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Analysis of cbbL, nifH, and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria

Guillaume Tahon (UGent) , Bjorn Tytgat (UGent) , Pieter Stragier (UGent) and Anne Willems (UGent)
(2016) MICROBIAL ECOLOGY. 71(1). p.131-149
Author
Organization
Project
CCAMBIO (BelSPO SD/BA/03A)
Project
FWO G014612
Abstract
Cyanobacteria are generally thought to be responsible for primary production and nitrogen fixation in the microbial communities that dominate Antarctic ecosystems. Recent studies of bacterial communities in terrestrial Antarctica, however, have shown that Cyanobacteria are sometimes only scarcely present, suggesting that other bacteria presumably take over their role as primary producers and diazotrophs. The diversity of key genes in these processes was studied in surface samples from the Sor Rondane Mountains, Dronning Maud Land, using clone libraries of the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL, cbbM) and dinitrogenase-reductase (nifH) genes. We recovered a large diversity of non-cyanobacterial cbbL type IC in addition to cyanobacterial type IB, suggesting that non-cyanobacterial autotrophs may contribute to primary production. The nifH diversity recovered was predominantly related to Cyanobacteria, particularly members of the Nostocales. We also investigated the occurrence of proteorhodopsin and anoxygenic phototrophy as mechanisms for non-Cyanobacteria to exploit solar energy. While proteorhodopsin genes were not detected, a large diversity of genes coding for the light and medium subunits of the type 2 phototrophic reaction center (pufLM) was observed, suggesting for the first time, that the aerobic photoheterotrophic lifestyle may be important in oligotrophic high-altitude ice-free terrestrial Antarctic habitats.
Keywords
Clone libraries, RuBisCO, Antarctica, nifH, Bacteria, pufLM, AEROBIC ANOXYGENIC PHOTOTROPHS, RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE GENES, RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE, LARGE-SUBUNIT GENES, COMMUNITY STRUCTURE, FRESH-WATER, HORIZONTAL TRANSFER, MEDITERRANEAN SEA, INTERACTIVE TREE, TIBETAN PLATEAU

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Citation

Please use this url to cite or link to this publication:

Chicago
Tahon, Guillaume, Bjorn Tytgat, Pieter Stragier, and Anne Willems. 2016. “Analysis of cbbL, nifH, and pufLM in Soils from the Sør Rondane Mountains, Antarctica, Reveals a Large Diversity of Autotrophic and Phototrophic Bacteria.” Microbial Ecology 71 (1): 131–149.
APA
Tahon, G., Tytgat, B., Stragier, P., & Willems, A. (2016). Analysis of cbbL, nifH, and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria. MICROBIAL ECOLOGY, 71(1), 131–149.
Vancouver
1.
Tahon G, Tytgat B, Stragier P, Willems A. Analysis of cbbL, nifH, and pufLM in soils from the Sør Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria. MICROBIAL ECOLOGY. 2016;71(1):131–49.
MLA
Tahon, Guillaume, Bjorn Tytgat, Pieter Stragier, et al. “Analysis of cbbL, nifH, and pufLM in Soils from the Sør Rondane Mountains, Antarctica, Reveals a Large Diversity of Autotrophic and Phototrophic Bacteria.” MICROBIAL ECOLOGY 71.1 (2016): 131–149. Print.
@article{7061120,
  abstract     = {Cyanobacteria are generally thought to be responsible for primary production and nitrogen fixation in the microbial communities that dominate Antarctic ecosystems. Recent studies of bacterial communities in terrestrial Antarctica, however, have shown that Cyanobacteria are sometimes only scarcely present, suggesting that other bacteria presumably take over their role as primary producers and diazotrophs. The diversity of key genes in these processes was studied in surface samples from the Sor Rondane Mountains, Dronning Maud Land, using clone libraries of the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL, cbbM) and dinitrogenase-reductase (nifH) genes. We recovered a large diversity of non-cyanobacterial cbbL type IC in addition to cyanobacterial type IB, suggesting that non-cyanobacterial autotrophs may contribute to primary production. The nifH diversity recovered was predominantly related to Cyanobacteria, particularly members of the Nostocales. We also investigated the occurrence of proteorhodopsin and anoxygenic phototrophy as mechanisms for non-Cyanobacteria to exploit solar energy. While proteorhodopsin genes were not detected, a large diversity of genes coding for the light and medium subunits of the type 2 phototrophic reaction center (pufLM) was observed, suggesting for the first time, that the aerobic photoheterotrophic lifestyle may be important in oligotrophic high-altitude ice-free terrestrial Antarctic habitats.},
  author       = {Tahon, Guillaume and Tytgat, Bjorn and Stragier, Pieter and Willems, Anne},
  issn         = {0095-3628},
  journal      = {MICROBIAL ECOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {131--149},
  title        = {Analysis of cbbL, nifH, and pufLM in soils from the S{\o}r Rondane Mountains, Antarctica, reveals a large diversity of autotrophic and phototrophic bacteria},
  url          = {http://dx.doi.org/10.1007/s00248-015-0704-6},
  volume       = {71},
  year         = {2016},
}

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