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The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation

(2016) JOURNAL OF GENERAL VIROLOGY. 97(3). p.778-785
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Abstract
HIV-1-mediated CD4 down-regulation is an important determinant of viral replication in vivo. Research on cellular co-factors involved in this process could lead to the identification of potential therapeutic targets. We found that CD4 surface levels were significantly higher in HIV-1-infected cells knocked-down for the HIV Rev-Binding protein (HRB) compared to control cells. HRB knock-down affected CD4 down-regulation induced by Nef but not by HIV-1 Vpu. Interestingly, the knock-down of the related protein HRBL (HRB Like), but not of the HRB interaction partner EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15), increased CD4 levels in Vpu-expressing cells significantly. Both these proteins are known to be involved in HIV-1-mediated CD4 down-regulation as cofactors of HIV-1 Nef. These results identify HRB as a previously unknown co-factor for HIV-1 Nef-mediated CD4 down-regulation and highlight differences with the related protein HRBL, which affects the CD4 down-regulation in a dual role as co-factor of both HIV-1 Nef and Vpu.
Keywords
HLA CLASS-I, TYPE-1 NEF, PLASMA-MEMBRANE, EH DOMAIN, T-CELLS, MODULATION, CLATHRIN, PROGRESSION, VPU, REPLICATION

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Citation

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MLA
Landi, Alessia et al. “The Human Immunodeficiency Virus (HIV) Rev-binding Protein (HRB) Is a Co-factor for HIV-1 Nef-mediated CD4 Downregulation.” JOURNAL OF GENERAL VIROLOGY 97.3 (2016): 778–785. Print.
APA
Landi, A., Garcia Timermans, C., Naessens, E., Vanderstraeten, H., Stove, V., & Verhasselt, B. (2016). The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation. JOURNAL OF GENERAL VIROLOGY, 97(3), 778–785.
Chicago author-date
Landi, Alessia, Cristina Garcia Timermans, Evelien Naessens, Hanne Vanderstraeten, Veronique Stove, and Bruno Verhasselt. 2016. “The Human Immunodeficiency Virus (HIV) Rev-binding Protein (HRB) Is a Co-factor for HIV-1 Nef-mediated CD4 Downregulation.” Journal of General Virology 97 (3): 778–785.
Chicago author-date (all authors)
Landi, Alessia, Cristina Garcia Timermans, Evelien Naessens, Hanne Vanderstraeten, Veronique Stove, and Bruno Verhasselt. 2016. “The Human Immunodeficiency Virus (HIV) Rev-binding Protein (HRB) Is a Co-factor for HIV-1 Nef-mediated CD4 Downregulation.” Journal of General Virology 97 (3): 778–785.
Vancouver
1.
Landi A, Garcia Timermans C, Naessens E, Vanderstraeten H, Stove V, Verhasselt B. The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation. JOURNAL OF GENERAL VIROLOGY. 2016;97(3):778–85.
IEEE
[1]
A. Landi, C. Garcia Timermans, E. Naessens, H. Vanderstraeten, V. Stove, and B. Verhasselt, “The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation,” JOURNAL OF GENERAL VIROLOGY, vol. 97, no. 3, pp. 778–785, 2016.
@article{7051995,
  abstract     = {HIV-1-mediated CD4 down-regulation is an important determinant of viral replication in vivo. Research on cellular co-factors involved in this process could lead to the identification of potential therapeutic targets. We found that CD4 surface levels were significantly higher in HIV-1-infected cells knocked-down for the HIV Rev-Binding protein (HRB) compared to control cells. HRB knock-down affected CD4 down-regulation induced by Nef but not by HIV-1 Vpu. Interestingly, the knock-down of the related protein HRBL (HRB Like), but not of the HRB interaction partner EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15), increased CD4 levels in Vpu-expressing cells significantly. Both these proteins are known to be involved in HIV-1-mediated CD4 down-regulation as cofactors of HIV-1 Nef. These results identify HRB as a previously unknown co-factor for HIV-1 Nef-mediated CD4 down-regulation and highlight differences with the related protein HRBL, which affects the CD4 down-regulation in a dual role as co-factor of both HIV-1 Nef and Vpu.},
  author       = {Landi, Alessia and Garcia Timermans, Cristina and Naessens, Evelien and Vanderstraeten, Hanne and Stove, Veronique and Verhasselt, Bruno},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keywords     = {HLA CLASS-I,TYPE-1 NEF,PLASMA-MEMBRANE,EH DOMAIN,T-CELLS,MODULATION,CLATHRIN,PROGRESSION,VPU,REPLICATION},
  language     = {eng},
  number       = {3},
  pages        = {778--785},
  title        = {The human immunodeficiency virus (HIV) Rev-binding protein (HRB) is a co-factor for HIV-1 Nef-mediated CD4 downregulation},
  url          = {http://dx.doi.org/10.1099/jgv.0.000382},
  volume       = {97},
  year         = {2016},
}

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