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Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection

Valentina Goftman (UGent) , Tangi Aubert (UGent) , Dries Vande Ginste (UGent) , Rik Van Deun (UGent) , Natalia Beloglazova (UGent) , Zeger Hens (UGent) , Sarah De Saeger (UGent) and Irina Goryacheva
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Abstract
To create bright and stable fluorescent biolabels for immunoassay detection of mycotoxin deoxynivalenol in food and feed, CdSe/CdS/ZnS core-shell quantum dots (QDs) were encapsulated in silica nanoparticles through a water-in-oil reverse microemulsion process. The optical properties and stability of the obtained silica coated QDs (QD@SiO2), modified with amino, carboxyl and epoxy groups and stabilized with polyethylene glycol fragments, were characterized in order to assess their bioapplicability. The developed co-condensation techniques allowed maintaining 80% of the initial fluorescent properties and yielded stable fluorescent labels that could be easily activated and bioconjugated. Further, the modified QD@SiO2 were efficiently conjugated with antibodies and applied as a novel label in a microtiter plate based immunoassay and a quantitative column-based rapid immunotest for deoxynivalenol detection with IC50 of 473 and 20 ng/ml, respectively.
Keywords
FLISA, Immunoassay, PEGylation, Surface functionalization, CELLS, Nanocrystals, SPHERES, NANOPARTICLES, Microemulsion, MICROEMULSION METHOD, IBCN

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Citation

Please use this url to cite or link to this publication:

MLA
Goftman, Valentina et al. “Synthesis, Modification, Bioconjugation of Silica Coated Fluorescent Quantum Dots and Their Application for Mycotoxin Detection.” BIOSENSORS & BIOELECTRONICS 79 (2016): 476–481. Print.
APA
Goftman, V., Aubert, T., Vande Ginste, D., Van Deun, R., Beloglazova, N., Hens, Z., De Saeger, S., et al. (2016). Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection. BIOSENSORS & BIOELECTRONICS, 79, 476–481.
Chicago author-date
Goftman, Valentina, Tangi Aubert, Dries Vande Ginste, Rik Van Deun, Natalia Beloglazova, Zeger Hens, Sarah De Saeger, and Irina Goryacheva. 2016. “Synthesis, Modification, Bioconjugation of Silica Coated Fluorescent Quantum Dots and Their Application for Mycotoxin Detection.” Biosensors & Bioelectronics 79: 476–481.
Chicago author-date (all authors)
Goftman, Valentina, Tangi Aubert, Dries Vande Ginste, Rik Van Deun, Natalia Beloglazova, Zeger Hens, Sarah De Saeger, and Irina Goryacheva. 2016. “Synthesis, Modification, Bioconjugation of Silica Coated Fluorescent Quantum Dots and Their Application for Mycotoxin Detection.” Biosensors & Bioelectronics 79: 476–481.
Vancouver
1.
Goftman V, Aubert T, Vande Ginste D, Van Deun R, Beloglazova N, Hens Z, et al. Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection. BIOSENSORS & BIOELECTRONICS. 2016;79:476–81.
IEEE
[1]
V. Goftman et al., “Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection,” BIOSENSORS & BIOELECTRONICS, vol. 79, pp. 476–481, 2016.
@article{7066231,
  abstract     = {To create bright and stable fluorescent biolabels for immunoassay detection of mycotoxin deoxynivalenol in food and feed, CdSe/CdS/ZnS core-shell quantum dots (QDs) were encapsulated in silica nanoparticles through a water-in-oil reverse microemulsion process. The optical properties and stability of the obtained silica coated QDs (QD@SiO2), modified with amino, carboxyl and epoxy groups and stabilized with polyethylene glycol fragments, were characterized in order to assess their bioapplicability. The developed co-condensation techniques allowed maintaining 80% of the initial fluorescent properties and yielded stable fluorescent labels that could be easily activated and bioconjugated. Further, the modified QD@SiO2 were efficiently conjugated with antibodies and applied as a novel label in a microtiter plate based immunoassay and a quantitative column-based rapid immunotest for deoxynivalenol detection with IC50 of 473 and 20 ng/ml, respectively.},
  author       = {Goftman, Valentina and Aubert, Tangi and Vande Ginste, Dries and Van Deun, Rik and Beloglazova, Natalia and Hens, Zeger and De Saeger, Sarah and Goryacheva, Irina},
  issn         = {0956-5663},
  journal      = {BIOSENSORS & BIOELECTRONICS},
  keywords     = {FLISA,Immunoassay,PEGylation,Surface functionalization,CELLS,Nanocrystals,SPHERES,NANOPARTICLES,Microemulsion,MICROEMULSION METHOD,IBCN},
  language     = {eng},
  pages        = {476--481},
  title        = {Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection},
  url          = {http://dx.doi.org/10.1016/j.bios.2015.12.079},
  volume       = {79},
  year         = {2016},
}

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