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In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography

Olivia Taylor, Nick Van Laeken (UGent) , Filip De Vos (UGent) , Ingeborgh Polis (UGent) , Tim Bosmans (UGent) , Ingeborg Goethals (UGent) , Eric Achten (UGent) , André Dobbeleir, Eva Vandermeulen (UGent) , Chris Baeken (UGent) , et al.
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Abstract
Background: [C-11]-3-amino-4-(2-dimethylaminomethyl-phenylsulfanyl)-benzonitrile ([C-11]DASB) is currently the mostly used radiotracer for positron emission tomography (PET) quantitative studies of the serotonin transporter (SERT) in the human brain but has never been validated in dogs. The first objective was therefore to evaluate normal [C-11]DASB distribution in different brain regions of healthy dogs using PET. The second objective was to provide less invasive and more convenient alternative methods to the arterial sampling-based kinetic analysis. Results: A dynamic acquisition of the brain was performed during 90 min. The PET images were coregistered with the magnetic resonance images taken prior to the study in order to manually drawn 20 regions of interest (ROIs). The highest radioactivity concentration of [C-11]DASB was observed in the hypothalamus, raphe nuclei and thalamus and lowest levels in the parietal cortex, occipital cortex and cerebellum. The regional radioactivity in those 20 ROIs was quantified using the multilinear reference tissue model 2 (MRTM2) and a semi-quantitative method. The values showed least variability between 40 and 60 min and this time interval was set as the optimal time interval for [C-11]DASB quantification in the canine brain. The correlation (R-2) between the MRTM2 and the semi-quantitative method using the data between 40 and 60 min was 99.3 % (two-tailed p-value < 0.01). Conclusions: The reference tissue models and semi-quantitative method provide a more convenient alternative to invasive arterial sampling models in the evaluation of the SERT of the normal canine brain. The optimal time interval for static scanning is set at 40 to 60 min after tracer injection.
Keywords
RADIOSYNTHESIS, RADIOLIGANDS, DOGS, [C-11]DASB, Dogs, PET, MODEL, I-123-5-I-R91150, GRAPHICAL ANALYSIS, Brain, OBSESSIVE-COMPULSIVE DISORDER, SEROTONIN 2A RECEPTOR, PET, Serotonin transporter, Multilinear reference tissue model 2, TRANSPORTER

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MLA
Taylor, Olivia, et al. “In Vivo Quantification of the [11C]DASB Binding in the Normal Canine Brain Using Positron Emission Tomography.” BMC VETERINARY RESEARCH, vol. 11, 2015, doi:10.1186/s12917-015-0622-3.
APA
Taylor, O., Van Laeken, N., De Vos, F., Polis, I., Bosmans, T., Goethals, I., … Peremans, K. (2015). In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography. BMC VETERINARY RESEARCH, 11. https://doi.org/10.1186/s12917-015-0622-3
Chicago author-date
Taylor, Olivia, Nick Van Laeken, Filip De Vos, Ingeborgh Polis, Tim Bosmans, Ingeborg Goethals, Eric Achten, et al. 2015. “In Vivo Quantification of the [11C]DASB Binding in the Normal Canine Brain Using Positron Emission Tomography.” BMC VETERINARY RESEARCH 11. https://doi.org/10.1186/s12917-015-0622-3.
Chicago author-date (all authors)
Taylor, Olivia, Nick Van Laeken, Filip De Vos, Ingeborgh Polis, Tim Bosmans, Ingeborg Goethals, Eric Achten, André Dobbeleir, Eva Vandermeulen, Chris Baeken, Jimmy Saunders, and Kathelijne Peremans. 2015. “In Vivo Quantification of the [11C]DASB Binding in the Normal Canine Brain Using Positron Emission Tomography.” BMC VETERINARY RESEARCH 11. doi:10.1186/s12917-015-0622-3.
Vancouver
1.
Taylor O, Van Laeken N, De Vos F, Polis I, Bosmans T, Goethals I, et al. In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography. BMC VETERINARY RESEARCH. 2015;11.
IEEE
[1]
O. Taylor et al., “In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography,” BMC VETERINARY RESEARCH, vol. 11, 2015.
@article{7076152,
  abstract     = {{Background: [C-11]-3-amino-4-(2-dimethylaminomethyl-phenylsulfanyl)-benzonitrile ([C-11]DASB) is currently the mostly used radiotracer for positron emission tomography (PET) quantitative studies of the serotonin transporter (SERT) in the human brain but has never been validated in dogs. The first objective was therefore to evaluate normal [C-11]DASB distribution in different brain regions of healthy dogs using PET. The second objective was to provide less invasive and more convenient alternative methods to the arterial sampling-based kinetic analysis. 
Results: A dynamic acquisition of the brain was performed during 90 min. The PET images were coregistered with the magnetic resonance images taken prior to the study in order to manually drawn 20 regions of interest (ROIs). The highest radioactivity concentration of [C-11]DASB was observed in the hypothalamus, raphe nuclei and thalamus and lowest levels in the parietal cortex, occipital cortex and cerebellum. 
The regional radioactivity in those 20 ROIs was quantified using the multilinear reference tissue model 2 (MRTM2) and a semi-quantitative method. The values showed least variability between 40 and 60 min and this time interval was set as the optimal time interval for [C-11]DASB quantification in the canine brain. The correlation (R-2) between the MRTM2 and the semi-quantitative method using the data between 40 and 60 min was 99.3 % (two-tailed p-value < 0.01). 
Conclusions: The reference tissue models and semi-quantitative method provide a more convenient alternative to invasive arterial sampling models in the evaluation of the SERT of the normal canine brain. The optimal time interval for static scanning is set at 40 to 60 min after tracer injection.}},
  articleno    = {{308}},
  author       = {{Taylor, Olivia and Van Laeken, Nick and De Vos, Filip and Polis, Ingeborgh and Bosmans, Tim and Goethals, Ingeborg and Achten, Eric and Dobbeleir, André and Vandermeulen, Eva and Baeken, Chris and Saunders, Jimmy and Peremans, Kathelijne}},
  issn         = {{1746-6148}},
  journal      = {{BMC VETERINARY RESEARCH}},
  keywords     = {{RADIOSYNTHESIS,RADIOLIGANDS,DOGS,[C-11]DASB,Dogs,PET,MODEL,I-123-5-I-R91150,GRAPHICAL ANALYSIS,Brain,OBSESSIVE-COMPULSIVE DISORDER,SEROTONIN 2A RECEPTOR,PET,Serotonin transporter,Multilinear reference tissue model 2,TRANSPORTER}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography}},
  url          = {{http://doi.org/10.1186/s12917-015-0622-3}},
  volume       = {{11}},
  year         = {{2015}},
}

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