Functional analysis of an auxin-inducible DNA-binding protein gene
- Author
- Any Bernstein, Amanda Mangeon, Janice Almeida-Engler, Gilbert Engler, Marc Van Montagu (UGent) , Gilberto Sachetto-Martins and Dulce De Oliveira (UGent)
- Organization
- Abstract
- Over the past decades, several studies indicate a correlation between the phytohormone auxin and cell division. The molecular players of this signaling pathway are now being uncovered. DNA Binding Protein1 from Arabidopsis (AtDBP1) is an auxin-inducible gene able to bind DNA non-specifically. In this work the tissue-expression pattern of this gene was investigated. Promoter-GUS analysis demonstrated that the AtDBP1 promoter is active in regions exhibiting intense cell division such as meristems and nematode feeding sites. Also, the promoter expression was modulated upon incubation with cell cycle blockers, indicating a potential role in cell division for this gene. Lastly, AtDBP1 antisense plants presented a higher insensitivity to auxin, and interfered negatively with auxin-induced callus formation and reduced apical dominance.
- Keywords
- auxin, cell cycle, DNA-binding protein1 (DBP1), AtDBP1, Arabidopsis thaliana, developmental gene expression, nematode
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-7065437
- MLA
- Bernstein, Any, et al. “Functional Analysis of an Auxin-Inducible DNA-Binding Protein Gene.” PLANT SIGNALING & BEHAVIOR, vol. 10, no. 1, 2015, doi:10.4161/15592324.2014.977706.
- APA
- Bernstein, A., Mangeon, A., Almeida-Engler, J., Engler, G., Van Montagu, M., Sachetto-Martins, G., & De Oliveira, D. (2015). Functional analysis of an auxin-inducible DNA-binding protein gene. PLANT SIGNALING & BEHAVIOR, 10(1). https://doi.org/10.4161/15592324.2014.977706
- Chicago author-date
- Bernstein, Any, Amanda Mangeon, Janice Almeida-Engler, Gilbert Engler, Marc Van Montagu, Gilberto Sachetto-Martins, and Dulce De Oliveira. 2015. “Functional Analysis of an Auxin-Inducible DNA-Binding Protein Gene.” PLANT SIGNALING & BEHAVIOR 10 (1). https://doi.org/10.4161/15592324.2014.977706.
- Chicago author-date (all authors)
- Bernstein, Any, Amanda Mangeon, Janice Almeida-Engler, Gilbert Engler, Marc Van Montagu, Gilberto Sachetto-Martins, and Dulce De Oliveira. 2015. “Functional Analysis of an Auxin-Inducible DNA-Binding Protein Gene.” PLANT SIGNALING & BEHAVIOR 10 (1). doi:10.4161/15592324.2014.977706.
- Vancouver
- 1.Bernstein A, Mangeon A, Almeida-Engler J, Engler G, Van Montagu M, Sachetto-Martins G, et al. Functional analysis of an auxin-inducible DNA-binding protein gene. PLANT SIGNALING & BEHAVIOR. 2015;10(1).
- IEEE
- [1]A. Bernstein et al., “Functional analysis of an auxin-inducible DNA-binding protein gene,” PLANT SIGNALING & BEHAVIOR, vol. 10, no. 1, 2015.
@article{7065437,
abstract = {{Over the past decades, several studies indicate a correlation between the phytohormone auxin and cell division. The molecular players of this signaling pathway are now being uncovered. DNA Binding Protein1 from Arabidopsis (AtDBP1) is an auxin-inducible gene able to bind DNA non-specifically. In this work the tissue-expression pattern of this gene was investigated. Promoter-GUS analysis demonstrated that the AtDBP1 promoter is active in regions exhibiting intense cell division such as meristems and nematode feeding sites. Also, the promoter expression was modulated upon incubation with cell cycle blockers, indicating a potential role in cell division for this gene. Lastly, AtDBP1 antisense plants presented a higher insensitivity to auxin, and interfered negatively with auxin-induced callus formation and reduced apical dominance.}},
articleno = {{e977706}},
author = {{Bernstein, Any and Mangeon, Amanda and Almeida-Engler, Janice and Engler, Gilbert and Van Montagu, Marc and Sachetto-Martins, Gilberto and De Oliveira, Dulce}},
issn = {{1559-2316}},
journal = {{PLANT SIGNALING & BEHAVIOR}},
keywords = {{auxin,cell cycle,DNA-binding protein1 (DBP1),AtDBP1,Arabidopsis thaliana,developmental gene expression,nematode}},
language = {{eng}},
number = {{1}},
pages = {{5}},
title = {{Functional analysis of an auxin-inducible DNA-binding protein gene}},
url = {{http://dx.doi.org/10.4161/15592324.2014.977706}},
volume = {{10}},
year = {{2015}},
}
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