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Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2

Yuanyuan Mei, Peter Thorpe, Athanas Guzha, Annelies Haegeman, Vivian C Blok, Katrin MacKenzie, Godelieve Gheysen UGent, John Jones UGent and Sophie Mantelin (2015) NEMATOLOGY. 17(4). p.409-424
abstract
Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Gpa2 gene, dorsal pharyngeal gland, hypersensitive response, parasitism, plant cell death, SPRYSEC protein, PLANT-PARASITIC NEMATODE, NB-LRR PROTEIN, CYST-NEMATODE, DISEASE RESISTANCE, FUNCTIONAL-CHARACTERIZATION, HETERODERA-SCHACHTII, ROSTOCHIENSIS, SEQUENCE, SYSTEM, GENOME
journal title
NEMATOLOGY
Nematology
volume
17
issue
4
pages
409 - 424
Web of Science type
Article
Web of Science id
000353393400003
JCR category
ZOOLOGY
JCR impact factor
1.061 (2015)
JCR rank
74/160 (2015)
JCR quartile
2 (2015)
ISSN
1388-5545
DOI
10.1163/15685411-00002875
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7065802
handle
http://hdl.handle.net/1854/LU-7065802
date created
2016-02-01 20:17:52
date last changed
2016-12-19 15:39:21
@article{7065802,
  abstract     = {Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10\%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana.},
  author       = {Mei, Yuanyuan and Thorpe, Peter and Guzha, Athanas and Haegeman, Annelies and Blok, Vivian C and MacKenzie, Katrin and Gheysen, Godelieve and Jones, John and Mantelin, Sophie},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {Gpa2 gene,dorsal pharyngeal gland,hypersensitive response,parasitism,plant cell death,SPRYSEC protein,PLANT-PARASITIC NEMATODE,NB-LRR PROTEIN,CYST-NEMATODE,DISEASE RESISTANCE,FUNCTIONAL-CHARACTERIZATION,HETERODERA-SCHACHTII,ROSTOCHIENSIS,SEQUENCE,SYSTEM,GENOME},
  language     = {eng},
  number       = {4},
  pages        = {409--424},
  title        = {Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2},
  url          = {http://dx.doi.org/10.1163/15685411-00002875},
  volume       = {17},
  year         = {2015},
}

Chicago
Mei, Yuanyuan, Peter Thorpe, Athanas Guzha, Annelies Haegeman, Vivian C Blok, Katrin MacKenzie, Godelieve Gheysen, John Jones, and Sophie Mantelin. 2015. “Only a Small Subset of the SPRY Domain Gene Family in Globodera Pallida Is Likely to Encode Effectors, Two of Which Suppress Host Defences Induced by the Potato Resistance Gene Gpa2.” Nematology 17 (4): 409–424.
APA
Mei, Y., Thorpe, P., Guzha, A., Haegeman, A., Blok, V. C., MacKenzie, K., Gheysen, G., et al. (2015). Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2. NEMATOLOGY, 17(4), 409–424.
Vancouver
1.
Mei Y, Thorpe P, Guzha A, Haegeman A, Blok VC, MacKenzie K, et al. Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2. NEMATOLOGY. 2015;17(4):409–24.
MLA
Mei, Yuanyuan, Peter Thorpe, Athanas Guzha, et al. “Only a Small Subset of the SPRY Domain Gene Family in Globodera Pallida Is Likely to Encode Effectors, Two of Which Suppress Host Defences Induced by the Potato Resistance Gene Gpa2.” NEMATOLOGY 17.4 (2015): 409–424. Print.