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Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox-sensitive genes

(2016) FASEB JOURNAL. 30(2). p.863-873
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Abstract
Neonates with intrauterine growth restriction (IUGR) show lower efficiency of nutrient utilization compared to normal birth weight (NBW) newborns. This study was conducted using neonatal piglets as a model to test the hypothesis that IUGR affects the intestinal barrier function, intestinal structure, and antioxidant system development during the suckling period. The small intestinal mucosae were obtained from IUGR and NBW littermates in the suckling period (d 0, 3, 8, and 19 postnatal). The epithelial barrier function was assessed by FITC-dextran 4 (FD4) and horseradish peroxidase (HRP) fluxes across the epithelium, histomorphologic measurements, and expression of tight-junction proteins. Redox status represented by the glutathione disulfide/glutathione ratio and malondialdehyde concentrations was determined, whereas mRNA expressions of some redox-sensitive proteins were quantified. Results showed that IUGR piglets exhibited a 2-fold higher intestinal permeability in the proximal small intestine on d 0 (P < 0.05), and this difference between IUGR and NBW piglets was widened to 3 and 4 times for FD4 and HRP, respectively (P < 0.05), on d 3. In accordance, expression of occludin was down-regulated at the transcriptional level in IUGR pigletsat d 0 and 19 (P < 0.01). Furthermore, the transcription of heme oxygenase 1, catalase, and thioredoxin reductase genes was down-regulated in IUGR piglets, mainly on postnatal d 0 and 19 (P < 0.01). It appears that IUGR subjects have a lower capacity to mount an antioxidant response in the early postnatal period. Collectively, these results add to our understanding of the mechanisms responsible for intestinal dysfunction in IUGR neonates.
Keywords
IUGR, intestinal tight junction, occludin, antioxidant capacity, BIRTH-WEIGHT PIGLETS, OXIDATIVE STRESS, HEME OXYGENASE, NEWBORN PIGS, GLUTATHIONE, TRANSPORT, PERFORMANCE, PLASMA, RATS, MALONDIALDEHYDE

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Citation

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Chicago
Wang, Wei, Jeroen Degroote, Christa Van Ginneken, Mario Van Poucke, Hans Vergauwen, Thi Minh Tho Dam, Daisy Vanrompay, Luc Peelman, Stefaan De Smet, and Joris Michiels. 2016. “Intrauterine Growth Restriction in Neonatal Piglets Affects Small Intestinal Mucosal Permeability and mRNA Expression of Redox-sensitive Genes.” Faseb Journal 30 (2): 863–873.
APA
Wang, W., Degroote, J., Van Ginneken, C., Van Poucke, M., Vergauwen, H., Dam, T. M. T., Vanrompay, D., et al. (2016). Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox-sensitive genes. FASEB JOURNAL, 30(2), 863–873.
Vancouver
1.
Wang W, Degroote J, Van Ginneken C, Van Poucke M, Vergauwen H, Dam TMT, et al. Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox-sensitive genes. FASEB JOURNAL. 2016;30(2):863–73.
MLA
Wang, Wei et al. “Intrauterine Growth Restriction in Neonatal Piglets Affects Small Intestinal Mucosal Permeability and mRNA Expression of Redox-sensitive Genes.” FASEB JOURNAL 30.2 (2016): 863–873. Print.
@article{7051759,
  abstract     = {Neonates with intrauterine growth restriction (IUGR) show lower efficiency of nutrient utilization compared to normal birth weight (NBW) newborns. This study was conducted using neonatal piglets as a model to test the hypothesis that IUGR affects the intestinal barrier function, intestinal structure, and antioxidant system development during the suckling period. The small intestinal mucosae were obtained from IUGR and NBW littermates in the suckling period (d 0, 3, 8, and 19 postnatal). The epithelial barrier function was assessed by FITC-dextran 4 (FD4) and horseradish peroxidase (HRP) fluxes across the epithelium, histomorphologic measurements, and expression of tight-junction proteins. Redox status represented by the glutathione disulfide/glutathione ratio and malondialdehyde concentrations was determined, whereas mRNA expressions of some redox-sensitive proteins were quantified. Results showed that IUGR piglets exhibited a 2-fold higher intestinal permeability in the proximal small intestine on d 0 (P < 0.05), and this difference between IUGR and NBW piglets was widened to 3 and 4 times for FD4 and HRP, respectively (P < 0.05), on d 3. In accordance, expression of occludin was down-regulated at the transcriptional level in IUGR pigletsat d 0 and 19 (P < 0.01). Furthermore, the transcription of heme oxygenase 1, catalase, and thioredoxin reductase genes was down-regulated in IUGR piglets, mainly on postnatal d 0 and 19 (P < 0.01). It appears that IUGR subjects have a lower capacity to mount an antioxidant response in the early postnatal period. Collectively, these results add to our understanding of the mechanisms responsible for intestinal dysfunction in IUGR neonates.},
  author       = {Wang, Wei and Degroote, Jeroen and Van Ginneken, Christa and Van Poucke, Mario and Vergauwen, Hans and Dam, Thi Minh Tho and Vanrompay, Daisy and Peelman, Luc and De Smet, Stefaan and Michiels, Joris},
  issn         = {0892-6638},
  journal      = {FASEB JOURNAL},
  keywords     = {IUGR,intestinal tight junction,occludin,antioxidant capacity,BIRTH-WEIGHT PIGLETS,OXIDATIVE STRESS,HEME OXYGENASE,NEWBORN PIGS,GLUTATHIONE,TRANSPORT,PERFORMANCE,PLASMA,RATS,MALONDIALDEHYDE},
  language     = {eng},
  number       = {2},
  pages        = {863--873},
  title        = {Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox-sensitive genes},
  url          = {http://dx.doi.org/10.1096/fj.15-274779},
  volume       = {30},
  year         = {2016},
}

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