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Biofilm formation of ica operon-positive Staphylococcus epidermidis from different sources

Maria Angeles Argudín, Wannes Vanderhaeghen, Stien Vandendriessche, Ilse Vandecandelaere, Olivier Denis, Tom Coenye UGent and Patrick Butaye UGent (2015) APMIS. 123(12). p.1081-1089
abstract
Information on the prevalence of biofilm-related factors (PIA, Bhp, Aap, Embp) in Staphylococcus epidermidis of animal origin is scarce. In this study, 263 S.epidermidis isolates of diverse origin (animal, farmers, patients, and laboratory staff) were investigated for the presence of the ica operon (icaRADBC). The icaRADBC-positive isolates were further characterized by means of biofilm formation, presence of other biofilm-related genes, antimicrobial resistance, and population structure. Of all isolates, 28.5% (n=75) were icaRADBC-positive, including 16.5% of animal origin, 29.1% farmer isolates, and 44.6% hospital-associated isolates (including patients and laboratory staff isolates). Most icaRADBC-positive isolates carried embp (n=73), aap (n=57), bhp (n=22), and IS256 (n=29). Statistical differences were found between animal and patient isolates for the presence of icaRADBC, bhp, and aap. No statistically significant relation was found between the presence of one or more genes and the level of biofilm formation. Most icaRADBC-positive isolates belonged to the clonal complex 5 (formerly 2) and most sequence types corresponded to types previously observed in community and nosocomial S.epidermidis populations. Although the prevalence of S.epidermidis in the nasal cavity of bovines and poultry is low, some isolates belong to STs related to ica-positive clinical strains.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IS256, mecA, Gentamicin, multi-locus sequence typing, polysaccharide intercellular adhesin, livestock, COAGULASE-NEGATIVE STAPHYLOCOCCI, ANTIMICROBIAL RESISTANCE, POPULATION-STRUCTURE, STRAINS, INFECTIONS, DIVERSITY, GENES, FARMS, EPIDEMIOLOGY, DISCRIMINATE
journal title
APMIS
APMIS
volume
123
issue
12
pages
1081 - 1089
Web of Science type
Article
Web of Science id
000364779600011
JCR category
PATHOLOGY
JCR impact factor
1.933 (2015)
JCR rank
37/78 (2015)
JCR quartile
2 (2015)
ISSN
0903-4641
DOI
10.1111/apm.12472
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7058410
handle
http://hdl.handle.net/1854/LU-7058410
date created
2016-01-28 09:39:14
date last changed
2016-12-19 15:39:11
@article{7058410,
  abstract     = {Information on the prevalence of biofilm-related factors (PIA, Bhp, Aap, Embp) in Staphylococcus epidermidis of animal origin is scarce. In this study, 263 S.epidermidis isolates of diverse origin (animal, farmers, patients, and laboratory staff) were investigated for the presence of the ica operon (icaRADBC). The icaRADBC-positive isolates were further characterized by means of biofilm formation, presence of other biofilm-related genes, antimicrobial resistance, and population structure. Of all isolates, 28.5\% (n=75) were icaRADBC-positive, including 16.5\% of animal origin, 29.1\% farmer isolates, and 44.6\% hospital-associated isolates (including patients and laboratory staff isolates). Most icaRADBC-positive isolates carried embp (n=73), aap (n=57), bhp (n=22), and IS256 (n=29). Statistical differences were found between animal and patient isolates for the presence of icaRADBC, bhp, and aap. No statistically significant relation was found between the presence of one or more genes and the level of biofilm formation. Most icaRADBC-positive isolates belonged to the clonal complex 5 (formerly 2) and most sequence types corresponded to types previously observed in community and nosocomial S.epidermidis populations. Although the prevalence of S.epidermidis in the nasal cavity of bovines and poultry is low, some isolates belong to STs related to ica-positive clinical strains.},
  author       = {Argud{\'i}n, Maria Angeles and Vanderhaeghen, Wannes and Vandendriessche, Stien and Vandecandelaere, Ilse and Denis, Olivier and Coenye, Tom and Butaye, Patrick},
  issn         = {0903-4641},
  journal      = {APMIS},
  keyword      = {IS256,mecA,Gentamicin,multi-locus sequence typing,polysaccharide intercellular adhesin,livestock,COAGULASE-NEGATIVE STAPHYLOCOCCI,ANTIMICROBIAL RESISTANCE,POPULATION-STRUCTURE,STRAINS,INFECTIONS,DIVERSITY,GENES,FARMS,EPIDEMIOLOGY,DISCRIMINATE},
  language     = {eng},
  number       = {12},
  pages        = {1081--1089},
  title        = {Biofilm formation of ica operon-positive Staphylococcus epidermidis from different sources},
  url          = {http://dx.doi.org/10.1111/apm.12472},
  volume       = {123},
  year         = {2015},
}

Chicago
Argudín, Maria Angeles, Wannes Vanderhaeghen, Stien Vandendriessche, Ilse Vandecandelaere, Olivier Denis, Tom Coenye, and Patrick Butaye. 2015. “Biofilm Formation of Ica Operon-positive Staphylococcus Epidermidis from Different Sources.” Apmis 123 (12): 1081–1089.
APA
Argudín, Maria Angeles, Vanderhaeghen, W., Vandendriessche, S., Vandecandelaere, I., Denis, O., Coenye, T., & Butaye, P. (2015). Biofilm formation of ica operon-positive Staphylococcus epidermidis from different sources. APMIS, 123(12), 1081–1089.
Vancouver
1.
Argudín MA, Vanderhaeghen W, Vandendriessche S, Vandecandelaere I, Denis O, Coenye T, et al. Biofilm formation of ica operon-positive Staphylococcus epidermidis from different sources. APMIS. 2015;123(12):1081–9.
MLA
Argudín, Maria Angeles, Wannes Vanderhaeghen, Stien Vandendriessche, et al. “Biofilm Formation of Ica Operon-positive Staphylococcus Epidermidis from Different Sources.” APMIS 123.12 (2015): 1081–1089. Print.