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Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep

Antoine Poskin, Stephanie Verite, Loic Comtet, Yves Van der Stede, Brigitte Cay and Nick De Regge UGent (2015) VETERINARY RESEARCH. 46.
abstract
Schmallenberg virus (SBV) is an Orthobunyavirus that induces abortion, stillbirths and congenital malformations in ruminants. SBV infection induces a long lasting seroconversion under natural conditions. The persistence of the protective immunity and the isotype specific antibody response upon SBV infection of sheep has however not been studied in detail. Five sheep were kept in BSL3 facilities for more than 16 months and subjected to repeated SBV infections. Blood was regularly sampled and organs were collected at euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was determined with virus neutralization tests (VNT) and ELISAs. The primo SBV infection protected ewes against clinical signs, viraemia and virus replication in organs upon challenge infections more than 15 months later. Production of neutralizing SBV specific antibodies was first detected around 6 days post primo-inoculation with VNT and correlated with the appearance of SBV-N specific IgM antibodies. These IgM antibodies remained present for 2 weeks. SBV-N specific IgG antibodies were first detected between 10 and 21 dpi and reached a plateau at 28 dpi. This plateau remained consistently high and no significant decrease in titre was found over a period of more than 1 year. Similar results were found for the neutralising antibody response. In conclusion, the SBV specific IgM response probably eliminates SBV from the blood and the protective immunity induced by SBV infection protects sheep against reinfection for at least 16 months.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AKABANE VIRUS, REQUIRING NEUTRALIZING ANTIBODY, CATTLE, COMPLEMENT, SERUM, IMMUNIZATION, BLUETONGUE, CHALLENGE, RUMINANTS, GERMANY
journal title
VETERINARY RESEARCH
Vet. Res.
volume
46
article number
119
pages
9 pages
Web of Science type
Article
Web of Science id
000362879600001
JCR category
VETERINARY SCIENCES
JCR impact factor
2.928 (2015)
JCR rank
3/138 (2015)
JCR quartile
1 (2015)
ISSN
0928-4249
DOI
10.1186/s13567-015-0260-6
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
7071924
handle
http://hdl.handle.net/1854/LU-7071924
date created
2016-02-04 12:12:11
date last changed
2016-12-21 15:41:26
@article{7071924,
  abstract     = {Schmallenberg virus (SBV) is an Orthobunyavirus that induces abortion, stillbirths and congenital malformations in ruminants. SBV infection induces a long lasting seroconversion under natural conditions. The persistence of the protective immunity and the isotype specific antibody response upon SBV infection of sheep has however not been studied in detail. Five sheep were kept in BSL3 facilities for more than 16 months and subjected to repeated SBV infections. Blood was regularly sampled and organs were collected at euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was determined with virus neutralization tests (VNT) and ELISAs. The primo SBV infection protected ewes against clinical signs, viraemia and virus replication in organs upon challenge infections more than 15 months later. Production of neutralizing SBV specific antibodies was first detected around 6 days post primo-inoculation with VNT and correlated with the appearance of SBV-N specific IgM antibodies. These IgM antibodies remained present for 2 weeks. SBV-N specific IgG antibodies were first detected between 10 and 21 dpi and reached a plateau at 28 dpi. This plateau remained consistently high and no significant decrease in titre was found over a period of more than 1 year. Similar results were found for the neutralising antibody response. In conclusion, the SBV specific IgM response probably eliminates SBV from the blood and the protective immunity induced by SBV infection protects sheep against reinfection for at least 16 months.},
  articleno    = {119},
  author       = {Poskin, Antoine and Verite, Stephanie and Comtet, Loic and Van der Stede, Yves and Cay, Brigitte and De Regge, Nick},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {AKABANE VIRUS,REQUIRING NEUTRALIZING ANTIBODY,CATTLE,COMPLEMENT,SERUM,IMMUNIZATION,BLUETONGUE,CHALLENGE,RUMINANTS,GERMANY},
  language     = {eng},
  pages        = {9},
  title        = {Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep},
  url          = {http://dx.doi.org/10.1186/s13567-015-0260-6},
  volume       = {46},
  year         = {2015},
}

Chicago
Poskin, Antoine, Stephanie Verite, Loic Comtet, Yves Van der Stede, Brigitte Cay, and Nick De Regge. 2015. “Persistence of the Protective Immunity and Kinetics of the Isotype Specific Antibody Response Against the Viral Nucleocapsid Protein After Experimental Schmallenberg Virus Infection of Sheep.” Veterinary Research 46.
APA
Poskin, A., Verite, S., Comtet, L., Van der Stede, Y., Cay, B., & De Regge, N. (2015). Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep. VETERINARY RESEARCH, 46.
Vancouver
1.
Poskin A, Verite S, Comtet L, Van der Stede Y, Cay B, De Regge N. Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep. VETERINARY RESEARCH. 2015;46.
MLA
Poskin, Antoine, Stephanie Verite, Loic Comtet, et al. “Persistence of the Protective Immunity and Kinetics of the Isotype Specific Antibody Response Against the Viral Nucleocapsid Protein After Experimental Schmallenberg Virus Infection of Sheep.” VETERINARY RESEARCH 46 (2015): n. pag. Print.