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Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules

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Abstract
In total, 31 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora root nodules and authenticated as rhizobia on this host. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, with the representative strains ICMP 19560(T), ICMP 19523(T), ICMP 19535(T), ICMP 19545(T) and ICMP 19512(T) being related most closely to Mesorhizobium sangaii SCAU7(T) (99.9-99.6 % similarity), Mesorhizobium cantuariense ICMP 19515(T) (99.7-99.6 %) and Mesorhizobium ciceri UMP-CA7(T) (99.7-99.5 %). Additionally, the novel strains formed distinct groups based on housekeeping gene sequence analysis and were closely related to Mesorhizobium waimense ICMP 19557(T) (93.5-94.9, 92.5-95.6 and 94.2-96.0 %), M. cantuariense ICMP 19515(T) (93.1-97.7, 93.5-95.4 and 94.8-96.8 %) and M. ciceri UMP-CA7(T) (93.2-97.2, 94.6-96.8 and 95.5-97.3 %) for glnII, recA and rpoB, respectively. Chemotaxonomic data supported the assignment of the strains to the genus Mesorhizobium, and DNA-DNA hybridizations, matrix-assisted laser desorption/ionization time-of-flight MS analysis, enterobacterial repetitive intergenic consensus PCR, physiological and biochemical tests allowed the genotypic and phenotypic differentiation from their nearest neighbouring species. Therefore, these strains represent five novel species for which the names Mesorhizobium calcicola sp. nov. (type strain ICMP 19560(T)=LMG 28224(T)=HAMBI 3609(T)), Mesorhizobium waitakense sp. nov. (type strain ICMP 19523(T)=LMG 28227(T)=HAMBI 3605(T)), Mesorhizobium sophorae sp. nov. (type strain ICMP 19535(T)=LMG 28223(T)=HAMBI 3606(T)), Mesorhizobium newzealandense sp. nov. (type strain ICMP 19545(T)=LMG 28226(T)=HAMBI 3607(T)) and Mesorhizobium kowhaii sp. nov. (type strain ICMP 19512(T)=LMG 28222(T)=HAMBI 3603(T)) are proposed.
Keywords
FLAVESCENS, TAXONOMY, BRADYRHIZOBIUM, STRAINS, FABACEAE, RHIZOBIUM-CICERI, NEW-ZEALAND, DEOXYRIBONUCLEIC-ACID, DIVERSE, DNA

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MLA
De Meyer, Sofie et al. “Mesorhizobium Calcicola Sp. Nov., Mesorhizobium Waitakense Sp. Nov., Mesorhizobium Sophorae Sp. Nov., Mesorhizobium Newzealandense Sp. Nov. and Mesorhizobium Kowhaii Sp. Nov. Isolated from Sophora Root Nodules.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66.2 (2016): 786–795. Print.
APA
De Meyer, Sofie, Tan, H. W., Andrews, M., Heenan, P. B., & Willems, A. (2016). Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 66(2), 786–795.
Chicago author-date
De Meyer, Sofie, Heng Wee Tan, Mitchell Andrews, Peter B Heenan, and Anne Willems. 2016. “Mesorhizobium Calcicola Sp. Nov., Mesorhizobium Waitakense Sp. Nov., Mesorhizobium Sophorae Sp. Nov., Mesorhizobium Newzealandense Sp. Nov. and Mesorhizobium Kowhaii Sp. Nov. Isolated from Sophora Root Nodules.” International Journal of Systematic and Evolutionary Microbiology 66 (2): 786–795.
Chicago author-date (all authors)
De Meyer, Sofie, Heng Wee Tan, Mitchell Andrews, Peter B Heenan, and Anne Willems. 2016. “Mesorhizobium Calcicola Sp. Nov., Mesorhizobium Waitakense Sp. Nov., Mesorhizobium Sophorae Sp. Nov., Mesorhizobium Newzealandense Sp. Nov. and Mesorhizobium Kowhaii Sp. Nov. Isolated from Sophora Root Nodules.” International Journal of Systematic and Evolutionary Microbiology 66 (2): 786–795.
Vancouver
1.
De Meyer S, Tan HW, Andrews M, Heenan PB, Willems A. Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2016;66(2):786–95.
IEEE
[1]
S. De Meyer, H. W. Tan, M. Andrews, P. B. Heenan, and A. Willems, “Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 66, no. 2, pp. 786–795, 2016.
@article{7051623,
  abstract     = {In total, 31 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora root nodules and authenticated as rhizobia on this host. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, with the representative strains ICMP 19560(T), ICMP 19523(T), ICMP 19535(T), ICMP 19545(T) and ICMP 19512(T) being related most closely to Mesorhizobium sangaii SCAU7(T) (99.9-99.6 % similarity), Mesorhizobium cantuariense ICMP 19515(T) (99.7-99.6 %) and Mesorhizobium ciceri UMP-CA7(T) (99.7-99.5 %). Additionally, the novel strains formed distinct groups based on housekeeping gene sequence analysis and were closely related to Mesorhizobium waimense ICMP 19557(T) (93.5-94.9, 92.5-95.6 and 94.2-96.0 %), M. cantuariense ICMP 19515(T) (93.1-97.7, 93.5-95.4 and 94.8-96.8 %) and M. ciceri UMP-CA7(T) (93.2-97.2, 94.6-96.8 and 95.5-97.3 %) for glnII, recA and rpoB, respectively. Chemotaxonomic data supported the assignment of the strains to the genus Mesorhizobium, and DNA-DNA hybridizations, matrix-assisted laser desorption/ionization time-of-flight MS analysis, enterobacterial repetitive intergenic consensus PCR, physiological and biochemical tests allowed the genotypic and phenotypic differentiation from their nearest neighbouring species. Therefore, these strains represent five novel species for which the names Mesorhizobium calcicola sp. nov. (type strain ICMP 19560(T)=LMG 28224(T)=HAMBI 3609(T)), Mesorhizobium waitakense sp. nov. (type strain ICMP 19523(T)=LMG 28227(T)=HAMBI 3605(T)), Mesorhizobium sophorae sp. nov. (type strain ICMP 19535(T)=LMG 28223(T)=HAMBI 3606(T)), Mesorhizobium newzealandense sp. nov. (type strain ICMP 19545(T)=LMG 28226(T)=HAMBI 3607(T)) and Mesorhizobium kowhaii sp. nov. (type strain ICMP 19512(T)=LMG 28222(T)=HAMBI 3603(T)) are proposed.},
  author       = {De Meyer, Sofie and Tan, Heng Wee and Andrews, Mitchell and Heenan, Peter B and Willems, Anne},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {FLAVESCENS,TAXONOMY,BRADYRHIZOBIUM,STRAINS,FABACEAE,RHIZOBIUM-CICERI,NEW-ZEALAND,DEOXYRIBONUCLEIC-ACID,DIVERSE,DNA},
  language     = {eng},
  number       = {2},
  pages        = {786--795},
  title        = {Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules},
  url          = {http://dx.doi.org/10.1099/ijsem.0.000796},
  volume       = {66},
  year         = {2016},
}

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