Advanced search
1 file | 2.55 MB Add to list

Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles

Vikram Virdi (UGent) , Paloma Juarez Ortega (UGent) , Véronique Boudolf (UGent) and Anna Depicker (UGent)
Author
Organization
Abstract
Vaccination is a successful strategy to proactively develop immunity to a certain pathogen, but most vaccines fail to trigger a specific immune response at the mucosal surfaces, which are the first port of entry for infectious agents. At the mucosal surfaces, the predominant immunoglobulin is secretory IgA (SIgA) that specifically neutralizes viruses and prevents bacterial colonization. Mucosal passive immunization, i.e. the application of pathogen-specific SIgAs at the mucosae, can be an effective alternative to achieve mucosal protection. However, this approach is not straightforward, mainly because SIgAs are difficult to obtain from convalescent sources, while recombinant SIgA production is challenging due to its complex structure. This review provides an overview of manufacturing difficulties presented by the unique structural diversity of SIgAs, and the innovative solutions being explored for SIgA production in mammalian and plant expression systems.
Keywords
PROTEIN-L, POLYMERIC IGA, PLANT EXPRESSION, EXPRESSION SYSTEMS, ANTIBODY-PRODUCTION, INFECTIOUS-DISEASES, AFFINITY-CHROMATOGRAPHY, Topical application, Immune prophylaxis, JACKFRUIT LECTIN, SECRETORY IMMUNOGLOBULIN-A, ANTIGEN-BINDING, Molecular farming, IgA purification, Mucosal infections, Plant-based production

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.55 MB

Citation

Please use this url to cite or link to this publication:

MLA
Virdi, Vikram et al. “Recombinant IgA Production for Mucosal Passive Immunization, Advancing Beyond the Hurdles.” CELLULAR AND MOLECULAR LIFE SCIENCES 73.3 (2016): 535–545. Print.
APA
Virdi, V., Juarez Ortega, P., Boudolf, V., & Depicker, A. (2016). Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles. CELLULAR AND MOLECULAR LIFE SCIENCES, 73(3), 535–545.
Chicago author-date
Virdi, Vikram, Paloma Juarez Ortega, Véronique Boudolf, and Anna Depicker. 2016. “Recombinant IgA Production for Mucosal Passive Immunization, Advancing Beyond the Hurdles.” Cellular and Molecular Life Sciences 73 (3): 535–545.
Chicago author-date (all authors)
Virdi, Vikram, Paloma Juarez Ortega, Véronique Boudolf, and Anna Depicker. 2016. “Recombinant IgA Production for Mucosal Passive Immunization, Advancing Beyond the Hurdles.” Cellular and Molecular Life Sciences 73 (3): 535–545.
Vancouver
1.
Virdi V, Juarez Ortega P, Boudolf V, Depicker A. Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles. CELLULAR AND MOLECULAR LIFE SCIENCES. 2016;73(3):535–45.
IEEE
[1]
V. Virdi, P. Juarez Ortega, V. Boudolf, and A. Depicker, “Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles,” CELLULAR AND MOLECULAR LIFE SCIENCES, vol. 73, no. 3, pp. 535–545, 2016.
@article{7081955,
  abstract     = {Vaccination is a successful strategy to proactively develop immunity to a certain pathogen, but most vaccines fail to trigger a specific immune response at the mucosal surfaces, which are the first port of entry for infectious agents. At the mucosal surfaces, the predominant immunoglobulin is secretory IgA (SIgA) that specifically neutralizes viruses and prevents bacterial colonization. Mucosal passive immunization, i.e. the application of pathogen-specific SIgAs at the mucosae, can be an effective alternative to achieve mucosal protection. However, this approach is not straightforward, mainly because SIgAs are difficult to obtain from convalescent sources, while recombinant SIgA production is challenging due to its complex structure. This review provides an overview of manufacturing difficulties presented by the unique structural diversity of SIgAs, and the innovative solutions being explored for SIgA production in mammalian and plant expression systems.},
  author       = {Virdi, Vikram and Juarez Ortega, Paloma and Boudolf, Véronique and Depicker, Anna},
  issn         = {1420-682X},
  journal      = {CELLULAR AND MOLECULAR LIFE SCIENCES},
  keywords     = {PROTEIN-L,POLYMERIC IGA,PLANT EXPRESSION,EXPRESSION SYSTEMS,ANTIBODY-PRODUCTION,INFECTIOUS-DISEASES,AFFINITY-CHROMATOGRAPHY,Topical application,Immune prophylaxis,JACKFRUIT LECTIN,SECRETORY IMMUNOGLOBULIN-A,ANTIGEN-BINDING,Molecular farming,IgA purification,Mucosal infections,Plant-based production},
  language     = {eng},
  number       = {3},
  pages        = {535--545},
  title        = {Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles},
  url          = {http://dx.doi.org/10.1007/s00018-015-2074-0},
  volume       = {73},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: