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Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity

Marielle Van Zelst, Myriam Hesta UGent, Kerry Gray and Geert Janssens UGent (2016) BIOLOGICAL TRACE ELEMENT RESEARCH. 172(2). p.361-363
abstract
Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Selenium, Canine, Storage, GPx, Antioxidant, Freeze
journal title
BIOLOGICAL TRACE ELEMENT RESEARCH
Biol. Trace Elem. Res.
volume
172
issue
2
pages
361 - 363
Web of Science type
Article
Web of Science id
000381203300012
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
2.399 (2016)
JCR rank
171/286 (2016)
JCR quartile
3 (2016)
ISSN
0163-4984
DOI
10.1007/s12011-015-0603-1
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7041718
handle
http://hdl.handle.net/1854/LU-7041718
date created
2016-01-18 15:09:55
date last changed
2018-03-02 14:04:09
@article{7041718,
  abstract     = {Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.},
  author       = {Van Zelst, Marielle and Hesta, Myriam and Gray, Kerry and Janssens, Geert},
  issn         = {0163-4984},
  journal      = {BIOLOGICAL TRACE ELEMENT RESEARCH},
  keyword      = {Selenium,Canine,Storage,GPx,Antioxidant,Freeze},
  language     = {eng},
  number       = {2},
  pages        = {361--363},
  title        = {Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity},
  url          = {http://dx.doi.org/10.1007/s12011-015-0603-1},
  volume       = {172},
  year         = {2016},
}

Chicago
Van Zelst, Marielle, Myriam Hesta, Kerry Gray, and Geert Janssens. 2016. “Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.” Biological Trace Element Research 172 (2): 361–363.
APA
Van Zelst, M., Hesta, M., Gray, K., & Janssens, G. (2016). Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity. BIOLOGICAL TRACE ELEMENT RESEARCH, 172(2), 361–363.
Vancouver
1.
Van Zelst M, Hesta M, Gray K, Janssens G. Storage of heparinised canine whole blood for the measurement of glutathione peroxidase activity. BIOLOGICAL TRACE ELEMENT RESEARCH. 2016;172(2):361–3.
MLA
Van Zelst, Marielle, Myriam Hesta, Kerry Gray, et al. “Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.” BIOLOGICAL TRACE ELEMENT RESEARCH 172.2 (2016): 361–363. Print.