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Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity

Marielle Van Zelst (UGent) , Myriam Hesta (UGent) , Kerry Gray and Geert Janssens (UGent)
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Abstract
Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.
Keywords
Selenium, Canine, Storage, GPx, Antioxidant, Freeze

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Citation

Please use this url to cite or link to this publication:

Chicago
Van Zelst, Marielle, Myriam Hesta, Kerry Gray, and Geert Janssens. 2016. “Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.” Biological Trace Element Research 172 (2): 361–363.
APA
Van Zelst, M., Hesta, M., Gray, K., & Janssens, G. (2016). Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity. BIOLOGICAL TRACE ELEMENT RESEARCH, 172(2), 361–363.
Vancouver
1.
Van Zelst M, Hesta M, Gray K, Janssens G. Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity. BIOLOGICAL TRACE ELEMENT RESEARCH. 2016;172(2):361–3.
MLA
Van Zelst, Marielle, Myriam Hesta, Kerry Gray, et al. “Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.” BIOLOGICAL TRACE ELEMENT RESEARCH 172.2 (2016): 361–363. Print.
@article{7041718,
  abstract     = {Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.},
  author       = {Van Zelst, Marielle and Hesta, Myriam and Gray, Kerry and Janssens, Geert},
  issn         = {0163-4984},
  journal      = {BIOLOGICAL TRACE ELEMENT RESEARCH},
  keyword      = {Selenium,Canine,Storage,GPx,Antioxidant,Freeze},
  language     = {eng},
  number       = {2},
  pages        = {361--363},
  title        = {Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity},
  url          = {http://dx.doi.org/10.1007/s12011-015-0603-1},
  volume       = {172},
  year         = {2016},
}

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