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Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia

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Abstract
Seven strains, ICMP 19430(T), ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10-37 degrees C (optimum, 25-30 degrees C), at pH 4.0-9.0 (optimum, pH 6.0-7.0) and with 0-2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0-99.5 % sequence similarity to the closest type strain, Burkholderia phytofirmans PsJN(T), and 98.4-99.7 % sequence similarity to Burkholderia caledonica LMG 19076T. The predominant fatty acids were C-18 : 1 omega 7c (21.0 % of the total fatty acids in strain ICMP 19430(T)), C-16 : 0 (19.1 %), C-17 : 0 cyclo (18.9 %), summed feature 3 (C-16 : 1 omega 7c and/or C-16 :1 omega 6c; 10.7 %) and C-19 : 0 cyclo omega 8c (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430(T) was 63.2 mol%. The DNA-DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus Burkholderia was 55 % or less. On the basis of 16S rRNA and recA gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus Burkholderia, for which the name Burkholderia dipogonis sp. nov. is proposed, with the type strain ICMP 19430(T) (=LMG 28415(T)= HAMBI 3637(T)).
Keywords
MAXIMUM-LIKELIHOOD, DEOXYRIBONUCLEIC-ACID, DNA-SEQUENCES, MIMOSA SPP., BACTERIA, SOILS, NITROGEN, STRAINS, GENUS, TOOLS

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MLA
Sheu, Shih-Yi et al. “Burkholderia Dipogonis Sp. Nov., Isolated from Root Nodules of Dipogon Lignosus in New Zealand and Western Australia.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65.12 (2015): 4716–4723. Print.
APA
Sheu, S.-Y., Chen, M.-H., Liu, W. Y., Andrews, M., James, E. K., Ardley, J. K., De Meyer, S., et al. (2015). Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65(12), 4716–4723.
Chicago author-date
Sheu, Shih-Yi, Ming-Hui Chen, Wendy YY Liu, Mitchell Andrews, Euan K James, Julie K Ardley, Sofie De Meyer, et al. 2015. “Burkholderia Dipogonis Sp. Nov., Isolated from Root Nodules of Dipogon Lignosus in New Zealand and Western Australia.” International Journal of Systematic and Evolutionary Microbiology 65 (12): 4716–4723.
Chicago author-date (all authors)
Sheu, Shih-Yi, Ming-Hui Chen, Wendy YY Liu, Mitchell Andrews, Euan K James, Julie K Ardley, Sofie De Meyer, Trevor K James, John G Howieson, Bruna G Coutinho, and Wen-Ming Chen. 2015. “Burkholderia Dipogonis Sp. Nov., Isolated from Root Nodules of Dipogon Lignosus in New Zealand and Western Australia.” International Journal of Systematic and Evolutionary Microbiology 65 (12): 4716–4723.
Vancouver
1.
Sheu S-Y, Chen M-H, Liu WY, Andrews M, James EK, Ardley JK, et al. Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2015;65(12):4716–23.
IEEE
[1]
S.-Y. Sheu et al., “Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 65, no. 12, pp. 4716–4723, 2015.
@article{7051647,
  abstract     = {Seven strains, ICMP 19430(T), ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10-37 degrees C (optimum, 25-30 degrees C), at pH 4.0-9.0 (optimum, pH 6.0-7.0) and with 0-2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0-99.5 % sequence similarity to the closest type strain, Burkholderia phytofirmans PsJN(T), and 98.4-99.7 % sequence similarity to Burkholderia caledonica LMG 19076T. The predominant fatty acids were C-18 : 1 omega 7c (21.0 % of the total fatty acids in strain ICMP 19430(T)), C-16 : 0 (19.1 %), C-17 : 0 cyclo (18.9 %), summed feature 3 (C-16 : 1 omega 7c and/or C-16 :1 omega 6c; 10.7 %) and C-19 : 0 cyclo omega 8c (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430(T) was 63.2 mol%. The DNA-DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus Burkholderia was 55 % or less. On the basis of 16S rRNA and recA gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus Burkholderia, for which the name Burkholderia dipogonis sp. nov. is proposed, with the type strain ICMP 19430(T) (=LMG 28415(T)= HAMBI 3637(T)).},
  author       = {Sheu, Shih-Yi and Chen, Ming-Hui and Liu, Wendy YY and Andrews, Mitchell and James, Euan K and Ardley, Julie K and De Meyer, Sofie and James, Trevor K and Howieson, John G and Coutinho, Bruna G and Chen, Wen-Ming},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {MAXIMUM-LIKELIHOOD,DEOXYRIBONUCLEIC-ACID,DNA-SEQUENCES,MIMOSA SPP.,BACTERIA,SOILS,NITROGEN,STRAINS,GENUS,TOOLS},
  language     = {eng},
  number       = {12},
  pages        = {4716--4723},
  title        = {Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia},
  url          = {http://dx.doi.org/10.1099/ijsem.0.000639},
  volume       = {65},
  year         = {2015},
}

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