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Lateral root inducible system in Arabidopsis and maize

Hanne Crombez (UGent) , Ianto Roberts (UGent) , Nick Vangheluwe (UGent) , Hans Motte (UGent) , Leentje Jansen (UGent) , Tom Beeckman (UGent) and Boris Parizot (UGent)
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Abstract
Lateral root development contributes significantly to the root system, and hence is crucial for plant growth. The study of lateral root initiation is however tedious, because it occurs only in a few cells inside the root and in an unpredictable manner. To circumvent this problem, a Lateral Root Inducible System (LRIS) has been developed. By treating seedlings consecutively with an auxin transport inhibitor and a synthetic auxin, highly controlled lateral root initiation occurs synchronously in the primary root, allowing abundant sampling of a desired developmental stage. The LRIS has first been developed for Arabidopsis thaliana, but can be applied to other plants as well. Accordingly, it has been adapted for use in maize (Zea mays). A detailed overview of the different steps of the LRIS in both plants is given. The combination of this system with comparative transcriptomics made it possible to identify functional homologs of Arabidopsis lateral root initiation genes in other species as illustrated here for the CYCLIN B1; 1 (CYCB1; 1) cell cycle gene in maize. Finally, the principles that need to be taken into account when an LRIS is developed for other plant species are discussed.
Keywords
ACID, TOOL, RECEPTOR, GENES, PERICYCLE, IDENTIFICATION, MICRODISSECTION, THALIANA, CELL-CYCLE, INITIATION, transcriptomics, Maize, Arabidopsis, Lateral Root Development, Lateral Root Initiation, LRIS, Lateral Root Inducible System, Plant Biology

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MLA
Crombez, Hanne et al. “Lateral Root Inducible System in Arabidopsis and Maize.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 107 (2016): n. pag. Print.
APA
Crombez, H., Roberts, I., Vangheluwe, N., Motte, H., Jansen, L., Beeckman, T., & Parizot, B. (2016). Lateral root inducible system in Arabidopsis and maize. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (107).
Chicago author-date
Crombez, Hanne, Ianto Roberts, Nick Vangheluwe, Hans Motte, Leentje Jansen, Tom Beeckman, and Boris Parizot. 2016. “Lateral Root Inducible System in Arabidopsis and Maize.” Jove-journal of Visualized Experiments (107).
Chicago author-date (all authors)
Crombez, Hanne, Ianto Roberts, Nick Vangheluwe, Hans Motte, Leentje Jansen, Tom Beeckman, and Boris Parizot. 2016. “Lateral Root Inducible System in Arabidopsis and Maize.” Jove-journal of Visualized Experiments (107).
Vancouver
1.
Crombez H, Roberts I, Vangheluwe N, Motte H, Jansen L, Beeckman T, et al. Lateral root inducible system in Arabidopsis and maize. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2016;(107).
IEEE
[1]
H. Crombez et al., “Lateral root inducible system in Arabidopsis and maize,” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no. 107, 2016.
@article{7081600,
  abstract     = {Lateral root development contributes significantly to the root system, and hence is crucial for plant growth. The study of lateral root initiation is however tedious, because it occurs only in a few cells inside the root and in an unpredictable manner. To circumvent this problem, a Lateral Root Inducible System (LRIS) has been developed. By treating seedlings consecutively with an auxin transport inhibitor and a synthetic auxin, highly controlled lateral root initiation occurs synchronously in the primary root, allowing abundant sampling of a desired developmental stage. The LRIS has first been developed for Arabidopsis thaliana, but can be applied to other plants as well. Accordingly, it has been adapted for use in maize (Zea mays). A detailed overview of the different steps of the LRIS in both plants is given. The combination of this system with comparative transcriptomics made it possible to identify functional homologs of Arabidopsis lateral root initiation genes in other species as illustrated here for the CYCLIN B1; 1 (CYCB1; 1) cell cycle gene in maize. Finally, the principles that need to be taken into account when an LRIS is developed for other plant species are discussed.},
  articleno    = {e53481},
  author       = {Crombez, Hanne and Roberts, Ianto and Vangheluwe, Nick and Motte, Hans and Jansen, Leentje and Beeckman, Tom and Parizot, Boris},
  issn         = {1940-087X},
  journal      = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS},
  keywords     = {ACID,TOOL,RECEPTOR,GENES,PERICYCLE,IDENTIFICATION,MICRODISSECTION,THALIANA,CELL-CYCLE,INITIATION,transcriptomics,Maize,Arabidopsis,Lateral Root Development,Lateral Root Initiation,LRIS,Lateral Root Inducible System,Plant Biology},
  language     = {eng},
  number       = {107},
  pages        = {9},
  title        = {Lateral root inducible system in Arabidopsis and maize},
  url          = {http://dx.doi.org/10.3791/53481},
  year         = {2016},
}

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