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Strigolactones as an auxiliary hormonal defence mechanism against leafy gall syndrome in Arabidopsis thaliana

(2015) JOURNAL OF EXPERIMENTAL BOTANY. 66(16). p.5123-5134
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Abstract
Leafy gall syndrome is the consequence of modified plant development in response to a mixture of cytokinins secreted by the biotrophic actinomycete Rhodococcus fascians. The similarity of the induced symptoms with the phenotype of plant mutants defective in strigolactone biosynthesis and signalling prompted an evaluation of the involvement of strigolactones in this pathology. All tested strigolactone-related Arabidopsis thallana mutants were hypersensitive to R. fascians. Moreover, treatment with the synthetic strigolactone mixture GR24 and with the carotenoid cleavage dioxygenase inhibitor D2 illustrated that strigolactones acted as antagonistic compounds that restricted the morphogenic activity of R. fascians. Transcript profiling of the MORE ILLARY GROWTH1 (M 1), M, M 3, M, 4, and BRANCHED1 (BRC1) genes in the wild-type Columbia-0 accession and in different mutant backgrounds revealed that upregulation of strigolactone biosynthesis genes was triggered indirectly by the bacterial cytokinins via host-derived auxin and led to the activation of BRC1 expression, inhibiting the outgrowth of the newly developing shoots, a typical hallmark of leafy gall syndrome. Taken together, these data support the emerging insight that balances are critical for optimal leafy gall development: the long-lasting biotrophic interaction is possible only because the host activates a set of countermeasures including the strigolactone response in reaction to bacterial cytokinins to constrain the activity of R. fascians.
Keywords
ANALOG GR24, RESPONSES, BUD OUTGROWTH, ACTS DOWNSTREAM, SIGNAL INTEGRATION, SYMPTOM DEVELOPMENT, MEDICAGO-TRUNCATULA, BOX PROTEIN MAX2, PLANT DEVELOPMENT, RHODOCOCCUS-FASCIANS INFECTION, witches' broom, Gram-positive phytopathogen, Apical dominance

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Chicago
Stes, Elisabeth, Stephen Depuydt, Annick De Keyser, Cedrick Matthys, Kris Audenaert, Koichi Yoneyama, Stefaan Werbrouck, Sofie Goormachtig, and Danny Vereecke. 2015. “Strigolactones as an Auxiliary Hormonal Defence Mechanism Against Leafy Gall Syndrome in Arabidopsis Thaliana.” Journal of Experimental Botany 66 (16): 5123–5134.
APA
Stes, E., Depuydt, S., De Keyser, A., Matthys, C., Audenaert, K., Yoneyama, K., Werbrouck, S., et al. (2015). Strigolactones as an auxiliary hormonal defence mechanism against leafy gall syndrome in Arabidopsis thaliana. JOURNAL OF EXPERIMENTAL BOTANY, 66(16), 5123–5134.
Vancouver
1.
Stes E, Depuydt S, De Keyser A, Matthys C, Audenaert K, Yoneyama K, et al. Strigolactones as an auxiliary hormonal defence mechanism against leafy gall syndrome in Arabidopsis thaliana. JOURNAL OF EXPERIMENTAL BOTANY. 2015;66(16):5123–34.
MLA
Stes, Elisabeth, Stephen Depuydt, Annick De Keyser, et al. “Strigolactones as an Auxiliary Hormonal Defence Mechanism Against Leafy Gall Syndrome in Arabidopsis Thaliana.” JOURNAL OF EXPERIMENTAL BOTANY 66.16 (2015): 5123–5134. Print.
@article{7081537,
  abstract     = {Leafy gall syndrome is the consequence of modified plant development in response to a mixture of cytokinins secreted by the biotrophic actinomycete Rhodococcus fascians. The similarity of the induced symptoms with the phenotype of plant mutants defective in strigolactone biosynthesis and signalling prompted an evaluation of the involvement of strigolactones in this pathology. All tested strigolactone-related Arabidopsis thallana mutants were hypersensitive to R. fascians. Moreover, treatment with the synthetic strigolactone mixture GR24 and with the carotenoid cleavage dioxygenase inhibitor D2 illustrated that strigolactones acted as antagonistic compounds that restricted the morphogenic activity of R. fascians. Transcript profiling of the MORE ILLARY GROWTH1 (M 1), M, M 3, M, 4, and BRANCHED1 (BRC1) genes in the wild-type Columbia-0 accession and in different mutant backgrounds revealed that upregulation of strigolactone biosynthesis genes was triggered indirectly by the bacterial cytokinins via host-derived auxin and led to the activation of BRC1 expression, inhibiting the outgrowth of the newly developing shoots, a typical hallmark of leafy gall syndrome. Taken together, these data support the emerging insight that balances are critical for optimal leafy gall development: the long-lasting biotrophic interaction is possible only because the host activates a set of countermeasures including the strigolactone response in reaction to bacterial cytokinins to constrain the activity of R. fascians.},
  author       = {Stes, Elisabeth and Depuydt, Stephen and De Keyser, Annick and Matthys, Cedrick and Audenaert, Kris and Yoneyama, Koichi and Werbrouck, Stefaan and Goormachtig, Sofie and Vereecke, Danny},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {ANALOG GR24,RESPONSES,BUD OUTGROWTH,ACTS DOWNSTREAM,SIGNAL INTEGRATION,SYMPTOM DEVELOPMENT,MEDICAGO-TRUNCATULA,BOX PROTEIN MAX2,PLANT DEVELOPMENT,RHODOCOCCUS-FASCIANS INFECTION,witches' broom,Gram-positive phytopathogen,Apical dominance},
  language     = {eng},
  number       = {16},
  pages        = {5123--5134},
  title        = {Strigolactones as an auxiliary hormonal defence mechanism against leafy gall syndrome in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1093/jxb/erv309},
  volume       = {66},
  year         = {2015},
}

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