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Reducing compounds equivocally influence oxidation during digestion of a high-fat beef product, which promotes cytotoxicity colorectal carcinoma cell lines

Thomas Van Hecke (UGent) , An Wouters, Caroline Rombouts (UGent) , Tazkiyah Izzati (UGent) , Alberto Berardo (UGent) , Els Vossen (UGent) , Erik Claeys (UGent) , John Van Camp (UGent) , Katleen Raes (UGent) , Lynn Vanhaecke (UGent) , et al.
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Abstract
We studied the formation of malondialdehyde, 4-hydroxy-nonenal, and hexanal (lipid oxidation products, LOP) during in vitro digestion of a cooked low-fat and high-fat beef product in response to the addition of reducing compounds. We also investigated whether higher LOP in the digests resulted in a higher cyto- and genotoxicity in Caco-2, HT-29 and HCT-116 cell lines. High-fat compared to low-fat beef digests contained approximately 10-fold higher LOP concentrations (all P < 0.001), and induced higher cytotoxicity (P < 0.001). During digestion of the high-fat product, phenolic acids (gallic, ferulic, chlorogenic, and caffeic acid) displayed either pro-oxidant or antioxidant behavior at lower and higher doses respectively, whereas ascorbic acid was pro-oxidant at all doses, and the lipophilic reducing compounds (α-tocopherol, quercetin, and silibinin) all exerted a clear antioxidant effect. During digestion of the low-fat product, the hydrophilic compounds and quercetin were antioxidant. Decreases or increases in LOP concentrations amounted to 100% change versus controls.
Keywords
lipid oxidation, malondialdehyde, 4-hydroxy-nonenal, γ-H2AX assay, xCelligence, IN-VITRO DIGESTION, DENSITY-LIPOPROTEIN OXIDATION, SIMULATED GASTRIC DIGESTION, DNA ADDUCT FORMATION, LIPID-PEROXIDATION, COLON-CANCER, RED MEAT, PHENOLIC-COMPOUNDS, HEME-IRON, ANTIOXIDANT

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Chicago
Van Hecke, Thomas, An Wouters, Caroline Rombouts, Tazkiyah Izzati, Alberto Berardo, Els Vossen, Erik Claeys, et al. 2016. “Reducing Compounds Equivocally Influence Oxidation During Digestion of a High-fat Beef Product, Which Promotes Cytotoxicity Colorectal Carcinoma Cell Lines.” Journal of Agricultural and Food Chemistry 64 (7): 1600–1609.
APA
Van Hecke, Thomas, Wouters, A., Rombouts, C., Izzati, T., Berardo, A., Vossen, E., Claeys, E., et al. (2016). Reducing compounds equivocally influence oxidation during digestion of a high-fat beef product, which promotes cytotoxicity colorectal carcinoma cell lines. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 64(7), 1600–1609.
Vancouver
1.
Van Hecke T, Wouters A, Rombouts C, Izzati T, Berardo A, Vossen E, et al. Reducing compounds equivocally influence oxidation during digestion of a high-fat beef product, which promotes cytotoxicity colorectal carcinoma cell lines. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2016;64(7):1600–9.
MLA
Van Hecke, Thomas, An Wouters, Caroline Rombouts, et al. “Reducing Compounds Equivocally Influence Oxidation During Digestion of a High-fat Beef Product, Which Promotes Cytotoxicity Colorectal Carcinoma Cell Lines.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.7 (2016): 1600–1609. Print.
@article{7085500,
  abstract     = {We studied the formation of malondialdehyde, 4-hydroxy-nonenal, and hexanal (lipid oxidation products, LOP) during in vitro digestion of a cooked low-fat and high-fat beef product in response to the addition of reducing compounds. We also investigated whether higher LOP in the digests resulted in a higher cyto- and genotoxicity in Caco-2, HT-29 and HCT-116 cell lines. High-fat compared to low-fat beef digests contained approximately 10-fold higher LOP concentrations (all P {\textlangle} 0.001), and induced higher cytotoxicity (P {\textlangle} 0.001). During digestion of the high-fat product, phenolic acids (gallic, ferulic, chlorogenic, and caffeic acid) displayed either pro-oxidant or antioxidant behavior at lower and higher doses respectively, whereas ascorbic acid was pro-oxidant at all doses, and the lipophilic reducing compounds (\ensuremath{\alpha}-tocopherol, quercetin, and silibinin) all exerted a clear antioxidant effect. During digestion of the low-fat product, the hydrophilic compounds and quercetin were antioxidant. Decreases or increases in LOP concentrations amounted to 100\% change versus controls.},
  author       = {Van Hecke, Thomas and Wouters, An and Rombouts, Caroline and Izzati, Tazkiyah and Berardo, Alberto and Vossen, Els and Claeys, Erik and Van Camp, John and Raes, Katleen and Vanhaecke, Lynn and Peeters, Marc and De Vos, Winnok and De Smet, Stefaan},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {lipid oxidation,malondialdehyde,4-hydroxy-nonenal,\ensuremath{\gamma}-H2AX assay,xCelligence,IN-VITRO DIGESTION,DENSITY-LIPOPROTEIN OXIDATION,SIMULATED GASTRIC DIGESTION,DNA ADDUCT FORMATION,LIPID-PEROXIDATION,COLON-CANCER,RED MEAT,PHENOLIC-COMPOUNDS,HEME-IRON,ANTIOXIDANT},
  language     = {eng},
  number       = {7},
  pages        = {1600--1609},
  title        = {Reducing compounds equivocally influence oxidation during digestion of a high-fat beef product, which promotes cytotoxicity colorectal carcinoma cell lines},
  url          = {http://dx.doi.org/10.1021/acs.jafc.5b05915},
  volume       = {64},
  year         = {2016},
}

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