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Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry

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Abstract
Diacetoxyscirpenol (DAS), a Fusarium mycotoxin belonging to the trichothecene type A mycotoxins, is able to contaminate food and feed worldwide. Only limited information is available regarding the metabolism of DAS. The present study used ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry (UHPLC-Q/TOF) to investigate the in vitro phase I and II metabolism of DAS by rat, chicken, swine, goat, cow, and human liver microsomes. An extensive metabolization profile of DAS has been observed. A total of seven phase I and three phase II metabolites of DAS were detected. Among the identified molecules, four phase I metabolites (8 beta-hydroxy-DAS, neosolaniol, 7-hydroxy-DAS, and its epimer) and two phase II metabolites (4-deacetyl-DAS-3-glucuronic acid and 4-deacetyl-DAS-4-glucuronic acid) were identified for the first time. These results indicate that the major metabolic pathways of DAS in vitro were hydrolyzation (M1-M3), hydroxylation (M4-M7), and conjugation (M8-M10). Qualitative differences in phase I and II metabolic profiles of DAS between the five animal species and human were observed. 4-Deacetyl-DAS was the primary metabolite from liver microsomes of all species, especially human. The in vivo metabolism of DAS in rats and chickens after oral administration of DAS was also investigated and compared. The major metabolites for rats and chickens were 4-deacetyl-DAS and 7-hydroxy-DAS. These results will help to gain a more detailed insight into the metabolism and toxicity of DAS among different animal species and human.
Keywords
Diacetoxyscirpenol, Phase I and II metabolites, Metabolism, Animal, Human, UHPLC-Q/TOF, HUMAN LIVER-MICROSOMES, TRICHOTHECENE MYCOTOXINS, T-2 TOXIN, DRUG-METABOLISM, DEOXYNIVALENOL, RAT, CYTOTOXICITY, OCHRATOXIN, TOXICITY, PATHWAYS

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MLA
Yang, Shupeng et al. “Unraveling the in Vitro and in Vivo Metabolism of Diacetoxyscirpenol in Various Animal Species and Human Using Ultrahigh-performance Liquid Chromatography-quadrupole/time-of-flight Hybrid Mass Spectrometry.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 407.28 (2015): 8571–8583. Print.
APA
Yang, S., De Boevre, M., Zhang, H., De Ruyck, K., Sun, F., Wang, Z., Cao, X., et al. (2015). Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(28), 8571–8583.
Chicago author-date
Yang, Shupeng, Marthe De Boevre, Huiyan Zhang, Karl De Ruyck, Feifei Sun, Zhanhui Wang, Xingyuan Cao, Jianzhong Shen, Sarah De Saeger, and Suxia Zhang. 2015. “Unraveling the in Vitro and in Vivo Metabolism of Diacetoxyscirpenol in Various Animal Species and Human Using Ultrahigh-performance Liquid Chromatography-quadrupole/time-of-flight Hybrid Mass Spectrometry.” Analytical and Bioanalytical Chemistry 407 (28): 8571–8583.
Chicago author-date (all authors)
Yang, Shupeng, Marthe De Boevre, Huiyan Zhang, Karl De Ruyck, Feifei Sun, Zhanhui Wang, Xingyuan Cao, Jianzhong Shen, Sarah De Saeger, and Suxia Zhang. 2015. “Unraveling the in Vitro and in Vivo Metabolism of Diacetoxyscirpenol in Various Animal Species and Human Using Ultrahigh-performance Liquid Chromatography-quadrupole/time-of-flight Hybrid Mass Spectrometry.” Analytical and Bioanalytical Chemistry 407 (28): 8571–8583.
Vancouver
1.
Yang S, De Boevre M, Zhang H, De Ruyck K, Sun F, Wang Z, et al. Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2015;407(28):8571–83.
IEEE
[1]
S. Yang et al., “Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 407, no. 28, pp. 8571–8583, 2015.
@article{7048419,
  abstract     = {Diacetoxyscirpenol (DAS), a Fusarium mycotoxin belonging to the trichothecene type A mycotoxins, is able to contaminate food and feed worldwide. Only limited information is available regarding the metabolism of DAS. The present study used ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry (UHPLC-Q/TOF) to investigate the in vitro phase I and II metabolism of DAS by rat, chicken, swine, goat, cow, and human liver microsomes. An extensive metabolization profile of DAS has been observed. A total of seven phase I and three phase II metabolites of DAS were detected. Among the identified molecules, four phase I metabolites (8 beta-hydroxy-DAS, neosolaniol, 7-hydroxy-DAS, and its epimer) and two phase II metabolites (4-deacetyl-DAS-3-glucuronic acid and 4-deacetyl-DAS-4-glucuronic acid) were identified for the first time. These results indicate that the major metabolic pathways of DAS in vitro were hydrolyzation (M1-M3), hydroxylation (M4-M7), and conjugation (M8-M10). Qualitative differences in phase I and II metabolic profiles of DAS between the five animal species and human were observed. 4-Deacetyl-DAS was the primary metabolite from liver microsomes of all species, especially human. The in vivo metabolism of DAS in rats and chickens after oral administration of DAS was also investigated and compared. The major metabolites for rats and chickens were 4-deacetyl-DAS and 7-hydroxy-DAS. These results will help to gain a more detailed insight into the metabolism and toxicity of DAS among different animal species and human.},
  author       = {Yang, Shupeng and De Boevre, Marthe and Zhang, Huiyan and De Ruyck, Karl and Sun, Feifei and Wang, Zhanhui and Cao, Xingyuan and Shen, Jianzhong and De Saeger, Sarah and Zhang, Suxia},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {Diacetoxyscirpenol,Phase I and II metabolites,Metabolism,Animal,Human,UHPLC-Q/TOF,HUMAN LIVER-MICROSOMES,TRICHOTHECENE MYCOTOXINS,T-2 TOXIN,DRUG-METABOLISM,DEOXYNIVALENOL,RAT,CYTOTOXICITY,OCHRATOXIN,TOXICITY,PATHWAYS},
  language     = {eng},
  number       = {28},
  pages        = {8571--8583},
  title        = {Unraveling the in vitro and in vivo metabolism of diacetoxyscirpenol in various animal species and human using ultrahigh-performance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry},
  url          = {http://dx.doi.org/10.1007/s00216-015-9016-4},
  volume       = {407},
  year         = {2015},
}

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