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Variable protection against experimental broiler necrotic enteritis after immunisation with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant

(2016) AVIAN PATHOLOGY. 45(3). p.381-388
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Abstract
Necrotic enteritis toxin B (NetB) is a pore-forming toxin produced by Clostridium perfringens and has been shown to play a key role in avian necrotic enteritis (NE), a disease causing significant costs to the poultry production industry worldwide. The aim of this work was to determine whether immunisation with a non-toxic variant of NetB (NetB W262A) and the C-terminal fragment of C. perfringens alpha-toxin (CPA247–370) would provide protection against experimental NE. Immunised animals with either antigen or a combination of antigens developed serum antibody levels against NetB and CPA. When CPA247–370 and NetB W262A were used in combination as immunogens, an increased protection was observed after oral challenge by individual dosing, but not after in-feed challenge.
Keywords
Clostridium perfringens, necrotic enteritis, NetB, alpha-toxin, immunization, CHICKENS, VACCINATION, PERFORMANCE, FOWL, COCCIDIOSIS, CHALLENGE, INFECTION, RESPONSES, PROTEINS, ANTIGENS

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Citation

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MLA
Fernandes da Costa, Sergio, Dorien Mot, Sofie Geeraerts, et al. “Variable Protection Against Experimental Broiler Necrotic Enteritis After Immunisation with the C-terminal Fragment of Clostridium Perfringens Alpha-toxin and a Non-toxic NetB Variant.” AVIAN PATHOLOGY 45.3 (2016): 381–388. Print.
APA
Fernandes da Costa, S., Mot, D., Geeraerts, S., Bokori-Brown, M., Van Immerseel, F., & Titball, R. (2016). Variable protection against experimental broiler necrotic enteritis after immunisation with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant. AVIAN PATHOLOGY, 45(3), 381–388.
Chicago author-date
Fernandes da Costa, Sergio, Dorien Mot, Sofie Geeraerts, Monika Bokori-Brown, Filip Van Immerseel, and Richard Titball. 2016. “Variable Protection Against Experimental Broiler Necrotic Enteritis After Immunisation with the C-terminal Fragment of Clostridium Perfringens Alpha-toxin and a Non-toxic NetB Variant.” Avian Pathology 45 (3): 381–388.
Chicago author-date (all authors)
Fernandes da Costa, Sergio, Dorien Mot, Sofie Geeraerts, Monika Bokori-Brown, Filip Van Immerseel, and Richard Titball. 2016. “Variable Protection Against Experimental Broiler Necrotic Enteritis After Immunisation with the C-terminal Fragment of Clostridium Perfringens Alpha-toxin and a Non-toxic NetB Variant.” Avian Pathology 45 (3): 381–388.
Vancouver
1.
Fernandes da Costa S, Mot D, Geeraerts S, Bokori-Brown M, Van Immerseel F, Titball R. Variable protection against experimental broiler necrotic enteritis after immunisation with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant. AVIAN PATHOLOGY. 2016;45(3):381–8.
IEEE
[1]
S. Fernandes da Costa, D. Mot, S. Geeraerts, M. Bokori-Brown, F. Van Immerseel, and R. Titball, “Variable protection against experimental broiler necrotic enteritis after immunisation with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant,” AVIAN PATHOLOGY, vol. 45, no. 3, pp. 381–388, 2016.
@article{7084396,
  abstract     = {Necrotic enteritis toxin B (NetB) is a pore-forming toxin produced by Clostridium perfringens and has been shown to play a key role in avian necrotic enteritis (NE), a disease causing significant costs to the poultry production industry worldwide. The aim of this work was to determine whether immunisation with a non-toxic variant of NetB (NetB W262A) and the C-terminal fragment of C. perfringens alpha-toxin (CPA247–370) would provide protection against experimental NE. Immunised animals with either antigen or a combination of antigens developed serum antibody levels against NetB and CPA. When CPA247–370 and NetB W262A were used in combination as immunogens, an increased protection was observed after oral challenge by individual dosing, but not after in-feed challenge.},
  author       = {Fernandes da Costa, Sergio and Mot, Dorien and Geeraerts, Sofie and Bokori-Brown, Monika and Van Immerseel, Filip and Titball, Richard},
  issn         = {0307-9457},
  journal      = {AVIAN PATHOLOGY},
  keywords     = {Clostridium perfringens,necrotic enteritis,NetB,alpha-toxin,immunization,CHICKENS,VACCINATION,PERFORMANCE,FOWL,COCCIDIOSIS,CHALLENGE,INFECTION,RESPONSES,PROTEINS,ANTIGENS},
  language     = {eng},
  number       = {3},
  pages        = {381--388},
  title        = {Variable protection against experimental broiler necrotic enteritis after immunisation with the C-terminal fragment of Clostridium perfringens alpha-toxin and a non-toxic NetB variant},
  url          = {http://dx.doi.org/10.1080/03079457.2015.1129663},
  volume       = {45},
  year         = {2016},
}

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