were performed to obtain cut-off MFI values for each parasite separately. Sensitivity and specificity at the chosen cut-off values were close to, or 100 \% for all bead sets. Examination of serum samples collected on different days post infection from different animals showed a high reproducibility of the assays. Serum samples were additionally examined with two already established ELISAs, an in-house ELISA using the recombinant MSP as an antigen and a DRG ELISA using Cathepsin L1 for liver fluke. The results between the assays were compared and kappa tests revealed an overall good agreement. 
Conclusions: A versatile bead-based assay using fluorescence detection (xMAP (R) technology) was developed to simultaneously detect antibodies against C. oncophora, D. viviparus and F. hepatica in cattle serum samples. This platform provides rapid, high-throughput results and is highly sensitive and specific in comparison to existing serological as well as coproscopical diagnostic techniques.},
  articleno    = {335},
  author       = {Karanikola, Sofia N and Kr{\"u}cken, J{\"u}rgen and Ram{\"u}nke, Sabrina and de Waal, Theo and H{\"o}glund, Johan and Charlier, Johannes and Weber, Corinna and M{\"u}ller, Elisabeth and Kowalczyk, Slawomir J and Kaba, Jaroslaw and von Samson-Himmelstjerna, Georg and Demeler, Janina},
  issn         = {1756-3305},
  journal      = {PARASITES \& VECTORS},
  keyword      = {Cattle,Serum,Multiplex immunoassays,Luminex,Liver fluke,Lungworm,Parasitic gastroenteritis,Diagnosis,LINKED-IMMUNOSORBENT-ASSAY,FLOW-CYTOMETRIC ASSAY,ADULT DAIRY-COWS,SIMULTANEOUS QUANTITATION,MICROSPHERE IMMUNOASSAY,INFLAMMATORY CYTOKINES,XMAP(TM) TECHNOLOGY,LUNGWORM INFECTION,MOLECULAR METHOD,CATHEPSIN L1},
  language     = {eng},
  pages        = {11},
  title        = {Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle},
  url          = {http://dx.doi.org/10.1186/s13071-015-0924-0},
  volume       = {8},
  year         = {2015},
}

Chicago
Karanikola, Sofia N, Jürgen Krücken, Sabrina Ramünke, Theo de Waal, Johan Höglund, Johannes Charlier, Corinna Weber, et al. 2015. “Development of a Multiplex Fluorescence Immunological Assay for the Simultaneous Detection of Antibodies Against Cooperia Oncophora, Dictyocaulus Viviparus and Fasciola Hepatica in Cattle.” Parasites & Vectors 8.
APA
Karanikola, S. N., Krücken, J., Ramünke, S., de Waal, T., Höglund, J., Charlier, J., Weber, C., et al. (2015). Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle. PARASITES & VECTORS, 8.
Vancouver
1.
Karanikola SN, Krücken J, Ramünke S, de Waal T, Höglund J, Charlier J, et al. Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle. PARASITES & VECTORS. 2015;8.
MLA
Karanikola, Sofia N, Jürgen Krücken, Sabrina Ramünke, et al. “Development of a Multiplex Fluorescence Immunological Assay for the Simultaneous Detection of Antibodies Against Cooperia Oncophora, Dictyocaulus Viviparus and Fasciola Hepatica in Cattle.” PARASITES & VECTORS 8 (2015): n. pag. Print.