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The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells

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Center for nano- and biophotonics (NB-Photonics)
Abstract
Background: Recent reports highlighting the role of particle geometry have suggested that anisotropy can affect the rate and the pathway of particle uptake by cells. Therefore, we investigate the internalization by cells of porous calcium carbonate particles with different shapes and anisotropies. Results: We report here on a new method of the synthesis of polyelectrolyte coated calcium carbonate particles whose geometry was controlled by varying the mixing speed and time, pH value of the reaction solution, and ratio of the interacting salts used for particle formation. Uptake of spherical, cuboidal, ellipsoidal (with two different sizes) polyelectrolyte coated calcium carbonate particles was studied in cervical carcinoma cells. Quantitative data were obtained from the analysis of confocal laser scanning microscopy images. Conclusions: Our results indicate that the number of internalized calcium carbonate particles depends on the aspect ratio of the particle, whereby elongated particles (higher aspect ratio) are internalized with a higher frequency than more spherical particles (lower aspect ratio). The total volume of internalized particles scales with the volume of the individual particles, in case equal amount of particles were added per cell.
Keywords
CALCIUM-CARBONATE MICROPARTICLES, POLYELECTROLYTE MULTILAYER CAPSULES, MESENCHYMAL STEM-CELLS, DRUG-DELIVERY, CELLULAR UPTAKE, GOLD NANOPARTICLES, MAMMALIAN-CELLS, CANCER-CELLS, INTERNALIZATION PATHWAYS, SILVER NANOPARTICLES, Internalisation, Cells, Uptake, Anisotropic, Calcium carbonate

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Citation

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Chicago
Parakhonskiy, Bogdan, Mikhail V Zyuzin, Alexey Yashchenok, Susana Carregal-Romero, Joanna Rejman, Helmuth Möhwald, Wolfgang J Parak, and Andre Skirtach. 2015. “The Influence of the Size and Aspect Ratio of Anisotropic, Porous CaCO3 Particles on Their Uptake by Cells.” Journal of Nanobiotechnology 13.
APA
Parakhonskiy, B., Zyuzin, M. V., Yashchenok, A., Carregal-Romero, S., Rejman, J., Möhwald, H., Parak, W. J., et al. (2015). The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells. JOURNAL OF NANOBIOTECHNOLOGY, 13.
Vancouver
1.
Parakhonskiy B, Zyuzin MV, Yashchenok A, Carregal-Romero S, Rejman J, Möhwald H, et al. The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells. JOURNAL OF NANOBIOTECHNOLOGY. 2015;13.
MLA
Parakhonskiy, Bogdan, Mikhail V Zyuzin, Alexey Yashchenok, et al. “The Influence of the Size and Aspect Ratio of Anisotropic, Porous CaCO3 Particles on Their Uptake by Cells.” JOURNAL OF NANOBIOTECHNOLOGY 13 (2015): n. pag. Print.
@article{7064083,
  abstract     = {Background: Recent reports highlighting the role of particle geometry have suggested that anisotropy can affect the rate and the pathway of particle uptake by cells. Therefore, we investigate the internalization by cells of porous calcium carbonate particles with different shapes and anisotropies. 
Results: We report here on a new method of the synthesis of polyelectrolyte coated calcium carbonate particles whose geometry was controlled by varying the mixing speed and time, pH value of the reaction solution, and ratio of the interacting salts used for particle formation. Uptake of spherical, cuboidal, ellipsoidal (with two different sizes) polyelectrolyte coated calcium carbonate particles was studied in cervical carcinoma cells. Quantitative data were obtained from the analysis of confocal laser scanning microscopy images. 
Conclusions: Our results indicate that the number of internalized calcium carbonate particles depends on the aspect ratio of the particle, whereby elongated particles (higher aspect ratio) are internalized with a higher frequency than more spherical particles (lower aspect ratio). The total volume of internalized particles scales with the volume of the individual particles, in case equal amount of particles were added per cell.},
  articleno    = {53},
  author       = {Parakhonskiy, Bogdan and Zyuzin, Mikhail V and Yashchenok, Alexey and Carregal-Romero, Susana and Rejman, Joanna and M{\"o}hwald, Helmuth and Parak, Wolfgang J and Skirtach, Andre},
  issn         = {1477-3155},
  journal      = {JOURNAL OF NANOBIOTECHNOLOGY},
  keyword      = {CALCIUM-CARBONATE MICROPARTICLES,POLYELECTROLYTE MULTILAYER CAPSULES,MESENCHYMAL STEM-CELLS,DRUG-DELIVERY,CELLULAR UPTAKE,GOLD NANOPARTICLES,MAMMALIAN-CELLS,CANCER-CELLS,INTERNALIZATION PATHWAYS,SILVER NANOPARTICLES,Internalisation,Cells,Uptake,Anisotropic,Calcium carbonate},
  language     = {eng},
  pages        = {13},
  title        = {The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells},
  url          = {http://dx.doi.org/10.1186/s12951-015-0111-7},
  volume       = {13},
  year         = {2015},
}

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