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Anodes stimulate anaerobic toluene degradation via sulfur cycling in marine sediments

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Abstract
Hydrocarbons released during oil spills are persistent in marine sediments due to the absence of suitable electron acceptors below the oxic zone. Here, we investigated an alternative bioremediation strategy to remove toluene, a model monoaromatic hydrocarbon, using a bioanode. Bioelectrochemical reactors were inoculated with sediment collected from a hydrocarbon-contaminated marine site, and anodes were polarized at 0 mV and + 300 mV (versus an Ag/AgCl [3MKCl] reference electrode). The degradation of toluene was directly linked to current generation of up to 301 mAm(-2) and 431 mAm(-2) for the bioanodes polarized at 0 mV and + 300 mV, respectively. Peak currents decreased over time even after periodic spiking with toluene. The monitoring of sulfate concentrations during bioelectrochemical experiments suggested that sulfur metabolism was involved in toluene degradation at bioanodes. 16S rRNA gene-based Illumina sequencing of the bulk anolyte and anode samples revealed enrichment with electrocatalytically active microorganisms, toluene degraders, and sulfate-reducing microorganisms. Quantitative PCR targeting the alpha-subunit of the dissimilatory sulfite reductase (encoded by dsrA) and the alpha-subunit of the benzylsuccinate synthase (encoded by bssA) confirmed these findings. In particular, members of the family Desulfobulbaceae were enriched concomitantly with current production and toluene degradation. Based on these observations, we propose two mechanisms for bioelectrochemical toluene degradation: (i) direct electron transfer to the anode and/or (ii) sulfide-mediated electron transfer.
Keywords
16S RIBOSOMAL-RNA, MICROBIAL FUEL-CELLS, HYDROCARBON-CONTAMINATED SOIL, SULFATE-REDUCING CONDITIONS, SULFIDE REMOVAL, PETROLEUM-HYDROCARBONS, COMMUNITY STRUCTURE, ELECTRON-TRANSFER, BIOELECTROCHEMICAL SYSTEMS, ENHANCED BIODEGRADATION

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Chicago
Daghio, Matteo, Eleni Vaiopoulou, Sunil Anil Patil, Ana Suárez-Suárez, Ian M Head, Andrea Franzetti, and Korneel Rabaey. 2016. “Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments.” Applied and Environmental Microbiology 82 (1): 297–307.
APA
Daghio, M., Vaiopoulou, E., Patil, S. A., Suárez-Suárez, A., Head, I. M., Franzetti, A., & Rabaey, K. (2016). Anodes stimulate anaerobic toluene degradation via sulfur cycling in marine sediments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(1), 297–307.
Vancouver
1.
Daghio M, Vaiopoulou E, Patil SA, Suárez-Suárez A, Head IM, Franzetti A, et al. Anodes stimulate anaerobic toluene degradation via sulfur cycling in marine sediments. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2016;82(1):297–307.
MLA
Daghio, Matteo, Eleni Vaiopoulou, Sunil Anil Patil, et al. “Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.1 (2016): 297–307. Print.
@article{7039783,
  abstract     = {Hydrocarbons released during oil spills are persistent in marine sediments due to the absence of suitable electron acceptors below the oxic zone. Here, we investigated an alternative bioremediation strategy to remove toluene, a model monoaromatic hydrocarbon, using a bioanode. Bioelectrochemical reactors were inoculated with sediment collected from a hydrocarbon-contaminated marine site, and anodes were polarized at 0 mV and + 300 mV (versus an Ag/AgCl [3MKCl] reference electrode). The degradation of toluene was directly linked to current generation of up to 301 mAm(-2) and 431 mAm(-2) for the bioanodes polarized at 0 mV and + 300 mV, respectively. Peak currents decreased over time even after periodic spiking with toluene. The monitoring of sulfate concentrations during bioelectrochemical experiments suggested that sulfur metabolism was involved in toluene degradation at bioanodes. 16S rRNA gene-based Illumina sequencing of the bulk anolyte and anode samples revealed enrichment with electrocatalytically active microorganisms, toluene degraders, and sulfate-reducing microorganisms. Quantitative PCR targeting the alpha-subunit of the dissimilatory sulfite reductase (encoded by dsrA) and the alpha-subunit of the benzylsuccinate synthase (encoded by bssA) confirmed these findings. In particular, members of the family Desulfobulbaceae were enriched concomitantly with current production and toluene degradation. Based on these observations, we propose two mechanisms for bioelectrochemical toluene degradation: (i) direct electron transfer to the anode and/or (ii) sulfide-mediated electron transfer.},
  author       = {Daghio, Matteo and Vaiopoulou, Eleni and Patil, Sunil Anil and Su{\'a}rez-Su{\'a}rez, Ana and Head, Ian M and Franzetti, Andrea and Rabaey, Korneel},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {16S RIBOSOMAL-RNA,MICROBIAL FUEL-CELLS,HYDROCARBON-CONTAMINATED SOIL,SULFATE-REDUCING CONDITIONS,SULFIDE REMOVAL,PETROLEUM-HYDROCARBONS,COMMUNITY STRUCTURE,ELECTRON-TRANSFER,BIOELECTROCHEMICAL SYSTEMS,ENHANCED BIODEGRADATION},
  language     = {eng},
  number       = {1},
  pages        = {297--307},
  title        = {Anodes stimulate anaerobic toluene degradation via sulfur cycling in marine sediments},
  url          = {http://dx.doi.org/10.1128/AEM.02250-15},
  volume       = {82},
  year         = {2016},
}

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