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In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography

Olivia Taylor, Nick Van Laeken UGent, Filip De Vos UGent, Ingeborgh Polis UGent, Tim Bosmans UGent, Ingeborg Goethals UGent, Eric Achten UGent, André Dobbeleir, Eva Vandermeulen UGent, Chris Baeken UGent, et al. (2015) BMC VETERINARY RESEARCH. 11.
abstract
Background: [C-11]-3-amino-4-(2-dimethylaminomethyl-phenylsulfanyl)-benzonitrile ([C-11]DASB) is currently the mostly used radiotracer for positron emission tomography (PET) quantitative studies of the serotonin transporter (SERT) in the human brain but has never been validated in dogs. The first objective was therefore to evaluate normal [C-11]DASB distribution in different brain regions of healthy dogs using PET. The second objective was to provide less invasive and more convenient alternative methods to the arterial sampling-based kinetic analysis. Results: A dynamic acquisition of the brain was performed during 90 min. The PET images were coregistered with the magnetic resonance images taken prior to the study in order to manually drawn 20 regions of interest (ROIs). The highest radioactivity concentration of [C-11]DASB was observed in the hypothalamus, raphe nuclei and thalamus and lowest levels in the parietal cortex, occipital cortex and cerebellum. The regional radioactivity in those 20 ROIs was quantified using the multilinear reference tissue model 2 (MRTM2) and a semi-quantitative method. The values showed least variability between 40 and 60 min and this time interval was set as the optimal time interval for [C-11]DASB quantification in the canine brain. The correlation (R-2) between the MRTM2 and the semi-quantitative method using the data between 40 and 60 min was 99.3 % (two-tailed p-value < 0.01). Conclusions: The reference tissue models and semi-quantitative method provide a more convenient alternative to invasive arterial sampling models in the evaluation of the SERT of the normal canine brain. The optimal time interval for static scanning is set at 40 to 60 min after tracer injection.
Please use this url to cite or link to this publication:
author
organization
alternative title
In vivo quantification of the [C-11]DASB binding in the normal canine brain using positron emission tomography
year
type
journalArticle (original)
publication status
published
subject
keyword
RADIOSYNTHESIS, RADIOLIGANDS, DOGS, [C-11]DASB, Dogs, PET, MODEL, I-123-5-I-R91150, GRAPHICAL ANALYSIS, Brain, OBSESSIVE-COMPULSIVE DISORDER, SEROTONIN 2A RECEPTOR, PET, Serotonin transporter, Multilinear reference tissue model 2, TRANSPORTER
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
11
article number
308
pages
9 pages
Web of Science type
Article
Web of Science id
000367131600001
JCR category
VETERINARY SCIENCES
JCR impact factor
1.643 (2015)
JCR rank
27/138 (2015)
JCR quartile
1 (2015)
ISSN
1746-6148
DOI
10.1186/s12917-015-0622-3
project
The integrative neuroscience of behavioral control (Neuroscience)
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
7076152
handle
http://hdl.handle.net/1854/LU-7076152
date created
2016-02-08 13:47:10
date last changed
2017-06-08 11:43:04
@article{7076152,
  abstract     = {Background: [C-11]-3-amino-4-(2-dimethylaminomethyl-phenylsulfanyl)-benzonitrile ([C-11]DASB) is currently the mostly used radiotracer for positron emission tomography (PET) quantitative studies of the serotonin transporter (SERT) in the human brain but has never been validated in dogs. The first objective was therefore to evaluate normal [C-11]DASB distribution in different brain regions of healthy dogs using PET. The second objective was to provide less invasive and more convenient alternative methods to the arterial sampling-based kinetic analysis. 
Results: A dynamic acquisition of the brain was performed during 90 min. The PET images were coregistered with the magnetic resonance images taken prior to the study in order to manually drawn 20 regions of interest (ROIs). The highest radioactivity concentration of [C-11]DASB was observed in the hypothalamus, raphe nuclei and thalamus and lowest levels in the parietal cortex, occipital cortex and cerebellum. 
The regional radioactivity in those 20 ROIs was quantified using the multilinear reference tissue model 2 (MRTM2) and a semi-quantitative method. The values showed least variability between 40 and 60 min and this time interval was set as the optimal time interval for [C-11]DASB quantification in the canine brain. The correlation (R-2) between the MRTM2 and the semi-quantitative method using the data between 40 and 60 min was 99.3 \% (two-tailed p-value {\textlangle} 0.01). 
Conclusions: The reference tissue models and semi-quantitative method provide a more convenient alternative to invasive arterial sampling models in the evaluation of the SERT of the normal canine brain. The optimal time interval for static scanning is set at 40 to 60 min after tracer injection.},
  articleno    = {308},
  author       = {Taylor, Olivia and Van Laeken, Nick and De Vos, Filip and Polis, Ingeborgh and Bosmans, Tim and Goethals, Ingeborg and Achten, Eric and Dobbeleir, Andr{\'e} and Vandermeulen, Eva and Baeken, Chris and Saunders, Jimmy and Peremans, Kathelijne},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {RADIOSYNTHESIS,RADIOLIGANDS,DOGS,[C-11]DASB,Dogs,PET,MODEL,I-123-5-I-R91150,GRAPHICAL ANALYSIS,Brain,OBSESSIVE-COMPULSIVE DISORDER,SEROTONIN 2A RECEPTOR,PET,Serotonin transporter,Multilinear reference tissue model 2,TRANSPORTER},
  language     = {eng},
  pages        = {9},
  title        = {In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography},
  url          = {http://dx.doi.org/10.1186/s12917-015-0622-3},
  volume       = {11},
  year         = {2015},
}

Chicago
Taylor, Olivia, Nick Van Laeken, Filip De Vos, Ingeborgh Polis, Tim Bosmans, Ingeborg Goethals, Eric Achten, et al. 2015. “In Vivo Quantification of the [11C]DASB Binding in the Normal Canine Brain Using Positron Emission Tomography.” Bmc Veterinary Research 11.
APA
Taylor, O., Van Laeken, N., De Vos, F., Polis, I., Bosmans, T., Goethals, I., Achten, E., et al. (2015). In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography. BMC VETERINARY RESEARCH, 11.
Vancouver
1.
Taylor O, Van Laeken N, De Vos F, Polis I, Bosmans T, Goethals I, et al. In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography. BMC VETERINARY RESEARCH. 2015;11.
MLA
Taylor, Olivia, Nick Van Laeken, Filip De Vos, et al. “In Vivo Quantification of the [11C]DASB Binding in the Normal Canine Brain Using Positron Emission Tomography.” BMC VETERINARY RESEARCH 11 (2015): n. pag. Print.