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Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression

(2015) MOLECULAR BIOLOGY REPORTS. 42(8). p.1257-1264
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Abstract
Eukaryotic translation elongation factor 1 alpha (EEF1A) plays a key role in protein synthesis. In higher vertebrates EEF1A occurs in two isoforms, EEF1A1 and EEF1A2, encoded by distinct genes. The purpose of this study was to compare the two porcine genes as for the genomic sequence, gene organization and mRNA expression in different tissues, as well as to search for polymorphism and chromosomal assignment. Standard methods of DNA and mRNA analysis were used. We determined the complete genomic sequence of the porcine EEF1A1 and EEF1A2 genes. The two genes differ in the lengths of transcription units (3102 and 8588 bp, respectively), but have similar genomic organization and their coding sequences are highly similar (78 % identity of coding sequences and 92.4 % identity of amino acid sequences). Several polymorphisms in the two genes were detected. EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively. mRNA of EEF1A1 was expressed in all studied tissues (the highest expression was in 44-day fetal muscle and low expression in adult liver and brain), while EEF1A2 was expressed only in skeletal-muscle, tongue, heart, diaphragm and brain tissues. EEF1A2 was not expressed in fetal muscle tissue (44 days). In this paper results are provided on genomic sequences, genomic organization, polymorphism, chromosomal assignment and spatial and temporal expressions of the porcine EEF1A1 and EEF1A2 genes. Novel polymorphisms were described in both genes. Porcine EEF1A2 was studied for the first time.
Keywords
TISSUE, S1, HIGH-RESOLUTION, ELONGATION FACTOR-1-ALPHA, Pig, Gene organization, Gene mapping, ISOFORM, PROTEIN, PIG, MUSCLE, SEQUENCE, LINKAGE, Gene expression, EEF1A2, EEF1A1

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MLA
Svobodová, Kateřina et al. “Porcine EEF1A1 and EEF1A2 Genes: Genomic Structure, Polymorphism, Mapping and Expression.” MOLECULAR BIOLOGY REPORTS 42.8 (2015): 1257–1264. Print.
APA
Svobodová, K., Horák, P., Stratil, A., Bartenschlager, H., Van Poucke, M., Chalupová, P., Dvořáková, V., et al. (2015). Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression. MOLECULAR BIOLOGY REPORTS, 42(8), 1257–1264.
Chicago author-date
Svobodová, Kateřina, Pavel Horák, Antonín Stratil, Heinz Bartenschlager, Mario Van Poucke, Pavla Chalupová, Věra Dvořáková, et al. 2015. “Porcine EEF1A1 and EEF1A2 Genes: Genomic Structure, Polymorphism, Mapping and Expression.” Molecular Biology Reports 42 (8): 1257–1264.
Chicago author-date (all authors)
Svobodová, Kateřina, Pavel Horák, Antonín Stratil, Heinz Bartenschlager, Mario Van Poucke, Pavla Chalupová, Věra Dvořáková, Christoph Knorr, Roman Stupka, Jaroslav Čítek, Michal Šprysl, Anna Palánová, Luc Peelman, Hermann Geldermann, and Aleš Knoll. 2015. “Porcine EEF1A1 and EEF1A2 Genes: Genomic Structure, Polymorphism, Mapping and Expression.” Molecular Biology Reports 42 (8): 1257–1264.
Vancouver
1.
Svobodová K, Horák P, Stratil A, Bartenschlager H, Van Poucke M, Chalupová P, et al. Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression. MOLECULAR BIOLOGY REPORTS. 2015;42(8):1257–64.
IEEE
[1]
K. Svobodová et al., “Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression,” MOLECULAR BIOLOGY REPORTS, vol. 42, no. 8, pp. 1257–1264, 2015.
@article{7066453,
  abstract     = {Eukaryotic translation elongation factor 1 alpha (EEF1A) plays a key role in protein synthesis. In higher vertebrates EEF1A occurs in two isoforms, EEF1A1 and EEF1A2, encoded by distinct genes. The purpose of this study was to compare the two porcine genes as for the genomic sequence, gene organization and mRNA expression in different tissues, as well as to search for polymorphism and chromosomal assignment. Standard methods of DNA and mRNA analysis were used. We determined the complete genomic sequence of the porcine EEF1A1 and EEF1A2 genes. The two genes differ in the lengths of transcription units (3102 and 8588 bp, respectively), but have similar genomic organization and their coding sequences are highly similar (78 % identity of coding sequences and 92.4 % identity of amino acid sequences). Several polymorphisms in the two genes were detected. EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively. mRNA of EEF1A1 was expressed in all studied tissues (the highest expression was in 44-day fetal muscle and low expression in adult liver and brain), while EEF1A2 was expressed only in skeletal-muscle, tongue, heart, diaphragm and brain tissues. EEF1A2 was not expressed in fetal muscle tissue (44 days). In this paper results are provided on genomic sequences, genomic organization, polymorphism, chromosomal assignment and spatial and temporal expressions of the porcine EEF1A1 and EEF1A2 genes. Novel polymorphisms were described in both genes. Porcine EEF1A2 was studied for the first time.},
  author       = {Svobodová, Kateřina and Horák, Pavel and Stratil, Antonín and Bartenschlager, Heinz and Van Poucke, Mario and Chalupová, Pavla and Dvořáková, Věra and Knorr, Christoph and Stupka, Roman and Čítek, Jaroslav and Šprysl, Michal and Palánová, Anna and Peelman, Luc and Geldermann, Hermann and Knoll, Aleš},
  issn         = {0301-4851},
  journal      = {MOLECULAR BIOLOGY REPORTS},
  keywords     = {TISSUE,S1,HIGH-RESOLUTION,ELONGATION FACTOR-1-ALPHA,Pig,Gene organization,Gene mapping,ISOFORM,PROTEIN,PIG,MUSCLE,SEQUENCE,LINKAGE,Gene expression,EEF1A2,EEF1A1},
  language     = {eng},
  number       = {8},
  pages        = {1257--1264},
  title        = {Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression},
  url          = {http://dx.doi.org/10.1007/s11033-015-3866-x},
  volume       = {42},
  year         = {2015},
}

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